LOCUS       sequence-356738-       13026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ASPG1 protein tagged with mRFP, under control of TBA2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW
  TITLE     Asymmetric distribution of extracellular matrix proteins in seed 
            coat epidermal cells of Arabidopsis is determined by polar 
            secretion.
  JOURNAL   Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. 
            eCollection 2021 Nov.
  PUBMED    34877448
REFERENCE   2  (bases 1 to 13026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-11-28"; volume: "5(11):e360. doi"; pages: " 
            10.1002/pld3.360. eCollection 2021 Nov"
COMMENT     Sequence Label: cASPG1-mRFP
FEATURES             Location/Qualifiers
     source          1..13026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1688..1712
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3214..3238)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3249..3926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             3249..3926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     3647..3666
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3841..3864
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      3948..4200
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4233..4249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4233..4249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4246..4268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4257..4273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4281..4287,4288..4305,4306..4311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4326..4347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4383..4635)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4786..5811)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5863..6042)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6548..6572
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7093..7884
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8130..8718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8619..8638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8872..8889
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8904..9044
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8970..8989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9130..9152
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9388..9582
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9648..10721)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11150..11779)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tggttttgtg ttccctacat
      361 cataccctat atatagtcac acacacctaa acacattttt acatcatttt taataatgtc
      421 cgtgtctgtg tgtattttct taattgttac agtgaaataa ataaatgtga aagagatttg
      481 gtttaaatta gataactatt ttacttgaga gtgtttaatc tgacgtacat attggttaat
      541 tcagttcaac gtcttatagt gacattagat attaatattg attagatatt ttcttacgta
      601 atgttctcaa atcggataaa tcagtcagtc aagccattat aataaaatat tatacgaagt
      661 tataaaagaa gaaaaagaac tttttactaa gtttagaatt tataatattc aattttcact
      721 ttactaacat cggtgattca cctaaagcat tctgttttac caagaatgtg tgtttgcttt
      781 agaattcaca gtatttactt ctcacttgat cccgtgtgta gtagacaata aaaccatgtt
      841 gtttcggaga ctcatcatat tcacaaacag attttccgat ttaatagttc tttttcttct
      901 tttataattt tcatagtact gtatcttgtt ttaatggctt tgccttgact gactgattta
      961 tctgatttga gaacactacg taagaaaaca tctaatacta ttatctacta tcactatatc
     1021 tgtatttaaa taacaatagt tgtttaccaa tatcaaaatt acagagggtg cttaactgcg
     1081 tttagtatac attttttatt atgtaatttt aattataaat aaaccagttt acaatttggg
     1141 atttctttat taggatttca taacacacca ttgttgaaaa atggagatct aaattgacat
     1201 tatatatgaa agacaataaa taagatacaa cgttttatat aaacaatatg gattatatac
     1261 aatagttaaa aaatccttag atgtctgtta aacacagcgt tttggtgagt taactcgtat
     1321 ggtataagtt taaagcattc cagtaactat attggtatca aaatctacat tcatattaat
     1381 tcaaataatt ttttacgttt accaaaataa atccatataa acatgcgatt gatcttgggc
     1441 aagagaggtg ttaaaatagt atcttggtat gactattagt tgaaatttga ctaatgcatt
     1501 caagaatgta tatttattgt aagaaatgct cacgtatctt atttgataaa gaatgctcac
     1561 gtaccttaat aaatacgtgt gcatagagtt tgtgtgtata tatagatggt tatgatcagg
     1621 gaagaagaac acaacacaac tcacctcaaa cagacaattt aattccaaaa gataaaaaca
     1681 atcaaaagca agtttgtaca aaaaagcagg ctatggcttt cccgcgattt ctttctctcc
     1741 tcgccgtcgt cactctctct cttttcctca ccaccactga tgcttcctct cgctctctat
     1801 ccactccacc taaaacgaac gtactcgacg tcgtttcatc tctccagcaa acacaaacta
     1861 tcctctcact cgacccgact cgttcctcac tcaccaccac caaacccgaa tccctatccg
     1921 acccggtttt cttcaattca tcttcaccac tatctctaga gcttcactct cgagacactt
     1981 tcgtcgcgtc acagcacaaa gactacaaaa gcttaacact cagtcgactc gagcgcgact
     2041 catcccgagt cgccggaatc gtcgcgaaga tccgtttcgc cgtcgaagga gtcgatagat
     2101 ccgatctcaa acccgtttac aacgaagata cacgatacca aaccgaggat ctaacgacac
     2161 ctgtcgtctc cggagcctct caaggaagcg gcgagtattt ctcaagaatc ggtgtcggaa
     2221 ctccggcgaa agagatgtac ttggttctcg acaccggaag cgacgtaaat tggatccagt
     2281 gtgaaccttg cgccgactgt taccaacagt ccgacccggt ttttaacccg acttcgtctt
     2341 ccacatacaa atctctcact tgctctgctc cacagtgctc tcttctcgaa acctccgctt
     2401 gtcggtcaaa caaatgtctc taccaagtta gttacggaga cggatctttc accgtcggtg
     2461 aactcgctac cgatacggtg acgtttggta actctggtaa aataaacaac gtcgctttgg
     2521 gttgtggtca cgacaacgaa ggtctcttta ccggagcagc cggtttactc ggtttaggcg
     2581 gtggtgtttt atcgataacc aatcaaatga aagcgacgtc gttttcttac tgcctcgttg
     2641 accgtgactc gggaaagtcc tcaagtcttg atttcaactc ggtccaactc ggtggcggag
     2701 acgcgacggc gccgcttcta cgtaacaaaa agatcgacac tttctactac gtcggactca
     2761 gtggattcag cgtcggtggt gaaaaagtcg tgctgcctga tgcgatcttc gatgtcgatg
     2821 cttcgggaag cggaggagtg atcttggatt gtggaaccgc cgtgactcgt cttcagacgc
     2881 aggcttataa ctcgctgcgt gacgcgtttc ttaaactcac cgtgaatctc aaaaaaggat
     2941 cttcgagcat ctctctgttc gacacgtgtt acgatttctc gtctctctcc actgtgaaag
     3001 taccgacggt tgcgtttcat ttcacgggag ggaagtcgct tgatttgccg gcgaagaatt
     3061 atctgatccc tgtggatgat agtggaacgt tttgcttcgc ttttgctccg acgtcgtcgt
     3121 cgttgtcgat catcgggaat gtgcagcagc aagggacacg tatcacttac gatctatcca
     3181 aaaatgttat cggattgtca gggaataaat gctacccagc tttcttgtac aaagtggttg
     3241 ataacagcat ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
     3301 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3361 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3421 gggacatcct gtcccctcag ttccagtacg gctccaaggc ctacgtgaag caccccgccg
     3481 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3541 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     3601 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3661 agaagaccat gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga
     3721 agggcgagat caagatgagg ctgaagctga aggacggcgg ccactacgac gccgaggtca
     3781 agaccaccta catggccaag aagcccgtgc agctgcccgg cgcctacaag accgacatca
     3841 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgcgccg
     3901 agggccgcca ctccaccggc gcctaagctt agagctcgaa tttccccgat cgttcaaaca
     3961 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4021 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4081 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4141 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4201 gggaattggt tccggaacca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     4261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4321 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc
     4381 ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     4441 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     4501 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     4561 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     4621 aatgtttgaa cgatcgggga aattcggggg atcgatcccg cgaactgtgg acgagaactg
     4681 tgccaccaag cgtaaggccg ttctctcgca tttgccttgc taggctcgcg cgagttgctg
     4741 gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat ctactctatt cctttgccct
