LOCUS       sequence-356917-        5570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of ARM domain deleted mouse Armc12 tagged with 
            FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. 
            Acad. Sci. USA. 118 (6): e2018355118, 2021..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, Matzuk MM, 
            Ikawa M
  TITLE     ARMC12 regulates spatiotemporal mitochondrial dynamics during 
            spermiogenesis and is required for male fertility.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Feb 9;118(6). pii: 2018355118. doi: 
            10.1073/pnas.2018355118.
  PUBMED    33536340
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-02-9"; volume: "118(6). pii: 
            2018355118. doi"; pages: " 10.1073/pnas.2018355118"
COMMENT     Sequence Label: pCAG1.1-Armc12 delta ARM/FLAG
FEATURES             Location/Qualifiers
     source          1..5570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          675..1689
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1615..1637
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1697..1716
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1756..1765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2140..2163
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2250..2269)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2315..2370
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2369..2388)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2725..2744)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2726..2744)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2754..2771)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2754..2770)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2763..2785)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2912..3367
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2999..3018)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3209..3230
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3393..3497
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3498..3566,3567..4358)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3716..3735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4529..5117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5018..5037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5271..5288
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5405..5426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5441..5446,5447..5464,5465..5471)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5479..5495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5484..5506
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5503..5519
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5503..5519
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5538..5558
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5540..5558
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggnsg cgcgccrggs ggggsggggs
      541 ggggsggggg gsggggsggg gsgrggcggr gaggtgcggc ggcagccaat cagagcggcg
      601 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      661 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      721 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      781 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
      841 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
      901 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      961 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1021 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1081 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1141 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1201 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1261 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     1321 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1381 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1441 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1501 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1561 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1621 gcttctggcg tgtgaccggc ggctctagcc tctgctaacc atgttcatgc cttcttcttt
     1681 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaatct
     1741 agaggatccc tgcaggccgc catgggcaag accattcccc ggttcctgga gcaactggac
     1801 ctcatcaaaa gcttcgtggg cctggctaca ggcgccgggg ccttatacct gctgtacaag
     1861 gccgtcagga ctggcttaaa atgtcatccg ccactctgct ccaactcgcc gatctgcatt
     1921 gcccgcctgg ccatcgagag agagcgccac gggcgggact ccggtgagat tcgaagactt
     1981 ctcaactctc tagactgcaa acaggatgag tataccagga gcatgatcct tcataacatc
     2041 acccgctgcg tgtacttgct gcaggacctg ggatcccgac cctcttcctg ccgacctagc
     2101 cattcctgct ttaaaaccgg gaagggcggc agcgatatcg actacaagga tgacgatgac
     2161 aagtagctcg agactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     2221 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     2281 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     2341 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     2401 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     2461 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     2521 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     2581 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2641 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg aagatccctc
     2701 gacttaatta aggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     2761 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2821 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2881 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     2941 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3001 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3061 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3121 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3181 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3241 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3301 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3361 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3421 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3481 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3541 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3601 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3661 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3721 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3781 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3841 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3901 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3961 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4021 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4081 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4141 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4201 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4261 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4321 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4381 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4441 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4501 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4561 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4621 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4681 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4741 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4801 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4861 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4921 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4981 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5041 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5101 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5161 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5221 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5281 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5341 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5401 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5461 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5521 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
//
