LOCUS       sequence-356897-       18015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be 
            cleaved by DUBs to express untagged VPS13D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18015)
  AUTHORS   Baldwin HA, Wang C, Kanfer G, Shah HV, Velayos-Baeza A, 
            Dulovic-Mahlow M, Bruggemann N, Anding A, Baehrecke EH, Maric D, 
            Prinz WA, Youle RJ
  TITLE     VPS13D promotes peroxisome biogenesis.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212018. doi: 
            10.1083/jcb.202001188.
  PUBMED    33891012
REFERENCE   2  (bases 1 to 18015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212018. doi"; 
            pages: " 10.1083/jcb.202001188"
COMMENT     Sequence Label: pGFP-Ub-VPS13D
FEATURES             Location/Qualifiers
     source          1..18015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1369..1593
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    14803..14924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14840..14859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14894..14913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(14931..15386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15068..15089)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15280..15299
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        15413..15517
                     /gene="bla"
                     /label=AmpR promoter
     promoter        15519..15876
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        15519..15710
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(15542..15562)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      15727..15862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             15911..16705
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             15911..16705
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15965..15984)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     16575..16594
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(16893..16912)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    16937..16984
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      17313..17901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17802..17821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcgg gccgcctgca gatcttcgtg
     1381 aagactctga ctggtaagac catcaccctc gaggttgagc ccagtgacac cattgagaat
     1441 gtcaaggcaa agatccaaga taaggaaggc atccctcctg accagcagag gctgatcttt
     1501 gctggaaaac agctggaaga tgggcgcacc ctgtctgact acaacatcca gaaagagtcc
     1561 accctgcacc tggtactccg tctcagaggt gggatgttgg aaggccttgt agcctgggtt
     1621 ctcaatacct atttgggaaa atatgtcaat aacctgaaca ctgaccagct ctcagttgca
     1681 cttctcaaag gtgctgttga attagaaaac ttgccattaa agaaagatgc cttgaaagaa
     1741 ttggaattac catttgaagt caaagctggc ttcattggga aagtaaccct tcagattccc
     1801 ttttatcgcc cccatgtgga cccttgggtg atctccatct ccagccttca cttaattgga
     1861 gccccagaga aaatacagga tttcaatgat gaaaaggaga agctgttgga aagggaacgt
     1921 aagaaagcac tacttcaagc cctggaggag aaatggaaga atgaccgcca gcagaaaggg
     1981 gagtcctatt ggtattcagt taccgcctcc gtagttacaa ggattgtgga gaatattgaa
     2041 ttaaaaattc aagatgtcca tttacgcttt gaagatggtg tcaccaatcc ctcccatcct
