LOCUS       sequence-356773-       11886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_W
FEATURES             Location/Qualifiers
     source          1..11886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        158..349
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     306..326
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(351..455)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      482..937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(569..588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     779..800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    944..1065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(955..974)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1009..1028
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(8358..9068,9069..9071,9072..9074))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKCEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8400..8421)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8749..8768
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9008..9029
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9071..9080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9107..9126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9108..9126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9171..9374)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9200..9220)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9375..9678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(9853..10441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9933..9952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    10770..10817
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     10842..10861
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(11049..11843)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(11049..11843)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11160..11179)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11770..11789
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(11878..11886,1..349))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
       61 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      121 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      181 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
      241 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
      301 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
      361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      481 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      541 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      601 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      661 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      721 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      781 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      841 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      901 aacgcgaatt ttaacaaaat attaacgctt acaatttacg cgttaagata cattgatgag
      961 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1021 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1081 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     1141 ctctacaaat gtggtatggc tgattatgat cagttatcta gatccggtgg atcccgggcc
     1201 cgcggctaac tggcacctgt cccttccatt acttgctggg cctgcttctg tcccatgcag
     1261 cactgtgcga ccgactggaa taacttttcg atttctgggg cacagtgtac aaattcatgg
     1321 ttccagaact taaacgctgg gtttttaatc agctcaatga aggtaataag aagaccccaa
     1381 ggatgtggcc tatttacaat caaccgttcc aagagaactc ttgtgatctg ttcttggatg
     1441 gcttccgtat tggcctctgc aaaaaggtac agcatggtgc aactgaagta gtgagtgtgg
     1501 ctatttgggt accggagctg atttgcaatt gcattcaaaa agagatagcg accctcagtg
     1561 tccaagtcca cagccaaatt ctggaagata tccatgtgtg ctgagtgagt gatggtgctc
     1621 attgaaggtg tgctgccctt gttgtggatg tgcgcaatgg cctgagtccc gacatagagc
     1681 accagtgcat tgatgagctg gaggttgtag cgattcccag gttcattgga tacctgtagg
     1741 ttgctgcgca gatcagacag gaaagtgact ggtgatcgag ttttaagata ggaatccaaa
