LOCUS       sequence-356930-       13223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AtTIR1(F79G) in C elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa
            H, Hayashi KI, Harata M, Kanemaki MT
  TITLE     The auxin-inducible degron 2 (AID2) system enables controlled 
            protein knockdown during embryogenesis and development in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218.
  PUBMED    34865044
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218."
COMMENT     Sequence Label: pTN14
FEATURES             Location/Qualifiers
     source          1..13223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1605..2204
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(4893..4926)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5947..5971
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6004..6032)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6780..6800
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7862..7878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8700..9494
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8754..8773)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9364..9383
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(10224..10257)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(11035..11053)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11074..11090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11074..11090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11087..11109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11098..11114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11122..11128,11129..11146,11147..11152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11167..11188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11305..11322)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11476..12064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11556..11575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12235..13026,13027..13095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12858..12877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13096..13200)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctgggaat
      661 gtgcgtggtg tttgtaaaat cgtaataatc acataaatac aatcaactca aaatgatgaa
      721 accagcaaaa atagaaacaa gaaaatgcga ttcaagtgaa tagggcaaca ttcctcttca
      781 tctcattctt caactaaatt taatttggct tccagttttt tctctattct tttccgtctt
      841 ctttcgttcc ttttccaatt gcactttttg taccataggg accctccgtc ttcgtttttt
      901 tctgcaaaca aaagcgccac cctaactatc attccgcttt acaaaggaaa tgagaagaac
      961 gaactgggag agcccagggt ggccttcttc ttcgtttggt tttttaagag tctcgggtcc
     1021 cgagggtcca ttcaaaattg agaacatgct gttttgtaga aaacgaaagg cgacattccg
     1081 caagatttgg gtggtctccg acgagagacg tttagcccga ttgcctgatt tatgaccatg
     1141 tgcactttta cagacttgtt tagtgtcaat atgaaaaacg aatgtgcaaa actggttgat
     1201 gaaaggcaag cgtgaactag tacgtgtgaa tctgacatct aaagttgaat tataaaaaac
     1261 tcgtagggaa aatacggtaa tccatttatc agactcaatt caaataaata ttctgaaaca
     1321 cagtatcaga gttcataatc tgatgttgca atgttatcag tgaattcaat tagttagaat
     1381 gtttcgccga taaccctatt tagttttctt tcccatttca gcggagaata attatttttt
     1441 cattcagtgt ttcaatttat ttttattttt cagaaaatgg gtattatttc cattattttc
     1501 cttcttattg ggtcttcgac ggctgcagtc ctcgaacatc aattaatatg gaaagaatct
     1561 agaatgattg aaatgatcag aaatggagaa tatcctgcct cgaggcacct ttggtctttt
     1621 attgtcaact tccattggtt cttccattgt ttctgttaaa ttaatgaatt tttcataaaa
     1681 taaagacatt atacaatata aaaatgaaga atttattgaa aataaactgc cagagagaaa
     1741 aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt acggtacatt ttcgtgctag
     1801 gagttagatg tgcaggcagc aacgagaggg ggagagattt ttttgggcct tgtgaaatta
     1861 acgtgagttt tctggtcatc tgactaatca tgttggtttt ttgttggttt attttgtttt
     1921 tatctttgtt tttatccaga ttaggaaatt taaattttat gaatttataa tgaggtcaaa
     1981 cattcagtcc cagcgttttt cctgttctca ctgtttagtc gaatttttat tttaggcttt
     2041 caacaaatgt tctaactgtc ttatttgtga cctcactttt tatatttttt taatttttaa
     2101 aaatattaga agtttctagg ataatttttt cgacttttat tctctctacc gtccgcactc
     2161 ttcttacttt taaattaaat tgtttttttt tcagttggga aacactttgc tcatgcaaaa
     2221 gagaatcgcc ttgtcgtttc cagaagaagt actcgagcac gtcttctcct tcatccaact
     2281 cgacaaggac cgtaactccg tctccctcgt ctgcaagtcc tggtacgaga tcgagcgttg
     2341 gtgccgtcgt aaggtcttca tcggaaactg ctacgccgtc tccccagcca ccgtcatccg
     2401 tcgtttccca aaggtccgtt ccgtcgagct caagggaaag ccacacggcg ccgacttcaa
     2461 cctcgtccca gacggatggg gaggatacgt ctacccatgg atcgaggcca tgtcctcctc
     2521 ctacacctgg ctcgaggaga tccgtctcaa gcgtatggtc gtcaccgacg actgcctcga
     2581 gctcatcgcc aagtccttca agaacttcaa ggtcctcgtc ctctcctcct gcgagggatt
     2641 ctccaccgac ggactcgccg ccatcgccgc cacctgccgt aacctcaagg taagtttaaa
     2701 catatatata ctaactaacc ctgattattt aaattttcag gagctcgacc tccgtgagtc
     2761 cgacgtcgac gaggtctccg gacactggct ctcccacttc ccagacacct acacctccct
     2821 cgtctccctc aacatctcct gcctcgcctc cgaggtctcc ttctccgccc tcgagcgtct
