LOCUS       sequence-356735-       14711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RD21A protein tagged with mRFP, under control of native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14711)
  AUTHORS   Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW
  TITLE     Asymmetric distribution of extracellular matrix proteins in seed 
            coat epidermal cells of Arabidopsis is determined by polar 
            secretion.
  JOURNAL   Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. 
            eCollection 2021 Nov.
  PUBMED    34877448
REFERENCE   2  (bases 1 to 14711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-11-28"; volume: "5(11):e360. doi"; pages: " 
            10.1002/pld3.360. eCollection 2021 Nov"
COMMENT     Sequence Label: gRD21A-mRFP
FEATURES             Location/Qualifiers
     source          1..14711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..362
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4899..4923)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4934..5611
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4934..5611
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     5332..5351
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5526..5549
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      5633..5885
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5918..5934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5918..5934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5931..5953)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5942..5958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5966..5972,5973..5990,5991..5996))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6011..6032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(6068..6320)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6471..7496)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7548..7727)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    8233..8257
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8778..9569
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9815..10403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10304..10323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10557..10574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10589..10729
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10655..10674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10815..10837
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11073..11267
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11333..12406)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12835..13464)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagcagg
      361 cttcgactgt atatgtactg ttccatccaa aactttctgt ccatgtaatc gtatttcaac
      421 atgtcatgaa attttaccaa taactaatgc gcaaatttca ccgtcagaaa ttttatccca
      481 tctcgcaatt caactttaga ttggagaatt gaattcgctg gttcttcagc attggtatca
      541 tatgatgagt atattctact tgattgcaaa gtgtttacct ttttgcgtca atagattttg
      601 attgttttgt ttctcatggt gacttggagc atggaggtgg gtaggtgcgg ttcaaagcat
      661 taaaaaaaga aaatttacat gtagttttga tattcttttg tctcgcaccg tatcgtctcc
      721 attcaaagta tgtgtctatg attattgagt gtacatctta ttatgattca ttatagaatg
      781 tagttcccct taggctagga gcgttagttg tgtctcaatg tttttggcca tatgtacact
      841 agcgtgtaat aaagttctaa aatactaatt aatgattcgt tattcagtca tattatacgt
      901 ttcacattaa tactttttat tgaatgtata aattccaata tcgtcatcag tgtatctcta
      961 cacgcaaaac tattgttgag ttttttcttt tccaaaactc taaattacaa tattattttt
     1021 tttattttga taagtataat attcgatgtc acttgaatat aataattttt attaattctc
     1081 tgacgaaaaa aagagagcaa gtgttctatt gtcacaagct aactcttgaa agcattatat
     1141 taaagaagtt tgagtaaaat gaataaatta tagaaaatat tttgagtctt ttaatttcgg
     1201 aaggaatcat aataatagag acaacaatct aatttcataa tggtaacttg taagacttta
     1261 gattctatat tatcaaaaac tcataagaat ctcgagttac atgtaacaat cctatccacg
     1321 cgtcttttac aatacgtgag aacacgcgct cctatttgtg tggacatttt atttttggct
     1381 tgccaattag agatcagtga cgtctcatct cttcctaccc atattgtcta accaataatt
     1441 gaccatcaca tattcacatt tatagatttc atctttacac tattgttttt tttttcactt
     1501 atcaaaatat ctcaaccgac cctgaaatta attaggattt ttaaatcgga ttttggctca
     1561 agtttttgtt tttgaatatt taagaaaata aatacaatta agtataagtt tagagatgat
     1621 ttgttcattt atttgaagtt tttgattttt tttttcttat taaaatgaca aaaagaatga
     1681 tttgagttct tttctttttt ggctcaagat ttattttggc ccacttttct tttcaatatt
     1741 attaagaaaa cttgactcat aaatttttgt tgggaaaaag aggaaatgag aaaagggata
     1801 ataataacaa acaaaaaaat gatttgatgt tggtttgggt tccctctttt tagttcaaat
     1861 ataacatgag taatagtggt aaatgtacat tatttgaaaa attgtaaata atgcactatt
     1921 tactatttat tatctgattt gctactcttt tttttttcag tttcaattat agttcaacat
     1981 ggagttattt ttttgtcaaa ccccatatga tatcttatct acaaagaagc ccataaatca
     2041 cttatcttca tttattttag atccacttat ttttaattct ttttttctgt tttgattccg
     2101 tttcaacttg ttggatacat ataactttta ttgtttctct atcgtatcaa aaaaataatt
     2161 tggatttttc ctatcttttt tttcttctaa catacggatt ttttcctttc cgaaattgaa
     2221 ctttctaata aaaaaattcg gaacaagtca ccattatttt agaaaattaa aaagagtgac
     2281 gaacgaaaac attaaaatag aaaactaaca attattacac ttatctcatt tggcttttat
     2341 atatacccca ccaccatctc ttgtccatca tacaatcgtc ttcttcatca ttttctcttc
     2401 ttcttctcca agaaaacact gaagaagaaa tggggttcct taagccaacc atggcgattc
     2461 tttttctagc gatggtcgcc gtttcatcag ccgtggacat gtcaatcatc tcctacgacg
     2521 agaaacatgg cgtctccacc accggtggcc gtagcgaagc cgaggttatg agtatctacg
     2581 aggcatggtt ggtgaaacac ggcaaggctc agagccagaa ctctcttgtt gagaaagatc
     2641 gacggttcga gatctttaaa gacaatcttc gtttcgtcga tgaacataac gagaagaatc
     2701 ttagttatag attgggtttg actcgttttg cggatcttac taacgatgag tatagatcca
     2761 agtatcttgg agctaagatg gagaagaaag gtgagagaag gactagccta cggtacgagg
     2821 ctcgtgtcgg tgatgagcta ccggagtcta ttgactggag gaagaaagga gccgtggctg
     2881 aggtcaaaga tcagggtggt tgcggtaagt ttctactgga tctaaccatc atttttagtt
     2941 actcttatcg tagtatatca ttttttgatt caatcataaa gttttgaaac ttttggaaaa
     3001 tgacatattt gccccttccg gaaattatgg gtgttagtta gcaagacaaa actcaataaa
