LOCUS       sequence-356895-        8963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCDNA3.1 Construct GluN1 A714L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Cappelli J, Khacho P, Wang B, Sokolovski A, Bakkar W, Raymond S, 
            Ahlskog N, Pitney J, Wu J, Chudalayandi P, Wong AYC, Bergeron R
  TITLE     Glycine-induced NMDA receptor internalization provides 
            neuroprotection and preserves vasculature following ischemic stroke.
  JOURNAL   iScience. 2021 Dec 3;25(1):103539. doi: 10.1016/j.isci.2021.103539. 
            eCollection 2022 Jan 21.
  PUBMED    34977503
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2021-12-3"; volume: "25(1):103539. doi"; pages: " 
            10.1016/j.isci.2021.103539. eCollection 2022 Jan 21"
COMMENT     Sequence Label: pRB144
FEATURES             Location/Qualifiers
     source          1..8963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(105..124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        296..675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        676..879
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     830..850
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     924..943
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        924..942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1031..1741
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1058..1086)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1681..1701
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4615..4632)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4621..4845
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4891..5319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4978..4997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5188..5209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5328..5348)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5333..5662
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5513..5648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5575..5594
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5729..6523
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5783..5802)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6393..6412
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6697..6818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6734..6753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6788..6807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6867..6883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6867..6883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6880..6902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6891..6907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6915..6921,6922..6939,6940..6945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6960..6981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7098..7115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7269..7857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7349..7368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8028..8819,8820..8888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8651..8670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8889..8963,1..30))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
       61 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
      121 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      181 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      241 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      301 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      361 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      421 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      601 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      661 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      721 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      781 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      841 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      901 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      961 catgagcacc atgcacctgc tgacattcgc cctgcttttt tcctgctcct tcgcccgcgc
     1021 cgccactagg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1081 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1141 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc
     1201 aacacttgtc actactttaa cttatggtgt tcaatgcttt tcaagatacc cagatcatat
     1261 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1321 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1381 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1441 acacaaattg gaatacaact ataacgatca ccaggtgtac atcatggcag acaaacaaaa
     1501 gaatggaatc aaagctaact tcaaaattag acacaacatt gaagatggag gcgttcaact
     1561 agcagaccat tatcaacaaa atactccaat tggcgatggg cccgtccttt taccagacaa
     1621 ccattacctg tttacaactt ctactctttc gaaagatccc aacgaaaaga gagaccacat
     1681 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1741 agctagttgc gaccccaaga tcgtcaacat cggcgcggtg ctgagcacgc gcaagcatga
     1801 acagatgttc cgcgaggcag taaaccaggc caataagcga cacggctctt ggaagataca
     1861 gctcaacgcc acttctgtca cccacaagcc caacgccata cagatggccc tgtcagtgtg
     1921 tgaggacctc atctctagcc aggtctacgc tatcctagtt agccacccgc ctactcccaa
     1981 cgaccacttc actcccaccc ctgtctccta cacagctggc ttctacagaa tccctgtcct
     2041 gggactgact acccgaatgt ccatctactc tgacaagagt atccacctga gtttccttcg
     2101 cacggtgccg ccctactccc accagtccag cgtctggttt gagatgatgc gagtctacaa
     2161 ctggaaccac atcatcctgc tggtcagcga cgaccacgag ggacgggcag cgcagaagcg
     2221 cttggagacg ttgctggagg aacgggagtc caaggcagag aaggtgctgc agtttgaccc
     2281 aggaaccaag aatgtgacgg ctctgctgat ggaggcccgg gaactggagg cccgggtcat
     2341 catcctttct gcaagcgagg acgacgctgc cacagtgtac cgcgcagccg caatgctgaa
     2401 catgacgggc tctgggtacg tgtggctggt cggggaacgc gagatctctg ggaacgccct
     2461 gcgctacgct cctgatggca tcatcggact tcagctcatc aatggcaaga atgagtcagc