     2101 tttgcttttg gcatctgcat taagaatgtg tccatgcaaa atgctgtgaa tgagcctgta
     2161 cagaaactaa tgcggaaaaa gcaattagac gtagcagaat ttagcatcta ttgggatgtc
     2221 gattgcactt tactggggga tttgcctcag atggagttac aggaggccat ggccaggagc
     2281 atggagagtc gcagccatca ctacgtcctg gagcctgtgt ttgcatctgc tcttttgaag
     2341 agaaactgct ccaagaagcc cctgcggtct cggcacagtc cccgtattga ttgtgatatt
     2401 cagctggaga ccattccctt gaaactctct cagctgcaat accggcaaat catggaattc
     2461 ctcaaggagc tggaacgaaa ggagaggcag gtgaagttcc gaaggtggaa acccaaggtg
     2521 gcgatatcta agaactgccg agaatggtgg tattttgctt tgaatgctaa cttgtatgag
     2581 atcagagagc agaggaaacg ttgcacctgg gactttatgt tgcaccgcgc tcgtgatgct
     2641 gtatcttaca ctgacaaata tttcaacaag ttaaaaggag gcctgctgtc cacagatgac
     2701 aaggaggaaa tgtgtcggat tgaagaggaa cagagctttg aggaattgaa gattttgcgt
     2761 gaactggttc atgatcgatt tcacaaacag gaagaactag cagagagtct gcgggagcct
     2821 cagtttgatt ctccaggagc ctgtccggga gccccagaac ccggtggagg cagtgggatg
     2881 ctgcagtatc tccagtcctg gtttcctgga tggggtggct ggtacgggca gcagacccca
     2941 gaagggaatg tggttgaggg actgtcagca gagcaacagg agcagtggat tcctgaagag
     3001 atcctgggca ctgaggagtt ttttgacccc actgcagatg cctcgtgtat gaacacgtat
     3061 acaaagcgag atcatgtctt tgccaaactg aatttgcagt tgcagcgagg tacagtgact
     3121 ctgttacaca aggagcaagg aactcctcaa atgaatgaaa gtgctttcat gcagctcgag
     3181 ttttcagatg taaaacttct agcagagtct cttcctcgaa gaaattcctc gttgctttca
     3241 gtccggttgg gtggactgtt tcttcgagac ctggctacag aaggaactat gtttcctctt
     3301 ctagtcttcc ctaatccaca aaaagaagtt ggcagagtct cacaatcttt tggtctacaa
     3361 actacatctg cagacagaag tgatcattac ccagctgcag atccagatgg ccccgttttt
     3421 gagatgctgt atgagagaaa tccggcgcac agccactttg agaggcggct caatgtcagc
     3481 acaaggccct tgaacatcat atacaatccg caggccatta aaaaagtagc agactttttc
     3541 tacaagggaa aggttcatac ctcaggtttt ggttatcagt ctgaacttga gctgagagtg
     3601 gctgaagctg cccgaagaca atataacaag ctgaagatgc agaccaaggc agaaatccgg
     3661 caaactcttg atcgtttgct agtgggtgat ttcattgagg agagtaaacg atggaccgtg
     3721 cggctggata tttctgcccc tcaggtgata tttcctgatg atttcaaatt caagaatcct
     3781 gtgttagttg tcgtggatct aggaagaatg cttttgacga acacccaaga taactccagg
     3841 aggaaaagta gggatgggtc agcatctgaa gagacccagt ttagtgatga tgaatataag
     3901 acccccctgg ccacacctcc taacacccca cctcccgagt caagcagcag caacggagag
     3961 aaaacacctc ccttttctgg agttgagttc agtgaagaac agcttcaagc acatttaatg
     4021 agcacaaaga tgtatgagag gtactcgctg tcatttatgg acctccagat catggttgga
     4081 cgagtgaaag acaattggaa gcatgtccag gatattgacg tgggaccaac acatgtggta
     4141 gagaagttca acgttcacct acagttagag cgtcgattga tttatacttc agatcccaaa
     4201 tatccaggag ccgtgctctc aggcaactta ccagacttaa aaatccacat taatgaagat
     4261 aaaatatctg cactaaagaa ttgctttgct ctcctcacca ccccagaaat gaaaacttct
     4321 gacactcaga ttaaagaaaa gatttttccc caggaggagc agcggggaag tttgcaagac
     4381 tccgtaatga atttaaccca gagcattgtg ttgttggagc agcatacccg cgaggttctg
     4441 gtggagtcgc agctcctcct ggcggaattt aaagtgaact gtatgcagct tggtgttgag
     4501 agcaatggcc ggtacatttc tgtgctcaag gtgtttggta ccaatgctca ctttgtgaag
     4561 aggccttatg atgctgaagt ctccctaact gttcatggtt tgctcctggt ggataccatg
     4621 cagacatatg gtgctgattt tgaccttttg atggcttcac ataagaactt gagctttgat
