LOCUS       sequence-356784-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mouse Wnt1 cDNA in TetO backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Ng YH, Chanda S, Janas JA, Yang N, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Efficient generation of dopaminergic induced neuronal cells with 
            midbrain characteristics.
  JOURNAL   Stem Cell Reports. 2021 Jul 13;16(7):1763-1776. doi: 
            10.1016/j.stemcr.2021.05.017. Epub 2021 Jun 24.
  PUBMED    34171286
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.05.017"; journalName: "Stem Cell Reports"; 
            date: "2021-07-13- 13"; volume: "16"; issue: "7"; pages: "1763-1776"
COMMENT     Sequence Label: TetO-FUW-mWnt1
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2824..2833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3973..4561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4026..4046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4444..4455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4564..4580)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4565..4581)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5086..5266
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5292..5309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5298..5522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5568..5996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5655..5674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5865..5886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6005..6025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6010..6339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6190..6325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6252..6271
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6387..6434
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6453..6827
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6957..7078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6994..7013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7048..7067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7127..7143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7127..7143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7140..7162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7151..7167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7175..7181,7182..7199,7200..7205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7220..7241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7358..7375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7609..7628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8288..9079,9080..9148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8911..8930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9149..9253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9328..9347)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcgccacca tggggctctg ggcgctgctg cccagctggg tttctactac gttgctactg
     2881 gcactgaccg ctctgcccgc agccctagct gccaacagta gtggccgatg gtggggcatc
     2941 gtgaacatag cctcctccac gaacctgttg acggattcca agagcctgca gctggtgctc
     3001 gagcccagtc tgcagctgct gagccgcaag cagcggcgac tgatccgaca gaacccgggg
     3061 atcctgcaca gcgtgagtgg agggctccag agcgccgtgc gagagtgcaa atggcaatyc
     3121 cgaaaccgcc gctggaactg ccccactgct ccggggcccc acctcttcgg caagatcgtc
     3181 aaccgaggct gccgagaaac agcgttcatc ttcgcaatca cctccgccgg ggtcacacat
     3241 tccgtggcgc gctcctgctc cgaaggctcc atcgagtcct gcacctgcga ctaccggcgg
     3301 cgcggccctg ggggccccga ctggcactgg gggggctgca gtgacaacat cgattttggt
     3361 cgcctctttg gccgagagtt cgtggactcc ggggagaagg ggcgggacct acgcttcctc
     3421 atgaaccttc acaacaacga ggcagggcga acgaccgtgt tctctgagat gcgccaagag
     3481 tgcaaatgcc acgggatgtc cggctcctgc acggtgcgca cgtgttggat gcggctgccc
     3541 acgctgcgcg ctgtgggcga cgtgctgcgc raccgcttcg acggtgcctc ccgcgtcctt
     3601 tacggcaaca gaggcagcaa ccgcgcctcg cgggcggagc tgctgcgcct ggagcccgaa
     3661 gaccccgcgc acaagcctcc ctcccctcac gacctcgtct acttcgagaa atcgcccaac
     3721 ttctgcacgt acagtggccg cctgggcaca gctggcacag ctggacgagc ttgcaacagc
     3781 tcgtctcccg cgctggacgg ctgtgagctg ctgtgctgtg gccgaggcca ccgcacgcgc
     3841 acgcagcgcg tcacggagcg ctgcaactgc accttccact ggtgctgcca cgtcagctgc
     3901 cgcaactgca cgcacacgcg cgttctgcac gagtgtctat gagctagcta attcgatatc
     3961 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4021 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4081 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4141 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4201 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4261 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4321 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4381 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4441 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4501 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4561 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     4621 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     4681 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     4741 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4801 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     4861 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4921 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     4981 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     5041 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     5101 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5161 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5221 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     5281 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5341 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5401 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5461 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5521 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5761 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5881 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6061 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6241 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6361 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     6421 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     6481 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     6541 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     6601 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     6661 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     6721 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     6781 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     6841 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6901 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6961 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7021 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7081 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7321 ggagaggcgg tttgcgtatt gggsgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9301 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9361 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     9421 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     9481 tgcgctgctt cgcgatgtac gggccagata ta
//
