LOCUS       sequence-356737-       13797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ARA12 protein tagged with mRFP, under control of TBA2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW
  TITLE     Asymmetric distribution of extracellular matrix proteins in seed 
            coat epidermal cells of Arabidopsis is determined by polar 
            secretion.
  JOURNAL   Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. 
            eCollection 2021 Nov.
  PUBMED    34877448
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-11-28"; volume: "5(11):e360. doi"; pages: " 
            10.1002/pld3.360. eCollection 2021 Nov"
COMMENT     Sequence Label: cARA12-mRFP
FEATURES             Location/Qualifiers
     source          1..13797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1688..1712
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2610..2619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3985..4009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4020..4697
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4020..4697
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     4418..4437
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4612..4635
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      4719..4971
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5004..5020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5004..5020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5017..5039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5028..5044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5052..5058,5059..5076,5077..5082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5097..5118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5154..5406)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5557..6582)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6634..6813)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7319..7343
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7864..8655
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8901..9489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9390..9409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9643..9660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9675..9815
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9741..9760)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9901..9923
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10159..10353
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10419..11492)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11921..12550)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tggttttgtg ttccctacat
      361 cataccctat atatagtcac acacacctaa acacattttt acatcatttt taataatgtc
      421 cgtgtctgtg tgtattttct taattgttac agtgaaataa ataaatgtga aagagatttg
      481 gtttaaatta gataactatt ttacttgaga gtgtttaatc tgacgtacat attggttaat
      541 tcagttcaac gtcttatagt gacattagat attaatattg attagatatt ttcttacgta
      601 atgttctcaa atcggataaa tcagtcagtc aagccattat aataaaatat tatacgaagt
      661 tataaaagaa gaaaaagaac tttttactaa gtttagaatt tataatattc aattttcact
      721 ttactaacat cggtgattca cctaaagcat tctgttttac caagaatgtg tgtttgcttt
      781 agaattcaca gtatttactt ctcacttgat cccgtgtgta gtagacaata aaaccatgtt
      841 gtttcggaga ctcatcatat tcacaaacag attttccgat ttaatagttc tttttcttct
      901 tttataattt tcatagtact gtatcttgtt ttaatggctt tgccttgact gactgattta
      961 tctgatttga gaacactacg taagaaaaca tctaatacta ttatctacta tcactatatc
     1021 tgtatttaaa taacaatagt tgtttaccaa tatcaaaatt acagagggtg cttaactgcg
     1081 tttagtatac attttttatt atgtaatttt aattataaat aaaccagttt acaatttggg
     1141 atttctttat taggatttca taacacacca ttgttgaaaa atggagatct aaattgacat
     1201 tatatatgaa agacaataaa taagatacaa cgttttatat aaacaatatg gattatatac
     1261 aatagttaaa aaatccttag atgtctgtta aacacagcgt tttggtgagt taactcgtat
     1321 ggtataagtt taaagcattc cagtaactat attggtatca aaatctacat tcatattaat
     1381 tcaaataatt ttttacgttt accaaaataa atccatataa acatgcgatt gatcttgggc
     1441 aagagaggtg ttaaaatagt atcttggtat gactattagt tgaaatttga ctaatgcatt
     1501 caagaatgta tatttattgt aagaaatgct cacgtatctt atttgataaa gaatgctcac
     1561 gtaccttaat aaatacgtgt gcatagagtt tgtgtgtata tatagatggt tatgatcagg
     1621 gaagaagaac acaacacaac tcacctcaaa cagacaattt aattccaaaa gataaaaaca
     1681 atcaaaagca agtttgtaca aaaaagcagg ctatgtcttc ttcgtttctc tcctccactg
     1741 ctttcttcct cctcctttgt ctaggattct gccacgtgtc ctcttcctcc tccgaccaag
     1801 gaacttacat cgttcacatg gctaaatctc agatgccgtc gtctttcgac ctccactcta
     1861 actggtacga ttcatctctc agatctatct ccgactcagc cgaattgctc tacacttacg
     1921 agaacgcgat tcatggattc tcaacacggc taactcaaga agaagccgac tcgctcatga
     1981 ctcaacctgg tgttatctcc gttttaccgg agcaccgtta cgagctacac accactcgta
     2041 ctcctctctt cctcggtctc gacgaacaca ccgcagatct gttccctgaa gccggctctt
     2101 acagcgacgt cgtcgtcgga gttctcgata ccggagtttg gcccgagagt aaaagctact
     2161 ccgacgaagg attcggtcct attccttcct cctggaaagg cggatgcgag gccggaacca
     2221 atttcaccgc ttctctctgt aaccgtaaac taatcggagc aagattcttc gctcgtggtt
     2281 acgaatcaac gatgggacca atcgatgaat ctaaagaatc aagatctcct agagacgacg
     2341 acggtcacgg aactcacacc tcatcgaccg ccgctggatc cgtcgttgaa ggagctagct
     2401 tattaggcta cgcttcagga acagctcgtg gtatggctcc acgcgctcgt gtcgctgttt
     2461 acaaagtctg ttggctcggt ggttgtttca gctcagatat tttagctgca atcgataaag
     2521 ccatcgccga taacgtcaat gtcctgtcga tgtcactcgg cggtggaatg tcggattatt
     2581 atagagacgg tgttgcgatc ggagcattcg ccgccatgga aagagggatt ttagtatctt
     2641 gctcagctgg taatgctggt ccgagtagtt ctagtttatc aaacgtagct ccatggatca
     2701 caactgttgg tgctggtact ttagatcgtg attttccggc gcttgcgatt ctcggtaacg
     2761 gcaagaattt caccggagtt tctttgttta aaggagaagc tttaccggat aaattgttgc
     2821 cgtttattta cgctgggaat gctagtaatg ctactaatgg taatctctgt atgaccggaa
     2881 ctttgatccc ggagaaagta aaggggaaga ttgtgatgtg tgatagagga attaatgcta
     2941 gagttcagaa aggtgatgtg gttaaagcag caggtggagt tggaatgatt ctggctaaca
     3001 ctgcggcgaa tggtgaagag cttgttgcgg atgctcattt gttaccggcg accaccgttg
     3061 gtgaaaaagc cggtgatata atccggcatt acgtgactac tgatcctaat cccaccgctt
     3121 cgatttcaat cttaggaaca gtcgtcggtg ttaaaccatc tccggttgtc gcagcgttta
     3181 gctcacgtgg accgaattcg attacaccga atattcttaa accggatctg atcgctcctg
     3241 gagtaaacat cctcgccgcg tggaccggtg ctgctggacc aaccggactc gcttccgatt
     3301 ctcgccgcgt ggagttcaat atcatctctg ggacgtcgat gtcttgccct cacgttagtg
     3361 gtttagcggc gcttctcaag tcggtgcatc ctgaatggag cccggcggcg attagatcgg
     3421 cgcttatgac caccgcttac aaaacctaca aagacggtaa accgttactc gacatcgcga
     3481 cagggaagcc ttcgacgccg ttcgatcacg gtgcaggaca cgtgtcacca acaactgcca
     3541 ctaatccagg actcatctac gatctaacga cggaggatta cttaggcttc ctctgcgcat
     3601 tgaattacac atcgccgcaa attcgaagtg tctcgagacg taattacact tgcgatccga
     3661 gtaaatcgta ctcggtcgct gatttgaact acccgtcgtt cgccgttaac gttgatggag
     3721 tcggtgcgta taagtacacg cgcacggtga cgagcgtggg aggagctggg acttactcgg
     3781 ttaaagtaac ttcggagacg acaggagtca agatttcggt tgaaccggcg gttttgaatt
     3841 tcaaggaagc taacgagaaa aaatcgtata cggtgacgtt tactgtagac tcgtcgaagc
     3901 cgtctggatc taacagcttt gggagtattg aatggtcgga tgggaaacac gtggtgggaa
     3961 gtcccgtggc gattagctgg acatacccag ctttcttgta caaagtggtt gataacagca
     4021 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
     4081 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     4141 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     4201 tgtcccctca gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     4261 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     4321 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     4381 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     4441 tgggctggga ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga
     4501 tcaagatgag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     4561 acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca
     4621 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
     4681 actccaccgg cgcctaagct tagagctcga atttccccga tcgttcaaac atttggcaat
     4741 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     4801 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     4861 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     4921 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg
     4981 ttccggaacc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5041 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5101 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag
     5161 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     5221 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     5281 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     5341 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     5401 acgatcgggg aaattcgggg gatcgatccc gcgaactgtg gacgagaact gtgccaccaa
     5461 gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc gcgagttgct ggctgaggcg
     5521 ttctcgaaat cagctcttgt tcggtcggca tctactctat tcctttgccc tcggacgagt
     5581 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
     5641 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
     5701 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
     5761 gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc
     5821 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
     5881 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
     5941 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
     6001 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
     6061 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
     6121 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
     6181 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac
     6241 cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
     6301 acggcgggag atgcaatagg tcaggctctc gctgaatgcc ccaatgtcaa gcacttccgg
     6361 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
     6421 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
     6481 agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag
     6541 aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcttatt gccccccggg
     6601 atcagatctg gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg
     6661 aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac
     6721 ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc
     6781 cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg
     6841 tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga
     6901 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     6961 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     7021 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     7081 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     7141 ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt
     7201 gcggcagcgt gaagctgatc cgtcgagatt aatagaaata aattcagcct aattcggcgt
     7261 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     7321 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     7381 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     7441 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     7501 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     7561 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     7621 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     7681 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     7741 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     7801 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     7861 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     7921 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     7981 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     8041 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     8101 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     8161 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     8221 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     8281 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     8341 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     8401 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     8461 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     8521 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     8581 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     8641 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     8701 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     8761 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     8821 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     9061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9541 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9601 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9661 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9721 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     9781 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     9841 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     9901 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     9961 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
    10021 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
    10081 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
    10141 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
    10201 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    10261 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    10321 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
    10381 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    10441 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    10501 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    10561 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    10621 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    10681 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    10741 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    10801 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    10861 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    10921 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    10981 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    11041 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    11101 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    11161 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    11221 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
    11281 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    11341 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    11401 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    11461 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    11521 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    11581 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    11641 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    11701 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    11761 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    11821 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    11881 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    11941 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    12001 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    12061 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    12121 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    12181 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    12241 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    12301 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    12361 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    12421 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    12481 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    12541 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    12601 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    12661 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    12721 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    12781 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    12841 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    12901 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    12961 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    13021 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    13081 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    13141 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    13201 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    13261 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    13321 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    13381 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    13441 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    13501 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    13561 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    13621 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    13681 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    13741 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
