LOCUS       sequence-356781-       10006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Ng YH, Chanda S, Janas JA, Yang N, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Efficient generation of dopaminergic induced neuronal cells with 
            midbrain characteristics.
  JOURNAL   Stem Cell Reports. 2021 Jul 13;16(7):1763-1776. doi: 
            10.1016/j.stemcr.2021.05.017. Epub 2021 Jun 24.
  PUBMED    34171286
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.05.017"; journalName: "Stem Cell Reports"; 
            date: "2021-07-13- 13"; volume: "16"; issue: "7"; pages: "1763-1776"
COMMENT     Sequence Label: Piggybac-TetO-hNurr1-blast
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(159..164,165..182,183..189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     221..237
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        258..276
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   425..459
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        825..1139
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    833..851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    869..887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    904..922
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    940..958
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    976..994
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1011..1029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1047..1065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1112..1136
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3012..3032)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3430..3441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3682..3763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3719..3738)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        3783..4162
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4605..4627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4689..4708
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(4743..5360,5361..5366,5367..5402,5403..5405,5406..5441,
                     5442..5444,5445..5480,5481..5489)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(5508..6082,6083..6085,6086..6094)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5675..5692)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5902..5921
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6095..6493
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(6531..6548)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6537..6761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6808..6824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6832..6848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6856..6862,6863..6880,6881..6886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6901..6922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7324..7386
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7452..7471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7453..7471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7478..7495)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7478..7494)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7487..7509)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7636..8091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7723..7742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7933..7954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8117..8221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8222..8290,8291..9082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8440..8459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9253..9841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9742..9761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9995..10006,1..6)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      241 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt acctcgcgcg
      301 acttggtttg ccattcttta gcgcgcgtcg cgtcacacag cttggccaca atgtggtttt
      361 tgtcaaacga agattctatg acgtgtttaa agtttaggtc gagtaaagcg caaatctttt
      421 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
      481 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      541 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      601 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      661 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      721 tgttatagat atcgtgacta atatataata aaatgggtag ttctttagac gatgagcata
      781 tcctctctgc tcttctgcaa agcgatgacg agcttgttgg cttcgagttt actccctatc
      841 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
      901 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      961 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     1021 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     1081 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     1141 ggagaattgg ccaccatgcc ttgtgttcag gcgcagtatg ggtcctcgcc tcaaggagcc
     1201 agccccgctt ctcagagcta cagttaccac tcttcgggag aatacagctc cgatttctta
     1261 actccagagt ttgtcaagtt tagcatggac ctcaccaaca ctgaaatcac tgccaccact
     1321 tctctcccca gcttcagtac ctttatggac aactacagca caggctacga cgtcaagcca
     1381 ccttgcttgt accaaatgcc cctgtccgga cagcagtcct ccattaaggt agaagacatt
     1441 cagatgcaca actaccagca acacagccac ctgccccccc agtctgagga gatgatgccg
     1501 cactccgggt cggtttacta caagccctcc tcgcccccga cgcccaccac cccgggcttc
     1561 caggtgcagc acagccccat gtgggacgac ccgggatctc tccacaactt ccaccagaac
     1621 tacgtggcca ctacgcacat gatcgagcag aggaaaacgc cagtctcccg cctctccctc
     1681 ttctccttta agcaatcgcc ccctggcacc ccggtgtcta gttgccagat gcgcttcgac
     1741 gggcccctgc acgtccccat gaacccggag cccgccggca gccaccacgt ggtggacggg
     1801 cagaccttcg ctgtgcccaa ccccattcgc aagcccgcgt ccatgggctt cccgggcctg
     1861 cagatcggcc acgcgtctca gctgctcgac acgcaggtgc cctcaccgcc gtcgcggggc
     1921 tccccctcca acgaggggct gtgcgctgtg tgtggggaca acgcggcctg ccaacactac
     1981 ggcgtgcgca cctgtgaggg ctgcaaaggc ttctttaagc gcacagtgca aaaaaatgca
     2041 aaatacgtgt gtttagcaaa taaaaactgc ccagtggaca agcgtcgccg gaatcgctgt
     2101 cagtactgcc gatttcagaa gtgcctggct gttgggatgg tcaaagaagt ggttcgcaca
     2161 gacagtttaa aaggccggag aggtcgtttg ccctcgaaac cgaagagccc acaggagccc
     2221 tctccccctt cgcccccggt gagtctgatc agtgccctcg tcagggccca tgtcgactcc
     2281 aacccggcta tgaccagcct ggactattcc aggttccagg cgaaccctga ctatcaaatg
     2341 agtggagatg acacccagca tatccagcaa ttctatgatc tcctgactgg ctccatggag
     2401 atcatccggg gctgggcaga gaagatccct ggcttcgcag acctgcccaa agccgaccaa
     2461 gacctgcttt ttgaatcagc tttcttagaa ctgtttgtcc ttcgattagc atacaggtcc
     2521 aacccagtgg agggtaaact catcttttgc aatggggtgg tcttgcacag gttgcaatgc
     2581 gttcgtggct ttggggaatg gattgattcc attgttgaat tctcctccaa cttgcagaat
     2641 atgaacatcg acatttctgc cttctcctgc attgctgccc tggctatggt cacagagaga