     2881 cgtcacccgt tgcccaaacc tcaagtccct caagctcaac cgtgccgtcc cactcgagaa
     2941 gctcgccacc ctcctccaac gtgccccaca actcgaggag ctcggaaccg gaggatacac
     3001 cgccgaggtc cgtccagacg tctactccgg actctccgtc gccctctccg gatgcaaggt
     3061 aagtttaaac agttcggtac taactaacca tacatattta aattttcagg agctccgttg
     3121 cctctccgga ttctgggacg ccgtcccagc ctacctccca gccgtctact ccgtctgctc
     3181 ccgtctcacc accctcaacc tctcctacgc caccgtccaa tcctacgacc tcgtcaagct
     3241 cctctgccaa tgcccaaagc tccaacgtct ctgggtcctc gactacatcg aggacgccgg
     3301 actcgaggtc ctcgcctcca cctgcaagga cctccgtgag ctccgtgtct tcccatccga
     3361 gccattcgtc atggagccaa acgtcgccct caccgagcaa ggactcgtct ccgtctccat
     3421 gggatgccca aagctcgagt ccgtcctcta cttctgccgt caaatgacca acgccgccct
     3481 catcaccatc gcccgtaacc gtccaaacat gacccgtttc cgtctctgca tcatcgagcc
     3541 aaaggcccca gactacctca ccctcgagcc actcgacatc ggattcggag ccatcgtcga
     3601 gcactgcaag gacctccgtc gtctctccct ctccggactc ctcaccgaca aggtcttcga
     3661 gtacatcgga acctacgcca agaagatgga gatgctctcc gtcgccttcg ccggagactc
     3721 cgacctcgga ctccaccacg tcctctccgg atgcgactcc ctccgtaagc tcgagatccg
     3781 tgactgccca ttcggagaca aggccctcct cgccaacgcc tccaagctcg agaccatgcg
     3841 ttccctctgg atgtcctcct gctccgtctc cttcggagcc tgcaagctcc tcggacaaaa
     3901 gatgccaaag ctcaacgtcg aggtcatcga cgagcgtgga gccccagact cccgtccaga
     3961 gtcctgccca gtcgagcgtg tcttcatcta ccgtaccgtc gccggaccac gtttcgacat
     4021 gccaggattc gtctggaaca tggaccaaga ctccaccatg cgtttctccc gtcaaatcat
     4081 caccaccaac ggactcggag gataagagct ccgcatcggc cgctgtcatc agatcgccat
     4141 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     4201 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     4261 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     4321 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     4381 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     4441 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     4501 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     4561 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     4621 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
     4681 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     4741 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     4801 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     4861 tgtttgaatt cccgcggcgc atcaggcggc ctataacttc gtatagcata cattatacga
     4921 agttattacg tagagctcgg tacctcgtac gtaatacgac tcactagtca ttttatatct
     4981 gagtagtacc tttgctttaa atgtccataa aactaatttt ataatcaata aaacaacgtt
     5041 tgtaaatcaa ctgagtttac aagtagagac attgagggat actttcacta tgctaaagtg
     5101 ataatcgacc aaataataac ttcactttgg tatttattcc tgtcttataa atgttatgta
     5161 tgaattaaat tcatatgcat atggctcact ctgacaattt ttaataatct tccagatcaa
     5221 tattgactac cgatgcgggt ggtcttttgc tttgaattct gctgaacttt acaccccgaa
     5281 cagcaatgtg tgcttcagcc taaaaaaaag taagtgtgtt aatcagtgcc cccgattctt
     5341 cattttttgc ccctctctcc cgtttcgtcg gcaaaagaag agaaaataaa gataagtctc
     5401 aagataggtt ggtaatcgct aaagtggttg tgtggataag agtagcaaaa tggcaggaag
     5461 agcactttgc gcgcacacac tgtactcatt gttctggata aaattctctc gttgtttgcc
     5521 gtcggatgtc tgcctctctg cattgagccg gcttcttcac tatctttagt taacctaaaa
     5581 tgccgtttct tttctcgtat ccccactatc ccgttgaggt tctctgctct cttcgctccc
     5641 taccgccagc gagcaactat ccgtgggggc gccttgctcg gaagatgggg gggaagaaag
     5701 aagatttttg ctatttgcac ttgagaaaga gacttttcct gcgtcgatgg tcagagaaca
     5761 gtgtgcagac acttttcagc tacctagaat tacaattgga tatccccgcc tcccaatcca
     5821 cccacccagg gaaaaagaag ggctcgccga aaatcaaagt tatctccagg ctcgcgcatc
     5881 ccaccgagcg gttgacttct ctccaccact tttcatttta accctcgatc gtcagacaca
     5941 gaaatgacaa gtttgtacaa aaaagcaggc ttaatgagta aaggagaaga acttttcact
     6001 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     6061 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     6121 actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat
     6181 tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg
     6241 agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     6301 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa
     6361 cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa
     6421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     6481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     6541 aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct
     6601 gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     6661 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     6721 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     6781 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     6841 tagcattcgt agaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc
     6901 aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga
     6961 gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc
     7021 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
     7081 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
