LOCUS       sequence-356733-       15707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SCPL35 protein tagged with mRFP, under control of native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15707)
  AUTHORS   Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW
  TITLE     Asymmetric distribution of extracellular matrix proteins in seed 
            coat epidermal cells of Arabidopsis is determined by polar 
            secretion.
  JOURNAL   Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. 
            eCollection 2021 Nov.
  PUBMED    34877448
REFERENCE   2  (bases 1 to 15707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-11-28"; volume: "5(11):e360. doi"; pages: " 
            10.1002/pld3.360. eCollection 2021 Nov"
COMMENT     Sequence Label: gSCPL35-mRFP
FEATURES             Location/Qualifiers
     source          1..15707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..362
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5895..5919)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5930..6607
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             5930..6607
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     6328..6347
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6522..6545
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      6629..6881
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6914..6930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6914..6930)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6927..6949)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6938..6954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6962..6968,6969..6986,6987..6992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7007..7028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(7064..7316)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7467..8492)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8544..8723)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    9229..9253
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9774..10565
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10811..11399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11300..11319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11553..11570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11585..11725
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11651..11670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11811..11833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12069..12263
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12329..13402)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13831..14460)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagcagg
      361 cttcgcagta gccgtgagat tataagaaga aaaactaaga ttcatagtga caaagaagga
      421 acaaagaaac agagtgtgta taattttttt gcagagagaa agagaaagag agataagaaa
      481 taatgaagtt gttagttaat ttagtaggtg aagaatgtga aatgagggaa gaaggattta
      541 agacgcataa gtttcgtgtt tggctctcaa tgtgaaattg ggtttgattt ggtgagctaa
      601 aaaaagttcg ttgctctttt ctaacggtct tttaaatttg tgtggctctt tattctcgtg
      661 ttttgctatg ttttcgtttt ggtcttttga aaggacgaat cttttggagg ggattaacta
      721 gaaaattagg aaaattagaa ttagggtttt agacttgagg attgttgata aaagagaaat
      781 tgacaggtgg gtagtgacgg atcacctaag ttcttgttta tttgttatac actttatttt
      841 attttgtcaa acaacttgtt taatttatac gtgtggaaat tggaattgga agaatattaa
      901 tagtgagtaa ttcaaataaa cagaattttt ttggatacta ctgtactatt ttaaacctgg
      961 cggtgaagcc gttgttgttt gaatttaggt tatctgcata tatctggtga ataattgttt
     1021 tcgaacaatt tgggcttaga aaacatatgg gcctatcaat aaagtatcgg gcctttgata
     1081 tatataaaat ttcattaacc tcattattaa tgtattttgg gccttacagg ccctctggcc
     1141 tcttctaggt gactatagca tcaataaatg attctcatta agccgccaat ttctctggcc
     1201 tcttctaggt gactatagca tcaataaatt attcccgaga caattgcgaa taaaccactt
     1261 tctttggaaa ttatcattga gatcaacata aaacataagc tttgtttata tgacaatgct
     1321 gttgttttaa agctctacgg tagtactaca catgtaattt aatgaatttt aattgatttc
     1381 taaacagtac gagcaatttg tgtgttgaat tcaactttga ttatatggtt tataaaacct
     1441 tgttttggga gatgaaattc tgaattaaga aaaataaaga agaaagcatt taatttgccg
     1501 atggctccat ctccatagcc atgtggttca gtctccgatg atgacatctc aagcgttgaa
     1561 attcaattcc atctttctgt aaaaggtgag aaaattggta gtgaaccaac catgttatcg
     1621 gagaccgtgg aggcacatta caaggaaatg attatgaaat gtgtttattg ttataacatt
     1681 ttcacgtgag tatcaaaaaa aaaatctcat gaaaaataaa agacttttaa agtttttttt
     1741 aatataaaaa aagacagtat aaagtagaag aagtgggggc gttttctcat ctgctcttca
     1801 tttcgataaa taattgaaaa gaacaaaaga aacaaaagag acgagaaaga gttttggcga
     1861 tgaagaagaa cgcactttgg ctactgtgca ttctggtgtt accggcgata gcctgtggta
     1921 ggaaaccgga gaagaaggta acaatttcat cctctgggag gaaggaagat gaccttgtaa
     1981 ccggtttgcc cggccaacca ccggttaact tcaaacacta cgccggttac gtgaatctgg
     2041 ggcctgaaca gaagcaaaag gcacttttct attggttctt cgaagctcaa caaaactctt
     2101 ctcgtcgtcc tctcgtcctt tggctcaatg gaggtcagtt tctcttcctc tctcgctcat
     2161 ttacataaag tatattttat ttgaagtagt aatacaaact tatcattgtt ctcaattgga
     2221 attttcattc gaaacttcat tgtttctgcg gttttacaaa tatgtttacc aattttaaaa
     2281 tgatagcacc ttttttgaaa cggatgatgt gatgattatg tttgcaataa aaaaatcagt
     2341 gtaatgaaag attaatgaac tctcaagctc gtgcctcgtg ggccatggta attaggttaa
     2401 tacatatgtc taatcacacc atcacgtatt gaccacaaaa ctctgtgcct gcaggccgtg
     2461 atgttgggaa aataatagat aaaacttgca cgaaaaagaa aaagaaaaaa cactaaacat
     2521 agagaaatgt tttcggcagt tgtattttct tggatacata attgaatttg gactaaaatt
     2581 ttaattattt atatagctaa ctcttagaaa acatcaagtt gtgtacattg tcctaccaaa
     2641 ctccatcgtc tgtctcctta ccctgccttg taacttgcca agcagttcct ttcgtttcag
     2701 taacaataaa attttactgt aattgtatcg caacgagata gtaaagggtc gtttcgatgc
     2761 agttgttttt gttttaaatt ttccaaaaat tacaagtcta tcaaatagcc aaaacttaaa
     2821 gtagtctatt gtttgtacag ttatatatcg ctattcttaa taatctgtaa cttatagatg
     2881 ccgtgtatgg cttgataaaa atagccagaa acaccattaa gtgatacact gatactattc
     2941 aacatttttg ttccttacta ttgcttgatg gagaagtgat aacaaatctg atacagttgg
     3001 gaatgccgac aaattttaat tgcctacttt aataatattt cgttttgtgg aacgcacttc
     3061 aacaatctgt agtctgtata cggtggaacg agtaggttct tgcctcttcg ggacaaatat
     3121 agtccagtcc tcgttttctt aacgtgaaat ctcaaaacca tagcttaaaa caagttgtat
     3181 ttgaaaatga gcttcgatct aattaagtga acaaaagtta ttcacaaaaa ttaaaattaa
     3241 cctgtttaaa tacatcatat tttttcgtga agattcagtg aaataaaaca ctatactata
     3301 acccaacaac atatttggaa aaaatatcaa cgaagtcgta tatggtccgg ctctctgaca