     4801 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     4861 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     4921 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     4981 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
     5041 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     5101 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     5161 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     5221 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     5281 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     5341 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     5401 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
     5461 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     5521 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaatgccc caatgtcaag
     5581 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     5641 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     5701 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     5761 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcttattg
     5821 ccccccggga tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg
     5881 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     5941 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     6001 ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     6061 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     6121 tgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     6181 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     6241 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     6301 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     6361 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     6421 tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta
     6481 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     6541 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     6601 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     6661 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     6721 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     6781 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     6841 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     6901 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     6961 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     7021 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     7081 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     7141 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     7201 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     7261 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     7321 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     7381 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     7441 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     7501 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     7561 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     7621 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     7681 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     7741 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     7801 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     7861 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     7921 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     7981 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     8041 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     8281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8821 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8881 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     8941 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9001 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     9061 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     9121 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     9181 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     9241 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     9301 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     9361 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     9421 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     9481 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     9541 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     9601 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     9661 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     9721 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     9781 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     9841 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     9901 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     9961 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
    10021 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
    10081 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
    10141 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
    10201 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
    10261 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
    10321 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
    10381 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
    10441 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
    10501 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
    10561 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
    10621 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
    10681 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
    10741 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
    10801 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
    10861 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
    10921 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
    10981 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
    11041 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
    11101 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
    11161 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
    11221 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
    11281 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
    11341 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
    11401 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
    11461 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
    11521 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
    11581 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
    11641 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    11701 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    11761 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    11821 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    11881 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    11941 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    12001 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    12061 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    12121 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    12181 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    12241 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    12301 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    12361 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    12421 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    12481 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    12541 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    12601 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    12661 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    12721 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    12781 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    12841 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    12901 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    12961 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    13021 aaacct
//
