LOCUS       sequence-356780-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Ng YH, Chanda S, Janas JA, Yang N, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Efficient generation of dopaminergic induced neuronal cells with 
            midbrain characteristics.
  JOURNAL   Stem Cell Reports. 2021 Jul 13;16(7):1763-1776. doi: 
            10.1016/j.stemcr.2021.05.017. Epub 2021 Jun 24.
  PUBMED    34171286
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.05.017"; journalName: "Stem Cell Reports"; 
            date: "2021-07-13- 13"; volume: "16"; issue: "7"; pages: "1763-1776"
COMMENT     Sequence Label: PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   213..275
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(716..734,735..757,758..776,777..799,800..818,819..841,
                     842..860,861..883,884..902,903..925,926..944,945..967,
                     968..986)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1019..1057
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1054..1078
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2347..2400
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2413..2422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3366..3383)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3372..3596
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3692..3903
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3916..4184
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4211..4810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4211..4230)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4707..4727
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4870..4890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4932..5053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4969..4988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5023..5042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5816..5850
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6341..6357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6341..6357)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6354..6376)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6365..6381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6389..6395,6396..6413,6414..6419))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6434..6455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6572..6589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6743..7331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6823..6842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7502..8293,8294..8362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8125..8144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8363..8467)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8493..8948)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8630..8651)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8842..8861
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt gaacccgtgg
      121 aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc
      181 tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat attgtgacgt
      241 acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
      301 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
      361 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca aaaactcaaa
      421 atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa agcccccagg
      481 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
      541 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
      601 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
      661 acctgggggg atacggggaa aaggcctcca cggccactag tctcgagttt accactccct
      721 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
      781 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      841 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      901 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      961 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg
     1021 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1081 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     1141 ccgcggcccc gaattcgaag actaggatcc gccaccatgc tggacggcct aaagatggag
     1201 gagaacttcc aaagcgcgat cgacacctcg gcctccttct cctcgctgct gggcagagcg
     1261 gtgagcccca agtctgtctg cgagggctgt cagcgggtca tcttggacag gtttctgctg
     1321 cggctcaacg acagcttctg gcatgagcag tgcgtgcagt gcgcctcctg caaagagccc
     1381 ctggagacca cctgcttcta ccgggacaag aagctgtact gcaagtatga ctacgagaag
     1441 ctgtttgctg ttaaatgtgg gggctgcttc gaggccatcg ctcccaatga gtttgttatg
     1501 cgggcccaga agagtgtata ccacctgagc tgcttctgct gctgtgtctg cgagcgacag
     1561 cttcagaagg gtgatgagtt tgtcctgaag gaggggcagc tgctctgcaa aggggactat
     1621 gagaaggagc gggagctgct cagcctggtg agcccagcag cctcagactc aggtaaaagt
     1681 gatgatgaag aaagtctctg caagtcagcc catggggcag ggaaaggaac tgctgaggaa
     1741 ggcaaggacc ataagcgccc caaacgtccg agaaccatct tgacaactca acagaggcga
     1801 gcattcaagg cctcatttga agtatcctcc aagccctgca ggaaggtgag agagactctg
     1861 gctgcagaga cagggctgag tgtccgtgtc gtccaggtgt ggttccaaaa ccagagagcg
     1921 aagatgaaga agctggccag gcgacagcag cagcagcagc aagatcagca gaacacccag
     1981 aggctgagct ctgctcagac aaacggtggt gggagtgctg ggatggaagg aatcatgaac
     2041 ccctacacgg ctctgcccac cccacagcag ctcctggcca tcgagcagag tgtctacagc
     2101 tcagatccct tccgacaggg tctcacccca ccccagatgc ctggagacca catgcaccct
     2161 tatggtgccg agcccctttt ccatgacctg gatagcgacg acacctccct cagtaacctg
     2221 ggtgactgtt tcctagcaac ctcagaagct gggcctctgc agtccagagt gggaaacccc
     2281 attgaccatc tgtactccat gcagaattct tacttcacat ctagatccat ggctagcggc
     2341 tccggcgagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca
     2401 gcatgctcta gagccaccat ggagtttggg ctgctcagcg aggcagaggc ccggagccct
     2461 gccctgtcgc tgtcagacgc tggcactccg cacccccagc tcccagagca cggctgcaag
     2521 ggccaggagc acagcgactc agaaaaggcc tcggcttcgc tgcccggcgg ctccccagag
     2581 gacggttcgc tgaaaaagaa gcagcggcgg cagcgcacgc acttcaccag ccagcagcta
     2641 caggagctag aggcgacctt ccagaggaac cgctaccccg acatgagcac gcgcgaggag
     2701 attgccgtgt ggaccaacct caccgaggcc cgcgtgcggg tgtggttcaa gaaccggcgc
     2761 gccaaatggc ggaagcgcga gcgcagccag caggccgagc tatgcaaagg cagcttcgcg
     2821 gcgccgctcg gggggctggt gccgccctac gaggaggtgt accccggcta ctcgtacggc
     2881 aactggccgc ccaaggctct tgccccgccg ctcgccgcca agacctttcc attcgccttc
     2941 aactcggtca acgtggggcc tctggcttcg cagcccgtct tctcgccacc cagctccatc
     3001 gccgcctcca tggtgccctc cgccgcggct gccccgggca ccgtgccagg gcctggggcc
     3061 ctgcagggcc tgggcggggg cccccccggg ctggctccgg ccgccgtgtc ctccggggcc
     3121 gtgtcctgcc cttatgcctc ggccgccgcc gccgccgcgg ctgccgcctc ttccccctac
     3181 gtctatcggg acccgtgtaa ctcgagcctg gccagcctgc ggctcaaagc caaacagcac
     3241 gcctccttca gctaccccgc tgtgcacggg ccgcccccgg cagccaacct tagtccgtgc
     3301 cagtacgccg tggaacggcc ggtgtgatta attaagctag ccctaggcta gagctcgctg
     3361 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3421 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3481 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3541 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3601 tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaacccttc gcggccgcga
     3661 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     3721 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     3781 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     3841 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     3901 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     3961 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4021 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4081 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4141 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     4201 cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4261 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4321 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4381 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4441 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4501 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4561 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4621 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4681 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4741 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4801 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4861 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4921 tgcgttaact aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5041 tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc
     5101 tcatctggtc tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt
     5161 cccaaactgg gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct
     5221 cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg
     5281 attccttttt tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag
     5341 gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc
     5401 cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga
     5461 gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg
     5521 ctttgggggg gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc
     5581 acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac
     5641 gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac
     5701 acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa
     5761 atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc
     5821 gtcaatttta cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc
     5881 gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa
     5941 gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac
     6001 tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg
     6061 gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg
     6121 cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat
     6181 accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg
     6241 ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata
     6301 ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7141 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8461 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     8521 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     8581 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     8641 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     8701 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     8761 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     8821 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     8881 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8941 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     9001 ctcttcgcta ttacgccagc tgg
//