     3361 gatattggac ttgtatcatt caatcagtgt cctttttgtg taacattcaa tgtccaaatc
     3421 ttgtttctag agtgagtaaa gatcttttgt ctgtttcttc tttttctctc ggtagttgta
     3481 cattcaattt aacgaattta ctttatacgg tttgtctttt ttcccacacg gttcattcca
     3541 atattaggat atttagggat acgagaaata tcaacctagt cttttttatt ggggctagaa
     3601 atcggataag atgttgaata ttcaattata cacttagaaa taaacaaaaa gctatgtcta
     3661 ataataatgt ggagttgtgt acatgtctag tttgaacaga aagattgaaa tttgttaata
     3721 atgaggtatt acctttatag gatttgcgtg attgaagggt acacacgaat ccaatgtaat
     3781 caagagcaat agcaaaatct ttagttattt aatttctgta tacgaaaatg atatttgaat
     3841 cttgatagac aactcgggac aaaatactga cgtctagcta gctagtctca ttaattttat
     3901 tttttctcga caaatttagt tgtttagttt tggattttct tgacaaatct agcgatggaa
     3961 ttgaaaatag caattttctt tagtgaattg tattattaca tatttggtga aactcttaaa
     4021 ttattttcat cagtttaact aaaatccaaa atatgcagga ccgggttgct catcaatagc
     4081 ctatggagct gcacaagaac taggcccttt tcttgttcac gacaacggtg gcaaacttac
     4141 ctacaatcat ttctcttgga acaaaggtaa aatgaattat taccatacta tcagaaacat
     4201 ttgaattctt tagctaacat gtatactaat attaaccgtg gttacattaa aaaaaatgtt
     4261 tgtgcagagg ccaatatgtt gttcttggaa gcgccggttg gggttgggtt ctcgtatacc
     4321 aataactcaa tggatcttca aaagcttgga gatgaagtta ctgcatcgga ctctcttgct
     4381 ttcctgatca actggttcat gaaattcccc gagttccgat ccagcgagtt ctacatctct
     4441 ggcgagagtt acgccggtaa gtgctttatc cgattttaat tcttgattta tgaattatat
     4501 cacctaatat aaaagaatta atcttgtata ttaatattat ataaaacaaa gattgaatca
     4561 catgcttttg atcttctttt ctcaggtcac tatgttcctc agctcgcgga ggtaatttac
     4621 gacaggaaca agaaggttac gaaagactct agcattaatc tcaagggatt catggtatgc
     4681 actctatata tatgtatact actagtaagg ttacttgcga aacgaggaag caacgtttct
     4741 tgtgctataa gtaaacataa aaggtattcg tttgatgttg cagataggga acgcggtgat
     4801 caatgaggca acagacatgg caggactagt ggactacgca tggagccacg ctataatatc
     4861 ggacgaggtc cacaccagca tccatggctc gtgcagtttt gaggaagata ccaccaacaa
     4921 gaccgaacaa tgctacaaca atttcaaagg gttcatggat gcttacaatg atatcgatat
     4981 atacagtatc tacactcccg tttgcctctc ctctttgttg tcatcttctc caagaaaacc
     5041 taaaatcgtc gtgtctcctc gtctccttac ttttgacgta cgtaatctac aatacaatat
     5101 agtatcattg taacataatg taattaccat aatctctctt cttacttttt cttaccaatt
     5161 cttctctttt gatggtttag gatttgtggg acaagtttcc ggccgggtac gatccatgca
     5221 ctgaaagtta tgccgagaat tactttaacc ggaaagatgt gcaggttgca ctccatgcga
     5281 acgtcaccaa ccttccttat ccgtactctc cttgcaggtt agtcctcctt acgttacgtg
     5341 gtactaacta aaattaatag ctagctagag actttgtatg ttaagtttaa tgtcatgttt
     5401 ctaatgaaca aagtatatat atatatgatc ttgtgggtgt gattcagcgg agtaataaag
     5461 agatggagtg atgctccatc caccatgatt cccatcatac aaaagctttt gacgggtggc
     5521 ctccgtatct ggatctatag gtatctatat ctttctttaa ctgttttaaa aattacgtga
     5581 aaaattcata tatattgtca attagtttaa tgttcactgg ttcaatgttg gctatagtgg
     5641 agatacggat gggagagttc cggtaacatc tacacgttat agcataaaaa agatgggact
     5701 taaggtcgag tcaccgtgga ggtcatggtt ccacaagagt caagtagctg gatgggttga
     5761 gacttatgcc ggtggactaa attttgtcac ggtgagagga gccggtcacc aggttccggc
     5821 cttggctccg gcgcaatctc tcacattatt ctctcacttc atttcttctg tccccttgcc
     5881 ttcaaaacgg ttcgacccag ctttcttgta caaagtggtt gataacagca tggcctcctc
     5941 cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
     6001 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     6061 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca
     6121 gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     6181 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     6241 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     6301 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     6361 ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag
     6421 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa
     6481 gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca
     6541 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg
     6601 cgcctaagct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     6661 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     6721 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6781 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6841 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc
     6901 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6961 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7021 tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag taacatagat
     7081 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     7141 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     7201 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     7261 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
     7321 aaattcgggg gatcgatccc gcgaactgtg gacgagaact gtgccaccaa gcgtaaggcc
     7381 gttctctcgc atttgccttg ctaggctcgc gcgagttgct ggctgaggcg ttctcgaaat
     7441 cagctcttgt tcggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt
     7501 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     7561 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     7621 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     7681 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc
     7741 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     7801 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     7861 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     7921 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     7981 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     8041 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     8101 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     8161 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     8221 atgcaatagg tcaggctctc gctgaatgcc ccaatgtcaa gcacttccgg aatcgggagc
     8281 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     8341 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     8401 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     8461 acagacgtcg cggtgagttc aggctttttc atatcttatt gccccccggg atcagatctg
     8521 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
     8581 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
     8641 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag
     8701 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
     8761 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga tcccctgcgc
