LOCUS       sequence-356782-       11886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-IKGRQtoDSYAA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1-IKGRQtoDSYAA-shRNAres_W
FEATURES             Location/Qualifiers
     source          1..11886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKCEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(911..930)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1258..1279
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8614..8735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8651..8670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8705..8724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8742..9197)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8879..8900)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9091..9110
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9224..9328
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9330..9687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9330..9521
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9353..9373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9538..9673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9722..10516
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9722..10516
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9776..9795)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10386..10405
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10704..10723)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10748..10795
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11124..11712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11613..11632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggtg agcaagtgcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      901 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      961 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1021 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1081 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1141 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1201 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1261 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1321 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1381 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1441 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1501 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1561 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1621 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1681 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1741 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1801 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1861 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1921 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     1981 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2041 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2101 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2161 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2221 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2281 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2341 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2401 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2461 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2521 cctcatatat agaccttgga aacatgctga aggccagctc tccttcatcc agcactcact
     2581 aatcaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2641 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2701 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2761 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2821 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2881 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     2941 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3001 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3061 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3121 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3181 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3241 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3301 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3361 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3421 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3481 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3541 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3601 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3661 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3721 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3781 actgaatcag cctacattcc agcagagtaa gatgaaacct tcggacttgt ctcaggtgtg
     3841 gccagaggca aaccagcact ttagtaaaga gatagatgat gaagcaaaca gctatttcca
     3901 gcgaatatat aatcatccac cacatccaac catgtctgtt gatgaggtat tagaaatgct
     3961 gcagagattt aaagactcta ctataaagag ggaacgagaa gtatttaact gtatgctaag
     4021 gaacttgttt gaagaatatc gtttttttcc ccagtatcct gataaagagt tacatataac
     4081 agcctgccta tttggtggta taattgagaa aggactggtc acttacatgg cactaggtct
     4141 ggctctacga tatgttcttg aagccttacg caagcctttt ggatccaaaa tgtattattt
     4201 cgggattgct gcactagata gatttaaaaa cagattgaag gactatcccc agtattgtca
     4261 gcatttggct tctatcagtc actttatgca atttccacat catttacagg agtatattga
     4321 gtatggacag cagtctagag atcctcctgt gaaaatgcaa ggctctatca caacccctgg
     4381 aagtattgca ctggctcagg cccaggctca ggcccaggtt ccagcaaaag ctcctcttgc
     4441 tggtcaagtt agcactatgg taaccacctc aacaactacc actgttgcta aaacggttac
     4501 ggtcaccagg ccaactggag tcagctttaa gaaagatgtg ccaccttcta ttaatactac
     4561 aaatatagat acgttgcttg tggccacaga tcaaactgag agaattgtgg agcccccaga
     4621 aaatatccag gagaaaattg cttttatttt caataatctc tcacagtcaa atatgacaca
     4681 aaaggttgaa gagctaaagg aaacggtgaa agaagaattt atgccttggg tttcacagta
     4741 tctggttatg aagagagtca gtattgagcc aaactttcat agcctgtatt caaacttcct
     4801 tgacacgctg aagaatcctg aatttaacaa gatggttctg aatgagacct acagaaacat
     4861 taaagtgctc ctgacctctg ataaagctgc agccaatttc tcagatcgtt ctttgctgaa
     4921 gaacttggga cattggctag gaatgatcac attagctaaa aacaaaccca tcttacacac
     4981 tgacttggat gtgaaatcat tgctgctaga ggcttatgtt aaaggacaac aagaattgct
     5041 ctatgtagtg ccctttgttg ccaaagtctt agaatctagc attcgtagtg tggtttttag
     5101 gccaccaaac ccttggacaa tggcaattat gaatgtatta gctgagctac atcaggagca
     5161 tgacttaaag ttaaacttga agtttgaaat cgaggttctc tgcaagaacc ttgcattaga
     5221 catcaatgag ctaaaacctg gaaacctcct aaaggataaa gatcgcctga agaatttaga
     5281 tgagcaactc tctgctccaa agaaagatgt caagcagcca gaagaactcc ctcccatcac