     4681 attccaacgg gaagccttcg ggatagcagg gcccagtctc ctgtctctgg accgaatgtg
     4741 gcccacttaa ctgatggagc tacactgaac gaccgatcag ctactagtgt ttcacttgac
     4801 aaaattctta ccaaagagca agagtccctt attaagttgg aatatcagtt tgtgagttca
     4861 gagtgcccat cgatgaattt agacagtact cttcaggtga tttccctaca ggtgaataat
     4921 ttagatatta tcctcaatcc agagacgatt gtggagctaa ttggttttct tcaaaaatcc
     4981 tttcccaagg aaaaagatga tttaagtcct caacctttaa tgactgattt tgaaagaagc
     5041 ttcagagaac aaggaactta ccagtctaca tatgaacaaa acactgaggt tgcagtggaa
     5101 atccataggc tgaacttact gcttcttcgg acagtgggca tggcaaatag agagaaatat
     5161 ggcagaaaaa ttgcaactgc aagtataggt ggcaccaaag ttaatgtctc aatgggtagc
     5221 acgtttgaca tgaatggttc tcttggctgt ttacagctta tggatttgac acaagataac
     5281 gttaaaaacc agtatgttgt cagcattggg aattctgtag gctatgaaaa tatcatcagt
     5341 gatattggct actttgaatc tgtgtttgtc agaatggaag atgcagccct cactgaagct
     5401 ttgagtttca cgtttgttga gagatctaaa caggagtgtt ttctcaacct gaagatggct
     5461 tctttacatt ataaccactc tgctaagttt ttgaaggagt tgacgttatc catggatgaa
     5521 ctggaagaaa attttcgagg tatgctgaaa agcgcagcca ccaaagtcac cacagtacta
     5581 gctaccaaga ctgccgagta tagcgagatg gtatcgctct ttgaaactcc aaggaagact
     5641 cgggaaccct ttatcttaga ggaaaatgaa atatatgggt ttgacctagc ttcgtctcat
     5701 ttggacactg taaagctaat cttgaacata aacattgaat caccagttgt ttctatccct
     5761 cggaagccgg ggagtcctga gttgttggtg ggacacttgg gacagatatt catccagaat
     5821 tttgtggcgg gagatgatga atccagaagt gaccgtctgc aggtggaaat caaggacatt
     5881 aaactgtatt ctttgaattg cacccagttg gcaggtagag aagctgttgg gtctgaagga
     5941 agccggatgt tttgcccacc ttccgggtct ggcagtgcca acagtcagga ggaagctcat
     6001 ttcacacgac atgatttctt tgaatctttg catagaggtc aagcttttca catcctgaac
     6061 aacaccacca ttcagtttaa actggagaag atccctatag agagagaatc tgaattgact
     6121 ttttctctta gcccagatga cctgggaact tctagcatca tgaagattga aggaaaattt
     6181 gtcaatccag ttcaggtggt gttagcaaag catgtatatg agcaggtttt acaaaccctg
     6241 gacaatctcg tgtacagtga agatctgaat aagtatccag ccagtgctac ctcctcccct
     6301 tgccctgatt ctcctctgcc tcccctcagt acctgtggag aatcttctgt tgaaaggaag
     6361 gagaatggat tgttcagcca ctccagcctt tctaacacct ctcagaagtc attgtcagtg
     6421 aaggaagtca aatcctttac tcagattcaa gccacctttt gtatatcaga gcttcaggtt
     6481 cagctaagtg gagatctgac tttgggggcc caaggtcttg tgagcttaaa gtttcaggac
     6541 tttgaggtgg aattcagtaa agaccatccc cagactttat ctattcagat tgccctgcat
     6601 tctctgctga tggaggactt attggagaag aatccagatt ctaaatataa gaacctgatg
     6661 gtgtctcgag gagcccctaa gccatctagt ttagcacaaa aagaatacct ttctcagtct
     6721 tgcccctcag tgtccaatgt ggaatatcct gatatgcctc ggtctctccc ttcccacatg
     6781 gaagaagctc ctaatgtctt ccagttgtat caaaggccca cctctgcgtc ccggaaaaag
     6841 caaaaggaag tccaagacaa ggactatccc ttgaccccac ctccttctcc aacagtggat
     6901 gagcccaaga tacttgttgg aaagagtaaa tttgatgatt ccttagtcca catcaacata
     6961 ttcttggtag ataagaaaca tccagaattc tcttccagtt acaatcgagt taaccggagc
     7021 attgatgttg attttaattg cttggatgtg ctgatcacac tgcaaacctg ggttgtgata
     7081 ttagactttt ttggaatcgg ctccactgca gacaaccacg caatgaggct gcctcctgag
     7141 ggcattctgc acaacgtgaa gttggagcca catgcctcca tggagtctgg acttcaggat