     2521 ccacatcagt gacgccgtgg gcgtggtggc acaggcagtt cacgaactcc tagagaagga
     2581 gaatatcact gacccaccgc ggggttgcgt gggcaacacc aacatctgga agacaggacc
     2641 attgttcaag agggtgctga tgtcttctaa gtatgcggac ggagtgactg gccgtgtgga
     2701 attcaatgag gatggggacc gcaaattcgc taattacagc atcatgaacc tgcagaaccg
     2761 caagctggtg caagtgggca tctacaatgg tacccatgtc atcccaaatg acaggaagat
     2821 catctggcca ggaggagaga cagagaaacc tcgaggatac cagatgtcca ccagactaaa
     2881 gatagtgaca atccaccaag agcccttcgt gtacgtcaag cccacaatga gtgatgggac
     2941 atgcaaagag gagttcacag tcaatggtga cccagtgaag aaggtgatct gtacggggcc
     3001 taatgacacg tccccaggca gcccacgcca cacagtgccc cagtgctgct atggcttctg
     3061 catagacctg ctcatcaagc tggcgcggac catgaatttt acctatgagg tgcacctggt
     3121 ggcagatggc aagtttggca cacaggagcg ggtaaacaac agcaacaaaa aggagtggaa
     3181 cggaatgatg ggcgagctac tcagtggcca agcggacatg attgtggcac cactgaccat
     3241 caacaatgag cgtgcgcagt acatagagtt ctccaagccc ttcaagtacc agggcctgac
     3301 cattttggtc aagaaggaga ttcccaggag cacactggac tcatttatgc agccttttca
     3361 gagcacactg tggttgctag taggactgtc agttcatgtg gtggctgtga tgctgtacct
     3421 gctggaccgc ttcagtccct ttggccgatt caaggtgaac agtgaggagg aggaggaaga
     3481 tgcactgacc ctgtcctctg ccatgtggtt ttcctggggc gtcctgctca actccggcat
     3541 tggggaaggt gccccccgga gtttctctgc acgtatccta ggcatggtgt gggctggttt
     3601 cgccatgatc atagtggctt cctacactgc caacttggca gctttcctgg tgctggatcg
     3661 gcctgaggag cgcatcacgg gcatcaatga ccccaggctc agaaacccct cagacaagtt
     3721 catctacgca actgtaaagc agagctccgt ggacatctac ttccggaggc aggtggagtt
     3781 gagtaccatg taccggcaca tggaaaaaca caattacgag agcctggctg aggccatcca
     3841 ggctgtgcgg gacaacaagc tgcacgcctt tatctgggac tcggccgtgc tggagtttga
     3901 ggcttcacag aagtgcgatc tggtgaccac gggtgagctg ttcttccgct caggctttgg
     3961 catcggcatg cgcaaggaca gcccctggaa gcagaacgtt tccctgtcca tactcaagtc
     4021 ccatgagaat ggcttcatgg aagatctgga taagacatgg gttcggtatc aggaatgcga
     4081 ctcccgcagc aatgctcctg caaccctcac ttttgagaac atggcagggg tcttcatgct
     4141 ggtggctgga ggcatcgtag ctgggatttt cctcattttc attgagatcg cctacaagcg
     4201 acacaaggat gcccgtagga agcagatgca gctggctttt gcagccgtga acgtgtggag
     4261 gaagaacctg caggatagaa agagtggtag agcagagccc gaccctaaaa agaaagccac
     4321 atttagggct atcacctcca ccctggcctc cagcttcaag agacgtaggt cctccaaaga
     4381 cacgagcacc gggggtggac gcggcgcttt gcaaaaccaa aaagacacag tgctgccgcg
     4441 acgcgctatt gagagggagg agggccagct gcagctgtgt tcccgtcata gggagagctg
     4501 agatatctaa tcacgaattc tggatccact agtccagtgt ggtggaattc tgcagatatc
     4561 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     4621 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4681 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4741 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4801 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4861 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4921 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4981 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5041 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5101 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5161 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5221 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5281 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5341 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5401 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5461 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5521 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5581 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5641 tggaggccta ggcttttgca aaaagctccc gggagcttat atatccattt tcggatctga
     5701 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5761 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5821 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5881 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5941 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6001 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6061 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6121 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6181 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6241 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6301 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6361 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6421 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6481 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6541 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6601 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6661 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6721 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6781 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6841 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7021 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7081 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7681 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8041 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8101 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8161 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8221 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8281 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8341 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8401 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8461 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8521 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8581 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8641 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8701 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8761 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8821 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8881 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8941 agcggataca tatttgaatg tat
//
