LOCUS       sequence-356921-        4656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A1_STOP
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2213..2312)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2329..2348)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2330..2348)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2353..2369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2353..2369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2471..2490)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2482..3291
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2549..2568)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3438..4026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3927..3946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4180..4197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4356..4383)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4475..4561)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4610..4632
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4624..4641
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4625..4641
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cgagtcccct
      121 gagccactgc agcagggaag aggaccagtg cctgtgcgga gacagaggcc agcaccaaga
      181 ggactgaggg agatgctgaa ggccagactg tggtgcagct gttcctgcag cgtgctgtgc
      241 gtgcgggccc tggtgcagga cctgctgcca gccacccgct ggctgcggca gtacaggcct
      301 cgcgagtatc tggccggcga tgtgatgagc ggcctggtca tcggcatcat cctggtgcca
      361 caggccatcg catattccct gctggcagga ctgcagccca tttactcctt gtatacgtca
      421 ttctttgcca acctgatcta cttcctgatg ggcacctctc gccacgtgag cgtgggcatc
      481 tttagcctgc tgtgcctgat ggtgggccag gtggtggaca gggagctgca gctggcaggc
      541 ttcgacccat ctcaggatgg actgcagcct ggagccaaca gctccacact gaatggcagc
      601 gccgccatgc tggactgtgg cagagattgc tacgccatca gggtggccac cgccctgaca
      661 ctgatgaccg gcctgtatca ggtgctgatg ggcgtgctga ggctgggatt cgtgtccgcc
      721 tacctgtctc agcctctgct ggatggcttt gccatgggcg cctctgtgac aatcctgacc
      781 agccagctga agcacctgct gggcgtgaga atccctaggc accagggccc aggcatggtg
      841 gtgctgacct ggctgtccct gctgagggga gcaggacagg ccaacgtgtg cgacgtggtg
      901 acctccacag tgtgcctggc cgtgctgctg gcagcaaagg agctgtctga tcggtataga
      961 cacaggctgc gcgtgcctct gccaacagag ctgctggtca tcgtggtggc caccctggtg
     1021 agccacttcg gccagctgca caagagattt ggctctagcg tggccggcga catccccaca
     1081 ggcttcatgc cacctcaggt gccagagcct aggctgatgc agagggtggc cctggatgca
     1141 gtggccctgg ccctggtggc agcagccttc agcatctccc tggccgagat gtttgcacgc
     1201 agccacggat actccgtgcg ggcaaaccag gagctgctgg cagtgggctg ctgtaatgtg
     1261 ctgccagcct tcctgcactg ttttgccacc tccgccgccc tggcaaagtc tctggtgaag
     1321 accgccacag gctgcagaac acagctgtcc tctgtggtgt ccgccaccgt ggtgctgctg
     1381 gtgctgctgg ccctggcacc cctgtttcac gacctgcaga ggtctgtgct ggcctgcgtg
     1441 atcgtggtga gcctgagagg cgccctgagg aaagtgtggg acctgccacg cctgtggcgg
     1501 atgtccccag cagatgcact ggtgtgggca ggcacagccg ccacctgcat gctggtgtct
     1561 accgaggcag gactgctggc aggcgtgatc ctgtctctgc tgagcctggc aggaaggaca
     1621 cagaggccca gaaccgccct gctggcccgg atcggcgaca cagccttcta tgaggatgcc
     1681 accgagtttg agggactggt gccagagcca ggcgtgagag tgttcaggtt tggcggccct
     1741 ctgtactatg ccaacaagga cttctttctg cagtccctgt actctctgac aggactggat
     1801 gcaggatgta tggcagcaag gcgcaaggag ggcggcagcg agaccggagt gggagaggga
     1861 ggacctgcac agggagagga tctgggacca gtgtccacaa gggccgccct ggtgcctgca
     1921 gcagcaggat tccacaccgt ggtcatcgac tgcgccccac tgctgtttct ggatgcagca
     1981 ggcgtgagca cactgcagga cctgcggaga gattacggcg ccctgggaat cagcctgctg
     2041 ctggcatgct gttccccacc cgtgcgcgac atcctgtcta ggggaggctt cctgggagag
     2101 ggaccaggcg acaccgccga ggaggagcag ctgtttctga gcgtgcacga tgccgtgcag
     2161 acagccaggg cccgccaccg ggagctggag gccaccgatg cccacctgta agacccagct
     2221 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2281 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     2341 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2401 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     2461 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2521 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2581 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2641 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2701 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2761 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2821 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2881 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2941 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3001 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     3061 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3121 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3181 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3241 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3301 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3361 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3421 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3481 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3541 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3601 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3661 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3721 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3781 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3841 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3901 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3961 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4021 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4081 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4141 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4201 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4261 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     4321 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     4381 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     4441 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     4501 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     4561 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     4621 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