     8821 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     8881 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     8941 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     9001 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     9061 gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt
     9121 gaagctgatc cgtcgagatt aatagaaata aattcagcct aattcggcgt taattcagta
     9181 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     9241 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     9301 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     9361 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     9421 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     9481 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     9541 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     9601 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     9661 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     9721 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     9781 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     9841 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     9901 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     9961 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
    10021 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
    10081 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
    10141 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
    10201 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
    10261 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
    10321 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
    10381 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
    10441 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
    10501 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
    10561 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
    10621 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
    10681 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
    10741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10921 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    10981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11521 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11581 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    11641 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    11701 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    11761 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    11821 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
    11881 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
    11941 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
    12001 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
    12061 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
    12121 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
    12181 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
    12241 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
    12301 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
    12361 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
    12421 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
    12481 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
    12541 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
    12601 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
    12661 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
    12721 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
    12781 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
    12841 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
    12901 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
    12961 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
    13021 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
    13081 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
    13141 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
    13201 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
    13261 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    13321 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    13381 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
    13441 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    13501 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    13561 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
    13621 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
    13681 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
    13741 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
    13801 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
    13861 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
    13921 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
    13981 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
    14041 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
    14101 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
    14161 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
    14221 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    14281 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    14341 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    14401 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    14461 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    14521 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    14581 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    14641 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    14701 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    14761 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    14821 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    14881 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    14941 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    15001 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    15061 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    15121 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    15181 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    15241 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    15301 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    15361 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    15421 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    15481 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    15541 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    15601 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
    15661 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
