LOCUS       sequence-356946-        4222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with p15A origin for constitutive mNeonGreen expression in 
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4222)
  TITLE     Plasmids with p15A origin for constitutive fluorescent protein 
            expression in E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmr101A-cPr-mNeonGreen (ErmR)
FEATURES             Location/Qualifiers
     source          1..4222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(50..505)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(187..208)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     399..418
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     681..700
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1597..1615)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1611..1658
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3014..3031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3148..3693)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3228..3247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actcctgcga aaagccccaa aaacaggaag attgtataag caaatattta aattgtaaac
       61 gtcaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      121 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt
      181 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      241 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
      301 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      361 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      421 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
      481 cttaatgcgc cgctacaggg cgcgtcctca attaggaagc agcccagtag taggttgagg
      541 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      601 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      661 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      721 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgaagatct aatacttgac
      781 atatcactgt gattcacata taatatgcga aggagccatg gatggtctca aaaggggagg
      841 aggacaacat ggcatccttg ccagcaactc acgaattaca tattttcgga agtattaatg
      901 gtgttgactt tgatatggtt ggacaaggca ctggtaatcc aaacgatgga tacgaggaac
      961 ttaatcttaa atcaacaaaa ggcgatttgc aattttcccc ctggattctt gtgccccata
     1021 ttgggtacgg ctttcatcaa tatttacctt accctgatgg aatgagtcca tttcaagccg
     1081 ctatggtaga cggttctggc taccaagttc atagaaccat gcagtttgaa gacggcgctt
     1141 ccttaacagt aaactacagg tacacgtacg agggttccca tattaaggga gaagcccaag
     1201 ttaagggtac tggttttccg gctgatggtc ccgttatgac taatagcctg acagctgctg
     1261 actggtgtag gtctaaaaag acatatccta acgataaaac aataatttca acttttaagt
     1321 ggtcttatac cactggcaat ggtaaaagat acagatccac tgcccgtact acgtatacat
     1381 tcgctaaacc aatggccgct aattatctta aaaatcaacc catgtacgtg tttaggaaga
     1441 ctgaactaaa acatagtaag accgagttga actttaagga atggcaaaaa gccttcaccg
     1501 atgtcatggg aatggatgaa ttatataagt aatcgagctc aagcttggat ccggctgcta
     1561 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     1621 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     1681 gatatcatcg atacaaattc cccgtaggcg ctagggacct ctttagcttc ttggaagctg
     1741 tcagtagtat atctaataat ttatctccat tccctttagt aacgtgtaac tttccaaatt
     1801 taaaaaagcg actcatagaa ttatttcctc ccgttaaata atagataact attaaaaata
     1861 gacaatactt gctcataagt aatggtactt aaattgttta ctttggcgtg tttcattgct
     1921 tgatgaaact gatttttagt aaacagttga cgatattctc gattgaccca ttttgaaaca
     1981 aagtacgtat atagcttcca atatttatct ggaacatctg tggtatggcg ggtaagtttt
     2041 attaagacac tgtttacttt tggtttagga tgaaagcatt ccgctggcag cttaagcaat
     2101 tgctgaatcg agacttgagt gtgcaagagc aaccctagtg ttcggtgaat atccaaggta
     2161 cgcttgtaga atccttcttc aacaatcaga tagatgtcag acgcatggct ttcaaaaacc
     2221 acttttttaa taatttgtgt gcttaaatgg taaggaatac tcccaacaat tttatacctc
     2281 tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac acgaatgttc
     2341 agttttaatt tttctgacga taagttgaat agatgactgt ctaattcaat agacgttacc
     2401 tgtttactta ttttagccag tttcgtcgtt aaatgccctt tacctgttcc aatttcgtaa
     2461 acggtatcgg tttcttttaa attcaattgt tttattattt ggttgagtac tttttcactc
     2521 gttaaaaagt tttgagaata ttttatattt ttgttcatgt aattactcct gaagtgatta
     2581 catctgtaaa taaatacaga agttaaacga tttgtttgta attttagtta tctgtttaaa
     2641 aagtcataag attagtcact ggtaggaatt aatctaacgt atttatttat ctgcgtaatc
     2701 actgttttta gtctgtttca aaacagtaga tgttttatct acattacgca tttggaatac
     2761 caacatgacg aatccctcct tcttaattac aaatttttag catctaattt aacttcaatt
     2821 cctattatac acaaaatttt aagatactgc actatcaaca cactcttaag tttgcttcta
     2881 aggctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
     2941 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
     3001 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     3061 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     3121 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     3181 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     3241 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     3301 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     3361 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     3421 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     3481 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     3541 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     3601 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     3661 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac
     3721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4141 gttaatagtt tgcgcaacgt tgttgccatt gctgcagggt tgaaggctct caagggcatc
     4201 ggtcgacgct ctcccttatg cg
//
