LOCUS       sequence-356945-        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""label stable F-actin; control for neutrophil-specific knockout"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3520..3544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3546..3562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3572..3591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3572..3590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3620..3629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4451..4460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4457..4459,4460..4462,4463..5176)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4457..4459,4460..4462,4463..5173)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4763..4782)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5110..5131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5207..5225)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(5254..5278)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5291..5307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5323..5457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6727..6747)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6755..6771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6787..6921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7360..7547,7548..7559)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7593..7609)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7593..7609)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7646..7664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7685..7701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7698..7720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7709..7725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7733..7739,7740..7757,7758..7763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7778..7799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7916..7933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8087..8675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8167..8186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8846..9637,9638..9706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9469..9488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9707..9811)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt
     2161 atgtgaacat ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa
     2221 accacttgtg actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa
     2281 gtactcttga atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta
     2341 taatttgtaa caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc
     2401 tgtaaatatt taggttattt atttattaat acaatataga aagtaacaaa acacaatgta
     2461 aaaaatcaat aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat
     2521 tagcaacaaa gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct
     2581 gtattaaata atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt
     2641 actggttgtt aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat
     2701 aaaggccatg tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga
     2761 gattttacaa tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa
     2821 ttacccaact ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata
     2881 ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta
     2941 ccagtatcta tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat
     3001 ataatggatt gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt
     3061 aacaaaaaat aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg
     3121 ctttatgtac actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt
     3181 ttctgaattc tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca
     3241 tcatgagctc gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa
     3301 tcactttctc ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt
     3361 cagttattgc ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa
     3421 cacaaacctt tctctggact tataaataaa ggcattcagc agctccacca tacacctcca
     3481 ctggtcttga taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca
     3541 ggcttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     3601 taccgggccc cccctcgagg ccaccatggc caagtatgga gaacatgaag ccagtcctga
     3661 caatgggcag aacgaattca gtgatatcat taagtccaga tctgatgaac acaatgacgt
     3721 acagaagaaa acctttacca aatggataaa tgctcgattt tcaaagagtg ggaaaccacc
     3781 catcaatgat atgttcacag acctcaaaga tggaaggaag ctattggatc ttctagaagg
     3841 cctcacagga acatcactgc caaaggaacg tggttccaca agggtacatg ccttaaataa
     3901 cgtcaacaga gtgctgcagg ttttacatca gaacaatgtg gaattagtga atataggggg
     3961 aaccgacatt gtggatggaa atcacaaact gactttgggg ttactttgga gcatcatttt
     4021 gcactggcag gtgaaagatg tcatgaagga tgtcatgtcg gacctgcagc agacgaacag
     4081 tgagaagatc ctgctcagct gggtgcgtca gaccaccagg ccctacagcc aagtcaacgt
     4141 cctcaacttc accaccagct ggacagatgg actcgccttt aatgctgtcc tccaccgaca
     4201 taaacctgat ctcttcagct gggataaagt tgtcaaaatg tcaccaattg agagacttga
     4261 acatgccttc agcaaggctc aaacttattt gggaattgaa aagctgttag atcctgaaga
     4321 tgttgccgtt cggcttcctg acaagaaatc cataattatg tatttaacat ctttgtttga
     4381 ggtgctacct cagcaagtca ccatagaccg aattctgcag tcgacggtac cgcgggcccg
     4441 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4501 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4561 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4621 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4681 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4741 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4801 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4861 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4921 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4981 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5041 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5101 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5161 cgagctgtac aagtaagcgg ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     5221 taattgcgcg cttggcgtaa tcatggtcat agcacccagc tttcttgtac aaagttgaga
     5281 tctataattc actggccgtc gttttacggt accatcgatg atgatccaga catgataaga
     5341 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     5401 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     5461 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     5521 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcccttgggt
     5581 agcagcgaag tcgaggcatt tctgtcctgg ctggtctaga ggtctctcag gtttaaaaaa
     5641 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt taaacttgct
     5701 atgctgtttc cagcatagct cttaaacaga gacggtcgac agtctgcagt gtcgtctctc
     5761 gaactaggag cctggagaac tgctatataa acccgggact tctgggtatg tttctgccat
     5821 gtggtaagtg gataaaccta cttattcaac tcatttacct caaacatgac agtaaacctc
     5881 ttcggggcaa acctgcaaac gcttaaaacc ttcaaagttt gaaaccagca gttttgtaga
     5941 ggtttaccgt agtatttttg aactgtaaag catatgcaga ggaagctggt tttaggcttg
     6001 tgtgattgga tgagttcttc tgtgacgtaa ctgatctttt gtcctcgtgt ccttcgcctt
     6061 cccccacagg catgcgcgga ccttcccccg ccgcagacca gaggacaaca tgctcagacg
     6121 ccagcaaacg gcccatcgaa acattcacag actctcggat tggagagacc gggcccgagg
     6181 tctctgacta aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
     6241 tatttaaact tgctatgctg tttccagcat agctcttaaa cagagacggt cgacagtctg
     6301 cagtgtcgtc tctcgaacca agagctggag ggagagctat atataccagg gacttctggg
     6361 tatgtttttg ggaggtggtg agtgactaaa ccacttattc agctccctta gatcaagtct
     6421 gaccctatat catggtgaca taaacctgca aactgataaa acctgaagga tctcaaatcc
     6481 agagtttgtg tgagggatta ccgtggtaat tttcaaactg taaagcatat gcaaaattat
     6541 ctggtcttgg cttgagtgat tgggtgtctc ggtgtgatgc agggacgttt tcagtgacgt
     6601 gtccttctcc ctcccccaca ggcatgcgca gaacatttcc cccctccttg aagaccagaa
     6661 caaaagacgc cgagagcagg aaactcgtct tactgaatga ccgaggctgg agaaatcgac
     6721 cctaggcaac tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc
     6781 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6841 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6901 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6961 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     7021 tatgatcctc tagatcagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
     7081 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     7141 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     7201 agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa
     7261 tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc
     7321 aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag
     7381 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
     7441 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
     7501 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg
     7561 ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag cggccgccac
     7621 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     7681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8701 ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg agattatcaa
     8761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
//
