LOCUS       sequence-356948-        9256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""label active Rac under Rac2 neutrophil-specific knockout 
            background"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-PBD-GFP-U6ac-rac2 guides
FEATURES             Location/Qualifiers
     source          1..9256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3520..3544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3546..3562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3572..3590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3657..3659,3660..3662,3663..4373)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3657..3659,3660..3662,3663..4373)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3963..3982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4310..4331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4659..4677)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4706..4730)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4743..4759)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4775..4909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6153..6173)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6181..6197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6213..6347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6786..6973,6974..6985)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7019..7035)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7019..7035)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7072..7090)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8272..9063,9064..9132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8895..8914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9133..9237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt
     2161 atgtgaacat ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa
     2221 accacttgtg actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa
     2281 gtactcttga atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta
     2341 taatttgtaa caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc
     2401 tgtaaatatt taggttattt atttattaat acaatataga aagtaacaaa acacaatgta
     2461 aaaaatcaat aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat
     2521 tagcaacaaa gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct
     2581 gtattaaata atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt
     2641 actggttgtt aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat
     2701 aaaggccatg tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga
     2761 gattttacaa tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa
     2821 ttacccaact ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata
     2881 ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta
     2941 ccagtatcta tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat
     3001 ataatggatt gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt
     3061 aacaaaaaat aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg
     3121 ctttatgtac actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt
     3181 ttctgaattc tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca
     3241 tcatgagctc gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa
     3301 tcactttctc ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt
     3361 cagttattgc ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa
     3421 cacaaacctt tctctggact tataaataaa ggcattcagc agctccacca tacacctcca
     3481 ctggtcttga taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca
     3541 ggcttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     3601 taccgggccc cccctcgaga atacaagcta cttgttcttt ttgcaggatc caaaccatgg
     3661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3841 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3901 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3961 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4021 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4081 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4141 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4201 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4261 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4321 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggaa
     4381 aagagcgccc agagatctct cttccttcag actttgagca tacgattcat gtgggttttg
     4441 atgcagtcac cggggaattc actggaattc ctgagcaatg ggcacgacta ctccaaacct
     4501 ccaacataac aaagctggaa cagaagaaga acccacaagc tgttcttgat gttctcaagt
     4561 tctacgactc caaagaaacg gtcaacaacc agaaatctag aactatagtg agtcgtatta
     4621 cgtagatcgc ggccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     4681 cgcttggcgt aatcatggtc atagcaccca gctttcttgt acaaagttga gatctataat
     4741 tcactggccg tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga
     4801 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4861 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4921 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     4981 aaacctctac aaatgtggta tggctgatta tgatcctcta gatcccttgg gtagcagcga
     5041 agtcgaggca tttctgtcct ggctggtcta gaggtctctc aggtttaaaa aaagcaccga
     5101 ctcggtgcca ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgtt
     5161 tccagcatag ctcttaaaca cagggtctcg cattggcccg aactaggagc ctggagaact
     5221 gctatataaa cccgggactt ctgggtatgt ttctgccatg tggtaagtgg ataaacctac
     5281 ttattcaact catttacctc aaacatgaca gtaaacctct tcggggcaaa cctgcaaacg
     5341 cttaaaacct tcaaagtttg aaaccagcag ttttgtagag gtttaccgta gtatttttga
     5401 actgtaaagc atatgcagag gaagctggtt ttaggcttgt gtgattggat gagttcttct
     5461 gtgacgtaac tgatcttttg tcctcgtgtc cttcgccttc ccccacaggc atgcgcggac
     5521 cttcccccgc cgcagaccag aggacaacat gctcagacgc cagcaaacgg cccatcgaaa
     5581 cattcacaga ctctcggatt ggagagaccg ggcccgaggt ctctgactaa aaaagcaccg
     5641 actcggtgcc actttttcaa gttgataacg gactagcctt atttaaactt gctatgctgt
     5701 ttccagcata gctcttaaac gggactctgg gatacagccc gaaccaagag ctggagggag
     5761 agctatatat accagggact tctgggtatg tttttgggag gtggtgagtg actaaaccac
     5821 ttattcagct cccttagatc aagtctgacc ctatatcatg gtgacataaa cctgcaaact
     5881 gataaaacct gaaggatctc aaatccagag tttgtgtgag ggattaccgt ggtaattttc
     5941 aaactgtaaa gcatatgcaa aattatctgg tcttggcttg agtgattggg tgtctcggtg
     6001 tgatgcaggg acgttttcag tgacgtgtcc ttctccctcc cccacaggca tgcgcagaac
     6061 atttcccccc tccttgaaga ccagaacaaa agacgccgag agcaggaaac tcgtcttact
     6121 gaatgaccga ggctggagaa atcgacccta ggcaacttta ttatacatag ttgataattc
     6181 actggccgtc gttttacggt accatcgatg atgatccaga catgataaga tacattgatg
     6241 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6301 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6361 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     6421 acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg
     6481 ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga
     6541 atcacatact tcctccattt tcttccactg attgactgtt ataatttccc taatttccag
     6601 gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat
     6661 gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac
     6721 atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata
     6781 gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
     6841 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
     6901 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
     6961 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga
     7021 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     7081 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8161 aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9241 atttccccga aaagtg
//