     1801 tcctttttga actgaggtgg cattactcca gtgaaattgg tgagaatccg gggagcaatg
     1861 ttaatttcac tcaacatgtc cacctttaga ttaggagtga atgggtcggg gagcctcatg
     1921 tttcttggaa aggcactcag gatcaaattt cttaactgga tacaattagg tgggatcaca
     1981 tcacagaacc cataatggta atcacaaagg aactctggga aatcatgcaa aagaaccagc
     2041 agcactctta aagtgccctt gtagaggatt tgcataggtt tggtgagttc cacatttcta
     2101 aggaaaggcg ctaaatattt gaataaatca atcagtagct gtgcatacat aggccacccc
     2161 ttctgctgtg gcgtatgtgc cagcattctt gcaataaata tccgatggga aatcagttca
     2221 agccaggcat atacaaagcc aggagctttg gtaggcctca agatgtggaa tgtattgcag
     2281 aaagctgtaa gtgtctggaa attaatggtt tccaacacat gctcaggtgc attgagttcc
     2341 aagagaagca tgataaaaat tcgatggtag ggaagttgct gaaattcact ctgacgaaca
     2401 tcatgatcct gaaggagaac tcccactact ataccaagga ccttgttcag cagattaatc
     2461 tttgtgacag tgttggtggc ctcccctgag tgtttcacga gcagtgcaat gagtcgaaca
     2521 aaggcatcca ggttgtgata gcacttggct cggatcatgg tgggattggc agcaggattg
     2581 tgctgctgct cagcctgagc acggtaactg atttcaacac acatttcagt acacagacga
     2641 aagaaccttg ttatgagatc atcggtcttc agtattcctt gctggtgcat ctgtccaaca
     2701 aatgcagaga aagctttggt actgtcgcgg ccagctgctg ctgaatggta gagattcacc
     2761 cattccctca gaagatactc tgccttctcc ctcaggcctg gagggtcatc atactctgag
     2821 gcttgagaga tcccagaatg catcataaag tttgggcctc catgagcacg atcaatcatt
     2881 gcttcatagt tggatcgcac tacttccatc agctggggca atccttctgg agcattgcct
     2941 ctggaatgag cattaatcct catgagggtt tcaatggtgt ggaacagatc tgcctcagta
     3001 acatgagcaa cactcctttc atccaccagc aggattttta ctaactgcat agcaaatgcc
     3061 acagccatgt agtttaagcc attctccatt gactgcgcta ggtgaagatc atactgctgc
     3121 atattaacca aatgattgcg aattagcagc tccacagcct ccacattata tttatattca
     3181 tctcgacatt caattaggca ccttgtgatc tgtttgttgc accatggaga cccatatgcc
     3241 cggccatcct gcagagcttt taggaccaag aggtggcatt ccctgtagcg cagcagaagg
     3301 tcagcatcag caccacttgt ggcatctagt aagccctcta cagccttttg gagcaatcca
     3361 agagcagcta tggcatcccg agagtttcga gataaaacta caacctccaa gagacttcga
     3421 agagcctgag cttgagggtt catggccaaa gttggtggga tggcatgtag atgttgctcc
     3481 agttctgtaa tacacttatc ataaatctga gctacatcat ctgttgccca agcttgcttc
     3541 atgggctggg ctaaaaatcc cgtgggctga cttaagtcat ttgtaggtaa gaagccagga
     3601 acattgcgtg caaactcttc gtaaacagcc aactgctttg ggtccacacc accaactttc
     3661 agcctgattt gctctggcat ccgttcagca gcatatgtta aaacaacagg atcacagtat
     3721 ctgcgtcctt cttgcctagc atgttttctc agctcaaatt cagttgctaa tctcttgtcc
     3781 atctcagggc ctgctttttc tactgcagtc ttctgaataa aacagcaagc caactcacaa
     3841 ttgtcctgag ctaattgagc agctgcctga tccatcattt ctctttgttg tggggaagca
     3901 gtacgaaggg ctgaggcaaa actgtttttt aagttggtag atatgctcat gagcaaaggt
     3961 tcagcgcatg taatcatagc catgtaagct gtcaagttac gcatcatgtg atgagctgct
     4021 attcgcattc gagattcctc cgaatccagg gcaaaatccg acctgactat ttgctcacaa
     4081 gtagtcatgg caatcttaat tgatcgatcc accacaggat ggaccagctc ctggacagcc
     4141 cgttcaattg cctgacgcac acactgcttc aactgtggat gggcctgaaa caagggaatt
     4201 gttggattta gagtaatgtg tggtgccaag cccgcaaggg aatagacatt gatgtcgtgg
     4261 tagctgtact gtggctgtgg tggaaccgtg gctgtacaag tggtgttggt agctggtgta
     4321 gtagaagttg ttgtggttgt gatgggaggg agttcttctg gctgcttgac atctttcttt
     4381 ggagcagaga gttgctcatc taaattcttc aggcgatctt tatcctttag gaggtttcca
     4441 ggttttagct cattgatgtc taatgcaagg ttcttgcaga gaacctcgat ttcaaacttc
     4501 aagtttaact ttaagtcatg ctcctgatgt agctcagcta atacattcat aattgccatt
     4561 gtccaagggt ttggtggcct aaaaaccaca ctacgaatgc tagattctaa gactttggca
     4621 acaaagggca ctacatagag caattcttgt tgtcctttaa cataagcctc tagcagcaat
     4681 gatttcacat ccaagtcagt gtgtaagatg ggtttgtttt tagctaatgt gatcattcct
     4741 agccaatgtc ccaagttctt cagcaaagaa cgatctgaga aattggctgc agctttatca
     4801 gaggtcagga gcactttaat gtttctgtag gtctcattca gaaccatctt gttaaattca
     4861 ggattcttca gcgtgtcaag gaagtttgaa tacaggctat gaaagtttgg ctcaatactg
     4921 actctcttca taaccagata ctgtgaaacc caaggcataa attcttcttt caccgtttcc
