LOCUS       sequence-356951-        9612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Rescue Rac-CA expression in neutrophil specific knockout"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-Rac-CA(guide
            resistant)-2a-mcherry-U...
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3519..3543
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3545..3561
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3571..3590
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3571..3589
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3627..3636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3633..4340
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3778..3796)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4046..4065
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4350..4406
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(5016..5034)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(5063..5087)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5100..5116)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5132..5266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6510..6530)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6538..6554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6570..6704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7143..7330,7331..7342)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7376..7392)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7376..7392)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7428..7446)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7467..7483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7480..7502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7491..7507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7515..7521,7522..7539,7540..7545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7560..7581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7698..7715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7869..8457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7949..7968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8628..9419,9420..9488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9251..9270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9489..9593)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt tttcagaaag aaacggtcca aaaagagtta
     2161 tgtgaacatt tatccatgtg tttaatgttt tttaaattaa aaaaaaacat ttttattaaa
     2221 ccacttgtga ctaaaccttt tttagttatt ctattttgtg acgagtctga atggccaaag
     2281 tactcttgaa tccaaaatct ttatagtaaa atgtttaatc ttacttgcag tgttgggtat
     2341 aatttgtaac aaagttgaat tccatatctg ctgagtgaaa aagtaaagta agagattgct
     2401 gtaaatattt aggttattta tttattaata caatatagaa agtaacaaaa cacaatgtaa
     2461 aaaatcaata aagaaagtaa atattaaaag taatgataaa ctaatgatgt aaacaatatt
     2521 agcaacaaag tttgtacaat tcagggaggg taaagaattt cacctaatag tcaaactctg
     2581 tattaaataa tataaaatct ttgttcttag tctgaagagg agactgtgag agaaagctta
     2641 ctggttgtta aattagaaaa tttgtaactt gcagattaaa atgatttaaa taattttata
     2701 aaggccatgt ttgaaacaaa tgtaaaaaca taataaatgt taagaatttc agatattgag
     2761 attttacaat ttaaaaaaag caataaagta atagcagaga cctacactct taaagtaaat
     2821 tacccaactc tgcttactag taacttttgg aatagttact agtatccatt gaacaaatac
     2881 tagaatctaa ttaaattgtg tgagtgtcca atgaaaagat tcttgtattt aaaacagtac
     2941 cagtatctat atatgtacaa aatttagtgc tagcaaaatg caaataaata ctactgcata
     3001 taatggattg cattgtaaat cgactcgcca taatacattt atttcctatt tttactttta
     3061 acaaaaaata agttcactga gtatttcatg tcattaaatc gcattaaacc tcttgcatgc
     3121 tttatgtaca ctagtgcagt tcgctaaatg accaccggtg tcactaataa cacgatcgtt
     3181 tctgaattct gcattaaacg ccatttatac gttgctctta ttagtaaaca atttctccat
     3241 catgagctcg accagtattg catatttcat cctcttaaat ccagagttgt ttcttttaat
     3301 cactttctct tcatgataaa agcagttttt ggttttcaga agtttacaac aaacccagtc
     3361 agttattgcc tcagcaaaca ctggagcaac tgcacacttg tgaaaagctt cttgcgaaac
     3421 acaaaccttt ctctggactt ataaataaag gcattcagca gctccaccat acacctccac
     3481 tggtcttgat aaagggatcc tttaagggcg aattcgaccc aagtttgtac aaaaaagcag
     3541 gcttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     3601 accgggcccc ccctcgaggt cgactcgcca ccatggtgag caagggcgag gaggataaca
     3661 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     3721 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     3781 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     3841 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     3901 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     3961 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     4021 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4081 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     4141 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     4201 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     4261 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     4321 gcatggacga gctgtacaag ggatctggag ccacgaactt ctctctgtta aagcaagcag