     7201 ccagtgaaca ccaaactgga tctcaaggtt cattcacttt ctctagtgct gaataagacc
     7261 accagtgagc ttgccaaagc aaatgtgtcc aaattagtag cacacctgga aatgattgag
     7321 ggagacctgg ccttacaggg cagcattggg agtctgtctc taagtgacct cacatgccat
     7381 ggagagttct acagagaacg gttcactacc agtggtgaag aagcactcat cttccagact
     7441 tttaaatacg gacggcctga ccctctgctc cggagagaac acgacattcg cgtgagcctc
     7501 cggatggcct ctgtgcagta tgtgcatact cagcgtttcc aggcagaggt ggtggccttc
     7561 attcagcatt tcactcagct gcaggatgtc ttagggcgcc agcgagctgc tattgagggg
     7621 cagacggtga gagatcaagc ccagcgctgt tcacgggttc tcctggatat tgaggctggt
     7681 gctcccgttc tcttgatccc agaaagttcc agatcaaata atctgattgt agcaaatttg
     7741 gggaagttga aagtcaaaaa taagtttctg tttgctggtt ttcctggcac cttttcccta
     7801 caagataagg aatctgtgcc ttcagcttcc ccaacgggta ttcccaaaca cagtctgagg
     7861 aaaacgacaa gcacggagga gcccagggga acccattccc aggggcagtt cacgatgcct
     7921 cttgctggaa tgagcctagg aagcctgaag agtgagtttg tgcccagtac ctccaccaag
     7981 cagcaagggc cgcaacccac actgtctgtt ggccaagagt ccagtagtcc agaagaccat
     8041 gtctgcctgc tggattgcgt tgtcgtggat ctccaggaca tggacatctt tgctgcagag
     8101 agacatccga gagaatactc gaaggcacca gaggatagta gtggagatct gatcttccct
     8161 tcctattttg tgcgacagac aggaggaagc ctcttaaccg agccttgtag gctgaaattg
     8221 caggtggaaa ggaatttgga caaagaaata agtcatactg tgccagacat atctatccat
     8281 ggcaatctct cctcagtcca ctgctctctg gatctgtata aatacaagct gatccgcggc
     8341 ttattagaga acaacctggg agaacccata gaggaattta tgcggcctta tgatttacaa
     8401 gatccaagaa ttcatactgt cctgagtgga gaagtgtaca cctgtatgtg cttcctcatt
     8461 gatatggtga atgtaagtct ggagcttaaa gatccaaaaa gaaaagaagg tgctgggtcc
     8521 ctagccagat ttgacttcaa gaaatgcaaa ctgctctatg aaagtttttc caaccaaacc
     8581 aagtccatta acttggtttc ccattccatg atggcttttg acacccgtta tgctgggcag
     8641 aagaccagcc ctggcatgac gaatgtgttc agctgtatct ttcagcccgc taagaacagc
     8701 agcaccaccc aagggtccat tcagattgaa ctacatttca gatctaccaa ggattcctcc
     8761 tgctttacag tagttctcaa caatctccgt gtgtttctca tatttgactg gctactgtta
     8821 gtccatgatt ttctccacac tcccagtgat attaagaaac aaaatcatgt tactccttct
     8881 cgccaccgta actctagcag cgaatctgct atagttccca aaactgtgaa gagtggagta
     8941 gttaccaagc ggtcttccct tcctgtgtcc aatgaaaggc acctggaggt caaggtcaat
     9001 gtaacaggta cggagtttgt ggtcattgaa gatgtgtcct gcttcgacac caatgccatt
     9061 attctgaaag gcaccacagt gctcacctat aagccccggt ttgttgatcg ccccttttca
     9121 ggaagtttgt ttggcattga ggtgttttca tgccgactag ggaatgagca tgatacagct
     9181 ctttcaattg tggatcccgt acaaattcaa atggagttgg tggggaattc ttcttatcaa
     9241 aatagttcag gattgatgga tgcattcaat agtgaagatt tcccacctgt cctggagatt
     9301 cagttacaag ccctggatat cagactctcc tataatgatg ttcagctgtt tcttgccatt
     9361 gcaaaatcca tcccagagca agctaatgct gcagtgccag actcagtggc cctggagtca
     9421 gactccgttg gcacttacct tccaggtgca tctcgcgttg gagaggaaat cagagaaggg
     9481 acaagacaca ccttagatcc tgtcttggag ttacagctgg ctaggctgca ggagctggga
     9541 ttcagcatgg atgattgtcg caaagctctt ttggcgtgtc aaggccaatt gaaaaaggca
     9601 gcaagttggt tgtttaagaa tgcggaacct ctgaagtctc tttccttggc ctccaccagc
     9661 cgagatagcc caggggctgt ggcagcgcca ttgatctctg gcgtggagat caaagctgag
     9721 agtgtgtgca tctgtttcat cgatgactgc atggattgtg atgttcctct cgctgaactc