     2701 cacgggctca aggaacccaa gagagtggaa gaactgcaaa acaagattgt aaattgtctc
     2761 aaagaccacg tgactttcaa caatgggggg ttgaaccgcc ccaattattt gtccaaactg
     2821 ttggggaagc tcccagaact tcgtaccctt tgcacacagg ggctacagcg cattttctac
     2881 ctgaaattgg aagacttggt gccaccgcca gcaataattg acaaactttt cctggacact
     2941 ttacctttct aagcggccgc tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3001 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3061 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3121 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3181 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3241 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3301 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3361 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3421 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3481 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccggg
     3541 ccgcatcgat aagcttgtcg acgatatctc tagaggatca taatcagcca taccacattt
     3601 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3661 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3721 aatagcatca caaatttcac aaataaagca tttttttcac tgcctcgata gcggcgcgcc
     3781 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3841 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3901 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3961 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4021 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4081 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4141 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     4201 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     4261 gatgggggcg gggggggggg gggggggctc gggggagggg cgcggcggcc cccggagcgc
     4321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     4381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     4441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     4501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     4561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     4621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     4681 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgatcca
     4741 ccatgtcgag actggacaag agcaaagtca taaacggcgc tctggaatta ctcaatggag
     4801 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     4861 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     4921 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     4981 ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     5041 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     5101 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     5161 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     5221 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     5281 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     5341 agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc
     5401 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     5461 attttgacct tgacatgctc cccgggtaag aattggccgc ggatccgccc ctctccctcc
     5521 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5581 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5641 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5701 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5761 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     5821 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     5881 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5941 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
     6001 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     6061 ctttgaaaaa cacgatgata atatggccac aaccatggcc aagcctttgt ctcaagaaga
     6121 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     6181 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     6241 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     6301 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     6361 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     6421 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     6481 gtgggagggc taagtcgagc ctctagagtt atctagagct cgctgatcag cctcgactgt
     6541 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6601 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6661 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6721 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6781 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6901 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6961 cctgtcgtgc cagcggatcc ttaattaacg agagcataat attgatatgt gccaaagttg
     7021 tttctgactg actaataagt ataatttgtt tctattatgt ataggttaag ctaattactt
     7081 attttataat acaacatgac tgtttttaaa gtacaaaata agtttatttt tgtaaaagag
     7141 agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     7201 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     7261 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     7321 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     7381 ctagggttaa ataatagttt ctaatttttt tattattcag cctgctgtcg tgaataccga
     7441 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     7501 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7561 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7621 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7681 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7741 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7801 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7861 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7921 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7981 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8041 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     8101 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8221 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8281 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8341 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8401 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8461 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8521 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8581 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8641 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8701 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8761 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8821 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8881 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8941 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9001 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9061 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9121 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9181 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9901 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9961 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtg
//