     4381 gagacgtgga agaaaacccc ggtcctatgc aagcaataaa gtgtgtggtg gtcggagatg
     4441 gagctgtggg aaagacctgt cttctcatca gctacactac caatgcgttc cccggggagt
     4501 acattcccac agtgtttgat aactactctg caaatgtaat ggtggatagc aaaccggtaa
     4561 atctaggcct atgggacacc gcaggcctgg aagattatga cagactgcgg ccactctcct
     4621 acccgcagac ggatgtgttt cttatctgtt tctctttggt gagcccagca tcattcgaaa
     4681 atgtcagagc caagtggtac ccagaggtga ggcatcactg cccttccact ccaattatcc
     4741 tggttggcac caagcttgac ttgagagatg agaaggagac catcgagaag ctgaaggaga
     4801 agaaactggc accgatcact tatccgcaag gacttgcctt agcaaaggaa atagatgcag
     4861 taaaatacct ggagtgttcg gccctcactc agagagggct aaaaacagtg tttgatgagg
     4921 cgattcgcgc tgtgctctgc ccacagccca ccaaggtcaa gaagaagggc tgcgtgatgc
     4981 tctaagcggc cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     5041 ttggcgtaat catggtcata gcacccagct ttcttgtaca aagttgagat ctataattca
     5101 ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat acattgatga
     5161 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5221 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5281 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5341 cctctacaaa tgtggtatgg ctgattatga tcctctagat cccttgggta gcagcgaagt
     5401 cgaggcattt ctgtcctggc tggtctagag gtctctcagg tttaaaaaaa gcaccgactc
     5461 ggtgccactt tttcaagttg ataacggact agccttattt aaacttgcta tgctgtttcc
     5521 agcatagctc ttaaacacag ggtctcgcat tggcccgaac taggagcctg gagaactgct
     5581 atataaaccc gggacttctg ggtatgtttc tgccatgtgg taagtggata aacctactta
     5641 ttcaactcat ttacctcaaa catgacagta aacctcttcg gggcaaacct gcaaacgctt
     5701 aaaaccttca aagtttgaaa ccagcagttt tgtagaggtt taccgtagta tttttgaact
     5761 gtaaagcata tgcagaggaa gctggtttta ggcttgtgtg attggatgag ttcttctgtg
     5821 acgtaactga tcttttgtcc tcgtgtcctt cgccttcccc cacaggcatg cgcggacctt
     5881 cccccgccgc agaccagagg acaacatgct cagacgccag caaacggccc atcgaaacat
     5941 tcacagactc tcggattgga gagaccgggc ccgaggtctc tgactaaaaa agcaccgact
     6001 cggtgccact ttttcaagtt gataacggac tagccttatt taaacttgct atgctgtttc
     6061 cagcatagct cttaaacggg actctgggat acagcccgaa ccaagagctg gagggagagc
     6121 tatatatacc agggacttct gggtatgttt ttgggaggtg gtgagtgact aaaccactta
     6181 ttcagctccc ttagatcaag tctgacccta tatcatggtg acataaacct gcaaactgat
     6241 aaaacctgaa ggatctcaaa tccagagttt gtgtgaggga ttaccgtggt aattttcaaa
     6301 ctgtaaagca tatgcaaaat tatctggtct tggcttgagt gattgggtgt ctcggtgtga
     6361 tgcagggacg ttttcagtga cgtgtccttc tccctccccc acaggcatgc gcagaacatt
     6421 tcccccctcc ttgaagacca gaacaaaaga cgccgagagc aggaaactcg tcttactgaa
     6481 tgaccgaggc tggagaaatc gaccctaggc aactttatta tacatagttg ataattcact
     6541 ggccgtcgtt ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt
     6601 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     6661 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     6721 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     6781 tctacaaatg tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca
     6841 cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc
     6901 acatacttcc tccattttct tccactgatt gactgttata atttccctaa tttccaggtc
     6961 aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa
     7021 catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt
     7081 taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa
     7141 tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa
     7201 agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg
     7261 tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt
     7321 actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc
     7381 actagttcta gagcggccgc caccgcggtg agctccagct tttgttccct ttagtgaggg
     7441 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7501 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7561 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7621 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8281 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggc
     8521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8581 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8641 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8701 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8761 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8821 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8881 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8941 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9001 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9061 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9121 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9181 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9241 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9301 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9361 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9421 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9481 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9541 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9601 ccccgaaaag tg
//