     9781 accttttccc gtctgaattt tcttcagcgt gtaagaacta gccctgaagg ctatgcccac
     9841 ttcacccttt ctggagatta ttataaccgt gctctttcag gctgggagcc atttattgag
     9901 ccttggccat gctctgtatc ctggcaacag caggcagcta gtcgtctcca tcctcctcga
     9961 ctgaagctag aagccaaggc caaacctcgt ttggatatca atatcacttc tgtgctaatt
    10021 gaccagtatg taagtaccaa ggaatcgtgg atggcagatt actgtaaaga tgacaaggac
    10081 atagagtcag ctaaatcaga agactggatg ggctcttcgg tggatcctcc atgttttgga
    10141 caaacagagg tgaaaacccc caagcgccgg cagccatttg tcccctttgc tctgaggaac
    10201 cacacggggt gcactttgtg gtttgccacc ctgaccacca cacccaccag agctgcactc
    10261 tctcacagtg ggagtccagg ggtagttcca gaagggaacg gaacatttct cgatgatact
    10321 cacaatgtta gtgaatggcg agaagtcctt acaggtgaag agattccctt tgaatttgaa
    10381 gcaagaggaa agttaagaca cagacacacc catgacctcc ggattcatca actgcaagtg
    10441 agagtaaatg gctgggagca agtgagccca gtgtctgtgg acaaagtcgg gacctttttt
    10501 cgatatgcag caccagataa aaattcatct tcctctacga ttggcagccc aagcagcaga
    10561 acaaatatta tacatcccca ggtttatttc tcttcactcc caccagtgcg ggtggtcttt
    10621 gcagtgacta tggaaggcag tgcacggaaa gtcatcactg tccggtcagc cctcattgtg
    10681 aggaacagac ttgagacacc aatggaacta agactggata gcccatcagc tccagacaag
    10741 ccagtggtgc ttcctgctat catgccaggg gattcgtttg ctgtgccttt acacctcact
    10801 tcttggcggc tacaggcccg gcccaaagga ttgggtgtat ttttctgtaa ggctcccatt
    10861 cattggacca atgtagtgaa gactgcagaa attagtagca gtaaacgaga gtgccactct
    10921 atggacacag aaaaaagccg atttttcagg ttttgtgtgg ctataaagaa agagaattat
    10981 ccagattata tgccctcaaa catattttct gacagtgcaa aacagatttt cagacagcct
    11041 gggcatacca tatatctcct gccaactgtg gtaatctgca acttgctacc ctgtgaactt
    11101 gatttttatg ttaaaggaat gccaattaat gggacgctga aacctggcaa ggaggcagct
    11161 ctccatacag ctgatacatc ccagaacatt gagctggggg tatcactgga gaatttcccc
    11221 ctctgtaaag aattgctcat tccacctgga acccaaaact atatggtgag aatgcgactc
    11281 tatgacgtca accgtcggca gctgaacctc accatccgga ttgtgtgtcg agcagaagga
    11341 tccttaaaga tcttcatttc tgctccatat tggctgatta acaaaacagg gttgccactg
    11401 atcttcagac aggacaatgc caagacagat gctgcaggcc agtttgagga gcatgagctg
    11461 gcccgtagcc tgagtcctct cttattctgc tatgctgaca aagagcagcc aaacctctgc
    11521 acgatgagaa tcggaagggg gattcatcca gaaggcatgc cgggctggtg tcagggcttc
    11581 tccctggatg gtggtagtgg tgtccgagct ttgaaagtca tccagcaagg aaaccgccca
    11641 gggctgatct ataacattgg tattgatgtc aagaaaggcc gaggtcgata cattgatacc
    11701 tgcatggtca tctttgcccc ccgttacctg ttagataata aatcatctca caagcttgca
    11761 tttgcacaga gggaatttgc caggggacag ggaacagcca atcccgaagg ttacatttcc
    11821 acccttcctg gttccagtgt ggtgttccac tggcctcgga atgactatga tcagctattg
    11881 tgtgtcagac tgatggacgt tcccaattgt atttggtctg gaggctttga agtcaacaag
    11941 aataattcct tccatatcaa catgagggat accttgggaa aatgcttctt cctacgagtg
    12001 gaaattactc tccgaggagc tacgtatagg atctcattta gtgacacaga tcagttacct
    12061 cctcctttcc gaattgacaa cttttctaag gtcccggttg tctttactca gcatggcgta
    12121 gctgaaccca ggctccggac tgaagtgaag cccatgactt cattggatta tgcctgggac
    12181 gaacccacct tgccaccttt tatcactctg actgttaaag gggcagggtc ctctgagatc
    12241 aactgcaaca tgaatgattt ccaggataat cggcagcttt attatgaaaa tttcatttac
    12301 attgctgcta catatacatt ctctggcttg caggagggaa caggcaggcc tgtggcttcc
