LOCUS       sequence-356913-        6227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of mouse armadillo repeat containing 12 (Armc12) 
            tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. 
            Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, Matzuk MM, 
            Ikawa M
  TITLE     ARMC12 regulates spatiotemporal mitochondrial dynamics during 
            spermiogenesis and is required for male fertility.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Feb 9;118(6). pii: 2018355118. doi: 
            10.1073/pnas.2018355118.
  PUBMED    33536340
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-02-9"; volume: "118(6). pii: 
            2018355118. doi"; pages: " 10.1073/pnas.2018355118"
COMMENT     Sequence Label: pCAG1.1-Armc12/FLAG
FEATURES             Location/Qualifiers
     source          1..6227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          675..1689
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1615..1637
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1697..1716
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1756..1765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2797..2820
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2907..2926)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2972..3027
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3026..3045)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3382..3401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3383..3401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3411..3428)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3411..3427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3420..3442)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3569..4024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3656..3675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3866..3887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4050..4154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4155..4223,4224..5015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4373..4392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5186..5774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5675..5694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5928..5945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6062..6083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6098..6103,6104..6121,6122..6128)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6136..6152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6141..6163
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6160..6176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6160..6176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6195..6215
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6197..6215
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggnsg cgcgccrggs ggggsggggs
      541 ggggsgrggg gsggggsggg gsgrggcggr gaggtgcggc ggcagccaat cagagcggcg
      601 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      661 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      721 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      781 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
      841 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
      901 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      961 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1021 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1081 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1141 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1201 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1261 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     1321 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1381 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1441 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1501 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1561 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1621 gcttctggcg tgtgaccggc ggctctagcc tctgctaacc atgttcatgc cttcttcttt
     1681 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaatct
     1741 agaggatccc tgcaggccgc catgggcaag accattcccc ggttcctgga gcaactggac
     1801 ctcatcaaaa gcttcgtggg cctggctaca ggcgccgggg ccttatacct gctgtacaag
     1861 gccgtcagga ctggcttaaa atgtcatccg ccactctgct ccaactcgcc gatctgcatt
     1921 gcccgcctgg ccatcgagag agagcgccac gggcgggact ccggtgagat tcgaagactt
     1981 ctcaactctc tagactgcaa acaggatgag tataccagga gcatgatcct tcataacatc
     2041 acccgctgcg tgtacttgct ggaggctgag gcctcttctt gtactatgga tgacatcgac
     2101 ttggtggctg acatgctaga tgagaaggac aacagtgtaa aaatccaagc tctgaatgca
     2161 cttaaagctt tctctggcat caggaaattc aggctcaaaa tccaggagca ttgcatcaag
     2221 gtactggaac tgatttccac catctgggac ttggaattgc acgtagctgg tctccgattg
     2281 ctcaacaacc tcccgctacc tgactatgtg cacccacagc tgcggcgggt gatgcctgcc
     2341 ctgatggaga tcatacagtc agactgcatc ctggcacagg tacaagctgt ccgcctcctg
     2401 agttacctgg cacagaagaa cgaccttctt tatgacattc tcaactgtca ggtgcgtccc
     2461 aacttcctga acctcttcca gtcttcacag ccgggcagtc tgctgttcga ggtgctggtg
     2521 tttgcggaac acttgagtga aggtcggaac gccacccact accgagcggt caaatggcat
     2581 tacaacgagc aatccctgca cgaagccctc tttggggatg agtccagact ggcagaccga
     2641 ctgctctccc tggtcatcca ccctgaggag gaagttcaga tccaggcctg caaagtcata
     2701 gtgagcttgc agtgtcccca ggacctggga tcccgaccct cttcctgccg acctagccat
     2761 tcctgcttta aaaccgggaa gggcggcagc gatatcgact acaaggatga cgatgacaag
     2821 tagctcgaga ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     2881 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     2941 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3001 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3061 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     3121 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     3181 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     3241 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     3301 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac
     3361 ttaattaagg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     3421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctg
//