     7141 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     7201 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     7261 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     7321 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     7381 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     7441 caatcaagtg aaagtttatg aaattaattt tcccgctttt gctttttggg gtttccccta
     7501 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     7561 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     7621 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     7681 ttcccaatat accaaacata actgtttaga tcttcgaatg catcgcgcgc accgtacgtc
     7741 tcgaggaatt cctgcaggat atctggatcc acgaagcttc ccatggtgac gtcaccggtt
     7801 ctagatacct aggtgagctc tggtaccctc tagtcaaggc cttaagtgag tcgtattacg
     7861 gactggccgt cgttttacag gccggatggg ccatggcacc ttaacatttc taaattttag
     7921 tttaataaaa aaattttaaa ataatacgag ttttgagact aactttcaat aaaaaccaaa
     7981 aaattgcttc aaaaaagagc tcgcgccgat ttacgataaa cacagcgcgc tacgacactc
     8041 cgaaaaccgc gccgcaccat tacattctcc gccccaccgc acggtttgcg gaaggcgatg
     8101 aaattgctgg gcctaaaatt ttaacccttt ttttcccgtt tttcaactgt tttcccgatt
     8161 ttttcgatat ttttttcagg tttttagtta gaaaagtata taaaaattgc attatttgta
     8221 gttttcgctc gtaatttgtg atagaaaacg gccaatttgc ctagatttgt gattctacca
     8281 agtggaattt ttgggaaaat atcaatattc cacgattttt attcaaatca taacgtttcg
     8341 aaattatcag cttaaataaa actaaaataa ataataaaat actaaaaacc ctgaatttct
     8401 cggttctcgg ttcagcttga gttcgttcta tacacgttat cgatttttgt catttcgttt
     8461 aatcatgggt tttgattcat tttaaatatg aaatttgatt tattcctcgt ttcgaagttg
     8521 gtttgtcttg tccggccggt aaacaaatgt tattaaaaaa aataataaat ttcgaaattt
     8581 ttcgtaatgt tccgcaatcg tgtttttccg tttatctcgt cttcccttcc ttcaaatata
     8641 ctatttattt tctcaattca catattttca gccagccctg ctcaacaagt ggaataaaaa
     8701 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     8761 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     8821 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     8881 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     8941 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     9001 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     9061 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     9121 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     9181 agcatcaggg gctcgcacca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     9241 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9301 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9361 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9421 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9481 acgagttctt ctgagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     9541 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
     9601 cttttaagcc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     9661 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     9721 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat
     9781 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca
     9841 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa
     9901 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct
     9961 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct
    10021 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
    10081 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg
    10141 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttctcgagg
    10201 tcgacaggcc tgcatgcgtc gacataactt cgtatagcat acattatacg aagttatagt
    10261 tgcaggacca ctgcgatttg gagtaccgcg accgtatggt tgctagtaga tcatctaacc
    10321 tggctactgt ggttcaaagc acaacagatt atgtgtacta tgagtacatg ggaaatatta
    10381 ctgttggaac tcctgatcaa aactttattg ttgtattaga taccggatca gccaatctct
    10441 taattccagg aactaattgc acgttagtat tcctgttttg aaattgaata acgtttgtga
    10501 tcacaatttt tcgaaagttt tttacagaac gtactgtgag aaaaagagat tatttaatga
    10561 aaaagcttca tccacatata ttgcaaccaa ccgcccatgg cagatcaaat atgctagcgg
    10621 tgatgcctac gggacattgg gaattgacac tgttaaggtt atttgatgga aattctgaaa
    10681 ttttacatat ttaaatgcat aaataaattg taaaaaagta ttaacaaaaa tttaattagg
    10741 agtaaattcg tgtctggtac tgtgaataac ttttcttgga gtcatctagg ataaaatgga
    10801 attttcagat cggtggatca ggagaagcac aactcgctat tccaagatca taccttggtg
    10861 ttgcagacac tgtcggatca gatttcaaat gggttcgttt ttttcaagtt ccttcacttt
    10921 cactattttg catttgcaga gcccaaaaga gggaatcttt ggtcttgcat ttactgcatt
    10981 agctgttgac aacattaccc ctccaatcat caacgctgta cccagctttt gttcccttta
    11041 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    11101 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    11161 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11221 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11281 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11881 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    11941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13201 cacatttccc cgaaaagtgc cac
//