     4981 tttagctctt caaccttttg tgtcatattt gactgtgaga gattattgaa aataaaagca
     5041 attttctcct ggatattttc tgggggctcc acaattctct cagtttgatc tgtggccaca
     5101 agcaacgtat ctatatttgt agtattaata gaaggtggca catctttctt aaagctgact
     5161 ccagttggcc tggtgaccgt aaccgtttta gcaacagtgg tagttgttga ggtggttacc
     5221 atagtgctaa cttgaccagc aagaggagct tttgctggaa cctgggcctg agcctgggcc
     5281 tgagccagtg caatacttcc aggggttgtg atagagcctt gcattttcac aggaggatct
     5341 ctagactgct gtccatactc aatatactcc tgtaaatgat gtggaaattg cataaagtga
     5401 ctgatagaag ccaaatgctg acaatactgg ggatagtcct tcaatctgtt tttaaatcta
     5461 tctagtgcag caatcccgaa ataatacatt ttggatccaa aaggcttgcg taaggcttca
     5521 agaacatatc gtagagccag acctagtgcc atgtaagtga ccagtccttt ctcaattata
     5581 ccaccaaata ggcaggctgt tatatgtaac tctttatcag gatactgggg aaaaaaacga
     5641 tattcttcaa acaagttcct tagcatacag ttaaatactt ctcgttccct ctttatagta
     5701 gagtctttaa atctctgcag catttctaat acctcatcaa cagacatggt tggatgtggt
     5761 ggatgattat atattcgctg gaaatagctg tttgcttcat catctatctc tttactaaag
     5821 tgctggtttg cctctggcca cacctgagac aagtccgaag gtttcatctt actctgctgg
     5881 aatgtaggct gattcagtcc agaggtgccc agtttcctct gtacaaaagg gtcgttattc
     5941 actgcaggga gtccaagagc accagttcct ataccagtca ggctgcctgt gccaagacca
     6001 cctactggaa gctgtgatga aagtcctcca ataccactga atgcagtggt ctgattgggg
     6061 gttgaaaggg gtggaaatgc ttttgctggt gactgagggg tactgaatgc agaacccaaa
     6121 tttggaggaa aaccctgcat actctgggtg tgaggggcag ctgaaccacc tatactaaga
     6181 gatgtcattc cagcaagagg gtcaatctga acaggagaaa tggcatctaa gctgctagca
     6241 ctaggaggac gtccttttgg cataactcca ggtggtggtt gtctggcctt attcataaca
     6301 ttactgcaat tggctaccat ggtgaggata gtttctgata gctcctgaga aacactccct
     6361 gcacaagctt gcagacaggc caacattgtc gccaaagttt ctggaggaag ttgagcactt
     6421 ttgggctggt ctttttctgg ggcaagtccg cccaaaatag aaggacaccg tctctttaaa
     6481 aaagtcatac acgcctggat aaaaggctcc ccatgctctc gaattttatc tgtgagccac
     6541 ttatcaagtt tgaggtattc acgacgtgaa gcaagtgcag caaggtcaat aacaaaggca
     6601 aatggagtac catttagcag cattgacaag gccttcaagt cctgggccac atcaagtatt
     6661 cgagacaatt tggcctgatc atactgctcc cctctcatgt accattctgc cattgcatgc
     6721 atgataagtt ggcgaattga gggagactgt ccctgcccat gccatgcata gtgcaaaata
     6781 atagctgagt taggatggtt tccaaggaaa attggcatca gagtggagat aagttcatgg
     6841 cgcaaggtat gccaagaggt gttaatttgt agtaaggcca ataccagcat gtctggacag
     6901 tgtttgatag ggaagctgaa gagctgtttg acttgctcat actgcccaac ctctgcaagc
     6961 ctcagcagag attcaatcaa atccaagctc ttccatgtgg caatttctcg attgtcatcc
     7021 tctggtggtg ctttcagaat atcagtggca acagtatgac agggatagtc agcaaaacag
     7081 aagatctctg gattgattag tgagtgctgg atgaaggaga gctggccttc agcatgtttc
     7141 caaggtctat atatgaggtc tactgggaac acttccatac ccaaacccct ctgaatgcca
     7201 taaaccacat tatgaagtcc tttactgtca cgaatttgaa atccaggatg gtccagttca
     7261 taagttactt ccttgaaatt caaacttgga ttcagttctt taagaacgtc aatcaagact
     7321 tctacattcc atgtgtgtgc ctgtgctcca tcacttttat ctttcccatc actccagatc
     7381 ccactgcccg gagcagaaat actctgtaat ggaatgccat ctgttaatcc tgaatgagtt
     7441 cgagccatca ttcccaaaac ccttgcaacc tgggcagctg tgacctcccg aacaccaaac
     7501 tgcacgatta tattgcgaca ttcttcaata cttgcacaaa agccatagcc tacttcttgc
     7561 atgaaatcag ccaaagagct ctccatcatg gttttagcta cccctccgga atcaggcagg
     7621 atcctgtcca ttagaatgtc ccgtttttca gggtataaaa gtggtgcgag caccacggga
     7681 cagcgttctt ggggaaaatc tctgcgcagc gtcttaagaa aagcgtctat ctgttcttgt
     7741 ccaactccaa aggctccctt ctgcccaaag aggagatggg agaggaggag gtgtaggacc
     7801 tctattgcta tatcttggaa gccaccttct tgatttccac tgacgtctgc gtcaatgtaa
     7861 gaacgcagaa gatctggaag cttctgtttg ataaactggg cagcgaaacc tctaagatct
     7921 gagctggaag aattcaacag ggcaaggcca aaaattacct cttgtacttt gcttaatttg
     7981 agcactttac tcagctgggc aaataagtgg ggtgcaggct ttaaactctt ctgatagtgc
     8041 aatggattat caatggcata ggacagcgtc gagataaaat ttggctttgt aatcagcaac
