LOCUS       sequence-356915-        4740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC15A2_STOP
FEATURES             Location/Qualifiers
     source          1..4740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2297..2396)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2413..2432)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2414..2432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2437..2453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2437..2453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2555..2574)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2566..3375
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2633..2652)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3522..4110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4011..4030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4264..4281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4440..4467)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4559..4645)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4694..4716
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4708..4725
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4709..4725
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa ccctttccag
      121 aagaatgaga gcaaggagac cctgttttct ccagtgagca tcgaggaggt gccacctagg
      181 ccaccatctc ctccaaagaa gcccagccct acaatctgcg gctctaacta cccactgagc
      241 atcgccttca tcgtggtgaa tgagttctgt gagcggttca gctactatgg catgaaggcc
      301 gtgctgatcc tgtatttcct gtactttctg cactggaacg aggatacctc tacaagcatc
      361 tatcacgcct tcagctccct gtgctacttt accccaatcc tgggagcagc aatcgcagac
      421 tcttggctgg gcaagttcaa gacaatcatc tatctgtctc tggtgtacgt gctgggccac
      481 gtgatcaaga gcctgggcgc cctgcctatc ctgggaggac aggtggtgca caccgtgctg
      541 tccctgatcg gcctgtctct gatcgccctg ggcacaggcg gcatcaagcc atgcgtggca
      601 gccttcggcg gcgatcagtt tgaggagaag cacgccgagg agcggaccag atatttcagc
      661 gtgttttacc tgtccatcaa cgccggctcc ctgatctcta ccttcatcac accaatgctg
      721 aggggcgacg tgcagtgctt cggcgaggat tgttatgcac tggcctttgg cgtgccagga
      781 ctgctgatgg tcatcgccct ggtggtgttc gccatgggct ccaagatcta caacaagccc
      841 cctccagagg gcaatatcgt ggcccaggtg ttcaagtgca tctggtttgc catcagcaac
      901 cggtttaaga atagatccgg cgatatcccc aagaggcagc actggctgga ctgggcagca
      961 gagaagtatc ctaagcagct gatcatggat gtgaaggccc tgacccgggt gctgttcctg
     1021 tacatcccac tgcccatgtt ttgggccctg ctggaccagc agggcagcag atggacactg
     1081 caggccatca ggatgaaccg caatctgggc ttctttgtgc tgcagccaga tcagatgcag
     1141 gtgctgaacc ccctgctggt gctgatcttc atccctctgt tcgactttgt gatctacagg
     1201 ctggtgtcca agtgcggcat caatttctct agcctgcgca agatggccgt gggcatgatc
     1261 ctggcatgtc tggcctttgc cgtggcagca gccgtggaga tcaagatcaa cgagatggca
     1321 ccagcacagc ctggaccaca ggaggtgttc ctgcaggtgc tgaacctggc cgacgatgag
     1381 gtgaaggtga ccgtggtggg caatgagaac aatagcctgc tgatcgagag catcaagtcc
     1441 tttcagaaga cccctcacta ttctaagctg cacctgaaga caaagagcca ggatttccac
     1501 tttcacctga agtatcacaa tctgtccctg tacacagagc actctgtgca ggagaagaac
     1561 tggtactccc tggtcatccg ggaggatggc aattctatct cctctatgat ggtgaaggac
     1621 accgagtccc ggaccacaaa cggcatgacc acagtgagat tcgtgaacac actgcacaag
     1681 gacgtgaata tctccctgtc taccgataca agcctgaatg tgggcgagga ctatggcgtg
     1741 tccgcctaca ggaccgtgca gaggggagag tatccagcag tgcactgtag aacagaggat
     1801 aagaactttt ctctgaatct gggactgctg gacttcggag cagcatacct gtttgtgatc
     1861 accaacaata caaaccaggg cctgcaggcc tggaagatcg aggacatccc agccaataag
     1921 atgagcatcg cctggcagct gccacagtat gcactggtga ccgcaggcga agtgatgttc
     1981 agcgtgacag gcctggagtt tagctactcc caggccccta gctccatgaa gtccgtgctg
     2041 caggcagcat ggctgctgac catcgcagtg ggcaacatca tcgtgctggt ggtggcacag
     2101 ttcagcggac tggtgcagtg ggccgagttc atcctgtttt cctgcctgct gctggtcatc
     2161 tgtctgatct tttctatcat gggctactat tacgtgccag tgaagaccga ggacatgagg
     2221 ggaccagcag ataagcacat ccctcacatc cagggcaata tgatcaagct ggagaccaag
     2281 aagacaaagc tgtaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     2341 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     2401 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     2461 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2521 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2581 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2641 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2701 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2761 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2821 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2881 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2941 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     3001 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     3061 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     3121 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     3181 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     3241 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     3301 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3361 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     3421 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4141 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4201 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4261 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4321 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4381 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4441 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4501 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4561 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4621 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4681 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