    12361 aacaaggcca ttacctgtgc ggagctcgtt ttggatgtct cacccaagac acaaagagtc
    12421 attttaaaaa agaaggaacc aggaaagcgt tctcagctgt ggaggatgac aggaacagga
    12481 atgctggccc atgagggctc ctcagttcct cacaatccca ataagccctc agccgcccgc
    12541 tccaccgagg ggtctgccat cttagatatt gctggtctcg ctgcagtgac tgacaacaga
    12601 tacgagccac tgatgctgag aaagcctgac cgcaggcgaa gcacaactca gacgtggagt
    12661 ttccgagaag gaaaactgac ctgtgggtta catgggttgg tcgtccaggc caaaggagga
    12721 ctttctggtt tgtttgatgg agctgaagtt gttcttggtc ctgacacttc catggagctt
    12781 ttggggccag ttccacctga acaacaattt attaatcaaa aaatgagacc tggttctgga
    12841 atgttatcca tcagagtcat cccagatgga ccaactagag cactccagat aacagatttc
    12901 tgccaccgga aaagcagccg ttcatatgaa gtggatgaac ttcctgtcac cgaacaagag
    12961 ctgcagaaat taaagaatcc agatacagag caggaattgg aagtgcttgt gaggttagaa
    13021 ggtggaattg ggttgtcctt aattaataaa gtcccagaag aactggtctt tgcaagtctt
    13081 acaggaatca atgtgcacta tacacagctg gcaaccagtc acatgcttga actcagcata
    13141 caggatgtac aggtggacaa tcagctcatt ggtaccacgc agcccttcat gctctatgtg
    13201 actcccctga gcaatgagaa tgaggtcatc gagaccggcc cagctgtgca agtcaacgca
    13261 gtgaagttcc ccagtaagag tgcactgacc aacatctaca agcatctgat gatcacagct
    13321 cagagattca cagtgcaaat tgaggagaaa ctgctcctca agctgctaag tttctttggc
    13381 tacgatcaag cagaatcaga ggtggaaaaa tatgatgaaa acctccatga aaagacagct
    13441 gagcaaggtg gaacaccaat tcgatactac tttgaaaatc tcaaaatcag cattcctcag
    13501 atcaagctaa gtgtgttcac ctccaacaag ctcccattgg atcttaaggc cctaaaaagc
    13561 accttggggt ttcctttgat acggtttgaa gacgctgtga ttaatctaga tccattcact
    13621 cgggtacatc cctatgagac caaggagttc atcatcaatg atatcctcaa acatttccag
    13681 gaggaactcc tcagccaggc agctcgaatc ctgggatcag tggattttct tggcaatcct
    13741 atggggcttt tgaatgatgt ttctgaaggg gttactggac tgataaaata tggaaatgtc
    13801 gggggcctca tcagaaatgt tacacacgga gtatcaaact ctgctgccaa gtttgctgga
    13861 acattatcag atggcttagg gaagacgatg gacaatcggc atcagtcaga gcgggagtac
    13921 atcaggtacc atgcagccac aagtggtgaa caccttgtag ccggcatcca tggcctggct
    13981 catggtatca ttggtggact gaccagtgtt ataacttcga cagtggaagg tgtgaaaaca
    14041 gaagggggtg tcagcggttt catatctggc cttggaaaag ggcttgttgg cactgtaacc
    14101 aagccagtgg caggcgccct ggattttgca tcagaaacag cccaggcggt gagagacaca
    14161 gccacactca gcggccccag gactcaagca cagagggttc ggaaaccgcg ttgctgcacg
    14221 gggccccagg ggctgcttcc ccgatattct gagagccagg cggaaggaca ggagcagctc
    14281 ttcaaactca cagacaacat acaggacgaa ttcttcatcg ctgtggagaa cattgacagc
    14341 tactgcgtgc tcatctcctc caaagctgtt tacttcctga aaagtggaga ctacgtggat
    14401 cgagaagcca ttttcctaga agtcaaatac gatgacctct accactgcct tgtctccaaa
    14461 gaccatggga aggtgtatgt gcaggtgacc aagaaagccg tgagcacgag cagtggagtg
    14521 tccatccccg gcccctccca ccagaagccc atggtccatg tgaaatctga ggtccttgct
    14581 gtcaagttgt cacaagaaat aaactacgca aagagcctct actatgaaca gcagcttatg
    14641 ttaagactca gcgaaaaccg agagcagctg gagctggact cctgatctag ataactgatc
    14701 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    14761 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    14821 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    14881 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
    14941 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
    15001 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
    15061 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
    15121 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
    15181 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    15241 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    15301 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
    15361 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
    15421 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    15481 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
    15541 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    15601 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    15661 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    15721 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    15781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    15841 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
    15901 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
    15961 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
    16021 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
    16081 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
    16141 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
    16201 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
    16261 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
    16321 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
    16381 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
    16441 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
    16501 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
    16561 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
    16621 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
    16681 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
    16741 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    16801 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    16861 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
    16921 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
    16981 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
    17041 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
    17101 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
    17161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    17221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    17281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    17341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    17401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    17461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    17521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    17581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    17641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    17701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    17761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    17821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    17881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    17941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    18001 attaccgcca tgcat
//