     8101 gcacactcct gaatcagaaa ctgagtctga tggaaatctt tgccactgct tttaccatcg
     8161 ccactgaaat ccacatgcga aaataggcag cgtaataaat gcctgtctgc ctcaggaccg
     8221 tgccgattca caatatgctg tatttcctgc tggctggctc ggtaattttt cttggttaaa
     8281 ttgtccacca ggtagctgat ttgagacaag gccagcgaga gcgagtcaag attcatagct
     8341 cgagatctga gtccggactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     8401 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     8461 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     8521 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     8581 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     8641 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     8701 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     8761 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     8821 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     8881 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     8941 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     9001 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     9061 cacttgctca ccatggtggc gaccggtagc gctagagctt gggtctccct atagtgagtc
     9121 gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt
     9181 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     9241 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     9301 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     9361 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     9421 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     9481 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     9541 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     9601 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     9661 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     9721 gattactatt aataactaat gcatggcggt aatacggtta tccacagaat caggggataa
     9781 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9841 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9901 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9961 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10021 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10081 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10141 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10201 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10261 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10321 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10381 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10441 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10501 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10561 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
    10621 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
    10681 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
    10741 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
    10801 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
    10861 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
    10921 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
    10981 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
    11041 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
    11101 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
    11161 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
    11221 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
    11281 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
    11341 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
    11401 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
    11461 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
    11521 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
    11581 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
    11641 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
    11701 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
    11761 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
    11821 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
    11881 gcaaaa
//
