LOCUS       sequence-356912-        6164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of C-terminal deleted mouse Armc12 tagged with 
            FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. 
            Acad. Sci. USA. 118 (6): e2018355118, 2021..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, Matzuk MM, 
            Ikawa M
  TITLE     ARMC12 regulates spatiotemporal mitochondrial dynamics during 
            spermiogenesis and is required for male fertility.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Feb 9;118(6). pii: 2018355118. doi: 
            10.1073/pnas.2018355118.
  PUBMED    33536340
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-02-9"; volume: "118(6). pii: 
            2018355118. doi"; pages: " 10.1073/pnas.2018355118"
COMMENT     Sequence Label: pCAG1.1-Armc12 delta C/FLAG
FEATURES             Location/Qualifiers
     source          1..6164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          675..1689
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1615..1637
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1697..1716
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1756..1765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2734..2757
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2844..2863)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2909..2964
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2963..2982)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3319..3338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3320..3338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3348..3365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3348..3364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3357..3379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3506..3961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3593..3612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3803..3824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3987..4091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4092..4160,4161..4952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4310..4329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5123..5711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5612..5631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5865..5882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5999..6020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6035..6040,6041..6058,6059..6065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6073..6089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6078..6100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6097..6113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6097..6113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6132..6152
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6134..6152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggncg cgcgccrggs ggggsggggs
      541 ggggsgrggg gsggggsggg gsgrggcggr gaggkgcggc ggcagccaat cagagcggcg
      601 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      661 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      721 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      781 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
      841 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
      901 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      961 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1021 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1081 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1141 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1201 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1261 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     1321 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1381 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1441 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1501 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1561 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1621 gcttctggcg tgtgaccggc ggctctagcc tctgctaacc atgttcatgc cttcttcttt
     1681 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaatct
     1741 agaggatccc tgcaggccgc catgggcaag accattcccc ggttcctgga gcaactggac
     1801 ctcatcaaaa gcttcgtggg cctggctaca ggcgccgggg ccttatacct gctgtacaag
     1861 gccgtcagga ctggcttaaa atgtcatccg ccactctgct ccaactcgcc gatctgcatt
     1921 gcccgcctgg ccatcgagag agagcgccac gggcgggact ccggtgagat tcgaagactt
     1981 ctcaactctc tagactgcaa acaggatgag tataccagga gcatgatcct tcataacatc
     2041 acccgctgcg tgtacttgct ggaggctgag gcctcttctt gtactatgga tgacatcgac
     2101 ttggtggctg acatgctaga tgagaaggac aacagtgtaa aaatccaagc tctgaatgca
     2161 cttaaagctt tctctggcat caggaaattc aggctcaaaa tccaggagca ttgcatcaag
     2221 gtactggaac tgatttccac catctgggac ttggaattgc acgtagctgg tctccgattg
     2281 ctcaacaacc tcccgctacc tgactatgtg cacccacagc tgcggcgggt gatgcctgcc
     2341 ctgatggaga tcatacagtc agactgcatc ctggcacagg tacaagctgt ccgcctcctg
     2401 agttacctgg cacagaagaa cgaccttctt tatgacattc tcaactgtca ggtgcgtccc
     2461 aacttcctga acctcttcca gtcttcacag ccgggcagtc tgctgttcga ggtgctggtg
     2521 tttgcggaac acttgagtga aggtcggaac gccacccact accgagcggt caaatggcat
     2581 tacaacgagc aatccctgca cgaagccctc tttggggatg agtccagact ggcagaccga
     2641 ctgctctccc tggtcatcca ccctgaggag gaagttcaga tccaggcctg caaagtcata
     2701 gtgagcttgc agtgtcccgg cggcagcgat atcgactaca aggatgacga tgacaagtag
     2761 ctcgagactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     2821 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     2881 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     2941 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     3001 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     3061 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     3121 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     3181 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     3241 ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc cctcgactta
     3301 attaaggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3481 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3901 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     3961 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6121 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctg
//
