LOCUS       sequence-356941-        3641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a 
            multiple cloning site(MCS) followed by a poly-Adenylation Signal 
            (pA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP210-AAV-0.4CaMKII P-MCS-pA
FEATURES             Location/Qualifiers
     source          1..3641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    708..829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(745..764)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     799..818
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   904..1044
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   904..1033
                     /label=AAV2 ITR
     rep_origin      1119..1574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1206..1225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1416..1437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1591..1610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1710..1732
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1770..1788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1856..1960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1961..2029,2030..2821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2179..2198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2992..3580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3481..3500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcaattc agtcgataac tataacggtc ctaaggtagc gatttaaata
      181 cgcgctctct taaggtagcc ccgggacgcg tacttgtgga ctaagtttgt tcacatcccc
      241 ttctccaacc ccctcagtac atcaccctgg gagaacaagg tccacttgct tctgggccca
      301 cacagtcctg cagtattgtg tatataaggc cagggcaacg gaggagcagg ttttgaagtg
      361 aaaggcaggc aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag
      421 gtggttgcca tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt
      481 gccctgctca gaagccccaa gctcgtcaat caagctggtt ctccatttgc actcaggagc
      541 acgggcaggc gagtggcccc tagttctggg agcagagatc tggatccggt accgaattcg
      601 cggccgcctc gagaagcttc acatttgtag aggttttact tgctttaaaa aacctcccac
      661 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      721 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      781 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaaga
      841 tatcctctct taaggtagca tcgagattta aattagggat aacagggtaa tggcgcgggc
      901 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
      961 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     1021 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     1081 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     1141 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     1201 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1261 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1321 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1381 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1441 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     1501 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1561 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     1621 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     1681 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     1741 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1801 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2101 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2161 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2221 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2281 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2341 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2401 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2461 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2521 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2581 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2641 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2701 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2761 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2821 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2881 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2941 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3121 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3181 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3241 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3301 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3361 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3421 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3481 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3541 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3601 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
