LOCUS       sequence-356731-        8116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express GFP from a 1.1Synapsin 
            promoter ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..8116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3074..3098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3101..3110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3107..3109,3110..3112,3113..3826)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3107..3109,3110..3112,3113..3823)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3152..3173)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3413..3432)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3760..3781
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3827..3851)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3904..3945
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3969..4557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4022..4042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4440..4451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4637..4870
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4942..5063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4979..4998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5033..5052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5103..5238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5258..5277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5259..5277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5287..5304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5287..5303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5296..5318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5445..5900
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5532..5551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5742..5763
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5926..6030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6031..6099,6100..6891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6249..6268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7062..7650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7551..7570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7804..7821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7938..7959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7974..7979,7980..7997,7998..8004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8012..8028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8017..8039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8036..8052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8036..8052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8071..8091
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8073..8091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatggg ttttggctac gtccagagca gaggaatgag ggcatgtaga ctaaatatgt
     1981 tcgtgtggaa gaggctgaat acacatcaga gttagtgctg caggaaatgc ttctgcattg
     2041 catacccaga gtttccttgc tcatctgaga gcatgtgttt tttccagatg tgtgtacttg
     2101 tgtgagattc tctgggtgtg tgtcaatgtg ttgcctgaac gtgcattgct caatatgctc
     2161 atgtgtgtta ccctgggctt gtacatctac atatatacct ggatgcccgt gtgttctgtg
     2221 atgtacatat accctgtgtc attccttgtt tttctatttg tgttattcca tgtgttcctt
     2281 caggctctca ctacccaagt gtccacctcc gcctgtctgg tgatgtttac gctaccccgt
     2341 gctcttttct ttgcctgaca gtgttgtcgt ggaagacatc tcgccaggaa cactgcagta
     2401 aggagaattt ctagttttat gttcccctcc gagtatgctt ctatcccgac cctcaacccc
     2461 aaaatgcctt cagaggtgaa aatcaacact ggaaacacaa gtatctggga agggtaacaa
     2521 tgcaagttag cctgaggatt taggaggagg ctgaaaaaca gagtaggagc cttactacgg
     2581 gtccagaccc tacggacaag aacccccact cccactcccc aaattgcgca ttccctcccc
     2641 catcagaggg ggaggggaag aggatgcagc gcggcgcggc gcgtgcgcac tgtcggattt
     2701 agtaccgcgg acagagcctt cgcccccgct gccggcgcgc gccaccacct ccccagcacc
     2761 aaaggcgggc tgacgtcact ctccagccct ccccaaactc ccctacctca ccgccttggt
     2821 cgcgtccgtg cagcggtgag tccagtcggg ccgcaccaca agaggtgcaa gatagggggg
     2881 tgcaggcgcg accatacgct ctgcggcggc agagcctcag cgctgcctca gtctgcagcg
     2941 ggcagcagag gagtcgcgtc gtgccagaga gcgccgccgt gctcctgagc cccttgcgct
     3001 ccgcccccgc ggcccaccga cccactgccc cttggatcca ctagtccagt gtggtggaat
     3061 tctgcagata tcaacaagtt tgtacaaaaa agcaggctac gccaccatgg tgagcaaggg
     3121 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3181 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3241 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     3301 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3361 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3421 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3481 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3541 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3601 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3661 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3721 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3781 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaaccc agctttcttg
     3841 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     3901 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
     3961 tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4021 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4081 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4141 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4201 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4261 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4321 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4381 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4441 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4501 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4561 gcgatggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4621 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4681 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4741 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4801 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4861 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4921 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4981 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5041 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5101 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5161 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5221 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     5281 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5341 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5401 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5461 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5521 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5581 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5641 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5701 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5761 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5821 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5881 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5941 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6001 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6061 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6421 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6721 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6901 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6961 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7201 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7861 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7921 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7981 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8041 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     8101 ctggagctgc aagctt
//