     3061 tgaaattaat agtactaata atttaacaaa tcatcatgtg tataggtcat tagtcattac
     3121 ttaattagag acgttgactt atagtctagt tatttttgct tgtgagctat agttgttttg
     3181 tcctaagcat aaaaataagg ttttacttgg ttacaggctc acagcatttc aaatgtgtac
     3241 attttggtta tgaatttcag ttataaggtt tatgaatgaa tctgattaac tataacatta
     3301 aatttcgtag tcatgattat gttagaaatt gcacattatt ttagtcctcc ttacagaaca
     3361 ttgatattag aatatatgtg ttcctgtttg atgttaatgt tttgttgtaa gctgatgttg
     3421 ttgacactta tgattggtaa tgaagggagt tgttgggcgt tttcaaccat tggagcagtg
     3481 gagggaataa accagatcgt aaccggagac ctaataacct tgtctgaaca agagttggtc
     3541 gattgtgaca cttcatacaa cgaaggttgt aacggaggtc ttatggacta tgcttttgag
     3601 ttcattatca agaatggtgg aatcgataca gacaaagatt atccttacaa gggtgttgat
     3661 ggaacttgtg accagatcag ggtaagtaca ttggtttttg ttttgacaag gaattacatt
     3721 tgattattaa cacaacttat gattcttata tcaatcctct tgtgggtttc ttgttacaga
     3781 aaaacgctaa agttgtcact atcgattcat atgaggatgt tccaacttac agcgaggaat
     3841 cgttgaagaa agcagttgct catcaaccca ttagcattgc cattgaggct ggtggtcgtg
     3901 cgttccagct ctatgattcg gtacaaattt aaccatcttc tcatagttct taggtgtttg
     3961 ttagttttta tgcatataaa tccatttcac ttctgttgtt aattgtatac gccaattaca
     4021 aacgaaagga gaaactctgt ttggtgtttg cgtttacact ttacaaaaga attctatgaa
     4081 aaattgaaaa aaatgttgct agtgtttgaa aatgtaaagt tttattcata tataaacgca
     4141 tgaaacaatt gcatctaata ggtggttgaa tattaagtag cttttcacta tttttggtct
     4201 caaaatgttc atattgttgt tagtcacctt tgtctctaat gtgttgatgt gatcttttca
     4261 tctgaagggt atatttgatg gaagttgtgg aacacaacta gaccacggag ttgtggcggt
     4321 tggatacgga actgagaacg gcaaagatta ctggattgtg agaaactcat ggggtaaaag
     4381 ctggggagag agtggatacc taaggatggc gcgtaacatt gcgtcttcat caggaaaatg
     4441 tggaatcgcg attgaacctt catacccgat aaagaatggc gaaaacccgc caaacccggg
     4501 accttcacct ccatctccca tcaagcctcc aacccaatgt gacagttact acacttgtcc
     4561 tgagagcaac acttgttgtt gtctgtttga gtatggcaag tattgctttg cttggggatg
     4621 ttgcccacta gaagcagcca cttgctgtga tgacaactat agttgctgcc ctcacgagta
     4681 cccggtttgt gaccttgatc aaggaacctg tttattggta agttctaatt cttccacttt
     4741 cagtaacaaa tatcaagatg gtaaataatg cttgaatctc acctgacctt gtttgggaat
     4801 gtatcatttg cagagcaaga acagtccatt tagtgttaag gccttaaagc gtaaacccgc
     4861 aacgccattc tggtcacaag gcagaaagaa cattgccgac ccagctttct tgtacaaagt
     4921 ggttgataac agcatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt
     4981 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5041 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     5101 cgcctgggac atcctgtccc ctcagttcca gtacggctcc aaggcctacg tgaagcaccc
     5161 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5221 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     5281 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     5341 gcagaagaag accatgggct gggaggcctc caccgagcgg atgtaccccg aggacggcgc
     5401 cctgaagggc gagatcaaga tgaggctgaa gctgaaggac ggcggccact acgacgccga
     5461 ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga
     5521 catcaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     5581 cgccgagggc cgccactcca ccggcgccta agcttagagc tcgaatttcc ccgatcgttc
     5641 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     5701 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     5761 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     5821 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     5881 agatcgggaa ttggttccgg aaccaattcg taatcatgtc atagctgttt cctgtgtgaa
     5941 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6001 ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc tatttctatt
     6061 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     6121 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     6181 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     6241 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     6301 tgccaaatgt ttgaacgatc ggggaaattc gggggatcga tcccgcgaac tgtggacgag
     6361 aactgtgcca ccaagcgtaa ggccgttctc tcgcatttgc cttgctaggc tcgcgcgagt
     6421 tgctggctga ggcgttctcg aaatcagctc ttgttcggtc ggcatctact ctattccttt
     6481 gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
     6541 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
     6601 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
     6661 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
     6721 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
     6781 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
     6841 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
     6901 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
     6961 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
     7021 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
     7081 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
     7141 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     7201 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa tgccccaatg
     7261 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
     7321 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     7381 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg
     7441 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct
     7501 tattgccccc cgggatcaga tctggattga gagtgaatat gagactctaa ttggataccg
     7561 aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag
     7621 tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt
     7681 aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa
     7741 cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc
     7801 gctcatgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     7861 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt
     7921 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc
     7981 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg
     8041 ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg
     8101 cgccctgagt gcttgcggca gcgtgaagct gatccgtcga gattaataga aataaattca