     5341 aaccacaaca acttctacta caccagctac caacaccact tgtacagcca cggttccacc
     5401 acagccacag tacagctacc acgacatcaa tgtctattcc cttgcgggct tggcaccaca
     5461 cattactcta aatccaacaa ttcccttgtt tcaggcccat ccacagttga agcagtgtgt
     5521 gcgtcaggca attgaacggg ctgtccagga gctggtccat cctgtggtgg atcgatcaat
     5581 taagattgcc atgactactt gtgagcaaga tgtcaggtcg gattttgccc tggattcgga
     5641 ggaatctcga atgcgaatag cagctcatca catgatgcgt aacttgacag cttacatggc
     5701 tatgattaca tgcgctgaac ctttgctcat gagcatatct accaacttaa aaaacagttt
     5761 tgcctcagcc cttcgtactg cttccccaca acaaagagaa atgatggatc aggcagctgc
     5821 tcaattagct caggacaatt gtgagttggc ttgctgtttt attcagaaga ctgcagtaga
     5881 aaaagcaggc cctgagatgg acaagagatt agcaactgaa tttgagctga gaaaacatgc
     5941 taggcaagaa ggacgcagat actgtgatcc tgttgtttta acatatgctg ctgaacggat
     6001 gccagagcaa atcaggctga aagttggtgg tgtggaccca aagcagttgg ctgtttacga
     6061 agagtttgca cgcaatgttc ctggcttctt acctacaaat gacttaagtc agcccacggg
     6121 atttttagcc cagcccatga agcaagcttg ggcaacagat gatgtagctc agatttatga
     6181 taagtgtatt acagaactgg agcaacatct acatgccatc ccaccaactt tggccatgaa
     6241 ccctcaagct caggctcttc gaagtctctt ggaggttgta gttttatctc gaaactctcg
     6301 ggatgccata gctgctcttg gattgctcca aaaggctgta gagggcttac tagatgccac
     6361 aagtggtgct gatgctgacc ttctgctgcg ctacagggaa tgccacctct tggtcctaaa
     6421 agctctgcag gatggccggg catatgggtc tccatggtgc aacaaacaga tcacaaggtg
     6481 cctaattgaa tgtcgagatg aatataaata taatgtggag gctgtggagc tgctaattcg
     6541 caatcatttg gttaatatgc agcagtatga tcttcaccta gcgcagtcaa tggagaatgg
     6601 cttaaactac atggctgtgg catttgctat gcagttagta aaaatcctgc tggtggatga
     6661 aaggagtgtt gctcatgtta ctgaggcaga tctgttccac accattgaaa ccctcatgag
     6721 gattaatgct cattccagag gcaatgctcc agaaggattg ccccagctga tggaagtagt
     6781 gcgatccaac tatgaagcaa tgattgatcg tgctcatgga ggcccaaact ttatgatgca
     6841 ttctgggatc tctcaagcct cagagtatga tgaccctcca ggcctgaggg agaaggcaga
     6901 gtatcttctg agggaatggg tgaatctcta ccattcagca gcagctggcc gcgacagtac
     6961 caaagctttc tctgcatttg ttggacagat gcaccagcaa ggaatactga agaccgatga
     7021 tctcataaca aggttctttc gtctgtgtac tgaaatgtgt gttgaaatca gttaccgtgc
     7081 tcaggctgag cagcagcaca atcctgctgc caatcccacc atgatccgag ccaagtgcta
     7141 tcacaacctg gatgcctttg ttcgactcat tgcactgctc gtgaaacact caggggaggc
     7201 caccaacact gtcacaaaga ttaatctgct gaacaaggtc cttggtatag tagtgggagt
     7261 tctccttcag gatcatgatg ttcgtcagag tgaatttcag caacttccct accatcgaat
     7321 ttttatcatg cttctcttgg aactcaatgc acctgagcat gtgttggaaa ccattaattt
     7381 ccagacactt acagctttct gcaatacatt ccacatcttg aggcctacca aagctcctgg
     7441 ctttgtatat gcctggcttg aactgatttc ccatcggata tttattgcaa gaatgctggc
     7501 acatacgcca cagcagaagg ggtggcctat gtatgcacag ctactgattg atttattcaa
     7561 atatttagcg cctttcctta gaaatgtgga actcaccaaa cctatgcaaa tcctctacaa
     7621 gggcacttta agagtgctgc tggttctttt gcatgatttc ccagagttcc tttgtgatta
     7681 ccattatggg ttctgtgatg tgatcccacc taattgtatc cagttaagaa atttgatcct
     7741 gagtgccttt ccaagaaaca tgaggctccc cgacccattc actcctaatc taaaggtgga
     7801 catgttgagt gaaattaaca ttgctccccg gattctcacc aatttcactg gagtaatgcc
     7861 acctcagttc aaaaaggatt tggattccta tcttaaaact cgatcaccag tcactttcct
     7921 gtctgatctg cgcagcaacc tacaggtatc caatgaacct gggaatcgct acaacctcca
     7981 gctcatcaat gcactggtgc tctatgtcgg gactcaggcc attgcgcaca tccacaacaa
     8041 gggcagcaca ccttcaatga gcaccatcac tcactcagca cacatggata tcttccagaa
     8101 tttggctgtg gacttggaca ctgagggtcg ctatctcttt ttgaatgcaa ttgcaaatca
     8161 gctccggtac ccaaatagcc acactcacta cttcagttgc accatgctgt acctttttgc
     8221 agaggccaat acggaagcca tccaagaaca gatcacaaga gttctcttgg aacggttgat
     8281 tgtaaatagg ccacatcctt ggggtcttct tattaccttc attgagctga ttaaaaaccc
     8341 agcgtttaag ttctggaacc atgaatttgt acactgtgcc ccagaaatcg aaaagttatt
     8401 ccagtcggtc gcacagtgct gcatgggaca gaagcaggcc cagcaagtaa tggaagggac
     8461 aggtgccagt tagccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     8521 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8581 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8701 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     8761 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8821 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8881 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     8941 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     9001 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     9061 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     9121 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     9181 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9301 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     9361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9421 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9481 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9541 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9601 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9661 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     9721 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     9781 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     9841 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     9901 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     9961 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    10021 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    10081 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    10141 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    10201 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
    10261 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    10321 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
    10381 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    10441 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    10501 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
    10561 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    10621 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    10681 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    10741 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
    10801 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    10861 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    10921 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
    10981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11821 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    11881 gttccg
//
