LOCUS       sequence-356920-        4284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC15A4_STOP
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1841..1940)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1957..1976)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1958..1976)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1981..1997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1981..1997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2099..2118)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2110..2919
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2177..2196)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3066..3654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3555..3574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3808..3825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3984..4011)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4103..4189)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4238..4260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4252..4269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4253..4269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gggcagcggc
      121 ggcggcgccg gcgagagggc ccccctgctg ggcgcccgga gagccgccgc agcagcagca
      181 gcagcaggag cctttgccgg caggcgcgcc gcctgcggag ccgtgctgct gacagagctg
      241 ctggagaggg cagcctttta tggcattaca tccaacctgg tactgttcct gaatggcgcc
      301 ccattttgtt gggagggagc acaggcaagc gaggccctgc tgctgttcat gggcctgaca
      361 tacctgggct ccccctttgg cggctggctg gcagacgcaa ggctgggaag ggcaagggcc
      421 atcctgctga gcctggccct gtatctgctg ggaatgctgg catttccact gctggcagca
      481 cctgcaacaa gggccgccct gtgcggctct gccagactgc tgaactgtac cgcaccagga
      541 ccagatgcag cagcaaggtg ctgtagccca gccacattcg ccggactggt gctggtggga
      601 ctgggagtgg caacagtgaa ggccaatatc accccctttg gcgccgacca ggtgaaggat
      661 agaggccctg aggccacccg gagattcttt aactggttct actggtctat caatctggga
      721 gcaatcctga gcctgggagg aatcgcctac atccagcaga acgtgtcctt tgtgacaggc
      781 tatgcaatcc caaccgtgtg cgtgggactg gccttcgtgg tgttcctgtg cggccagtcc
      841 gtgttcatca ccaagccccc tgacggctct gccttcacag atatgtttaa gatcctgacc
      901 tatagctgct gttcccagaa gaggtccggc gagcgccagt ctaatggcga gggcatcggc
      961 gtgttccagc agagctccaa gcagtccctg tttgactctt gcaagatgag ccacggcggc
     1021 cccttcacag aggagaaggt ggaggatgtg aaggccctgg tgaagatcgt gccagtgttc
     1081 ctggccctga tcccctactg gaccgtgtat tttcagatgc agaccacata cgtgctgcag
     1141 agcctgcacc tgcggatccc tgagatctcc aacatcacca caacccctca cacactgcca
     1201 gccgcctggc tgaccatgtt tgacgccgtg ctgatcctgc tgctgatccc tctgaaggac
     1261 aagctggtgg atccaatcct gaggcgccac ggcctgctgc cttctagcct gaagaggatc
     1321 gccgtgggca tgttctttgt gatgtgctct gccttcgccg caggaatcct ggagagcaag
     1381 cgcctgaatc tggtgaagga gaagacaatc aaccagacca tcggcaatgt ggtgtaccac
     1441 gccgccgatc tgtccctgtg gtggcaggtg cctcagtatc tgctgatcgg catcagcgag
     1501 atcttcgcct ccatcgccgg cctggagttt gcctattctg ccgccccaaa gtctatgcag
     1561 agcgccatca tgggcctgtt ctttttcttt tccggagtgg gatcttttgt gggaagcgga
     1621 ctgctggccc tggtgagcat caaggccatc ggctggatgt cctctcacac agacttcggc
     1681 aacatcaatg gctgctacct gaactactat ttctttctgc tggcagcaat ccagggagcc
     1741 accctgctgc tgttcctgat catctccgtg aagtatgacc accacagaga tcaccagcgg
     1801 tccagagcca atggcgtgcc aacctctcgg agagcctaag acccagcttt cttgtacaaa
     1861 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1921 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1981 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2041 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2101 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2161 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2221 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2281 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2341 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2401 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2461 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2521 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2581 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2641 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2701 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2761 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2821 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2881 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2941 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     3001 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3061 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3181 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3721 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3781 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3841 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3901 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3961 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4021 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4081 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4141 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4201 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4261 acggccagtc ttaagctcgg gccc
//