     8161 gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     8221 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     8281 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     8341 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     8401 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     8461 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
     8521 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     8581 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     8641 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     8701 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     8761 aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag
     8821 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     8881 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     8941 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     9001 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     9061 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     9121 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     9181 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     9241 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     9301 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     9361 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     9421 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     9481 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     9541 gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc
     9601 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc
     9661 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     9721 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9961 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10561 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
    10621 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10681 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    10741 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10801 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10861 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
    10921 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
    10981 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
    11041 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
    11101 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
    11161 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
    11221 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
    11281 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
    11341 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
    11401 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
    11461 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
    11521 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
    11581 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
    11641 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
    11701 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
    11761 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
    11821 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
    11881 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
    11941 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
    12001 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
    12061 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
    12121 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
    12181 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
    12241 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
    12301 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
    12361 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg
    12421 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
    12481 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
    12541 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
    12601 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
    12661 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
    12721 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
    12781 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
    12841 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
    12901 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
    12961 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
    13021 gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt
    13081 gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg
    13141 gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc
    13201 cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc
    13261 gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc
    13321 cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc
    13381 gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg
    13441 gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc
    13501 atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt
    13561 agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca
    13621 tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg
    13681 aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg
    13741 tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg
    13801 ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg
    13861 tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa
    13921 gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg
    13981 cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg
    14041 cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg
    14101 cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca
    14161 ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg
    14221 tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt
    14281 caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca
    14341 gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg
    14401 tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc
    14461 tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc
    14521 aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt
    14581 cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga
    14641 tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
    14701 cggataaacc t
//
