LOCUS       sequence-356954-       10868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-LacI-HIPK2 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Haas J, Bloesel D, Bacher S, Kracht M, Schmitz ML
  TITLE     Chromatin Targeting of HIPK2 Leads to Acetylation-Dependent 
            Chromatin Decondensation.
  JOURNAL   Front Cell Dev Biol. 2020 Sep 1;8:852. doi: 
            10.3389/fcell.2020.00852. eCollection 2020.
  PUBMED    32984337
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2020-09-1"; volume: "8:852. doi"; pages: " 
            10.3389/fcell.2020.00852. eCollection 2020"
COMMENT     Sequence Label: GFP-LacI-HIPK2
FEATURES             Location/Qualifiers
     source          1..10868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        155..534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        535..738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     689..709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     783..802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        783..801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      851..860
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(857..859,860..862,863..1573)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(857..859,860..862,863..1573)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(902..923)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1163..1182)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1510..1531
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1611..1630)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2690..2710
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6379..6396)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6385..6609
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6655..7083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6742..6761)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6952..6973
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7092..7112)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7097..7426
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7277..7412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7339..7358
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7493..8287
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7547..7566)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8157..8176
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8461..8582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8498..8517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8552..8571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8631..8647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8631..8647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8644..8666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8655..8671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8679..8685,8686..8703,8704..8709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8724..8745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8862..8879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9033..9621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9113..9132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9792..10583,10584..10652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10415..10434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10653..10757)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10832..10851)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
       61 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      121 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      241 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      301 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      361 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      421 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      481 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      541 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      601 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      661 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      721 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
      781 attaatacga ctcactatag ggagacccaa gctggctagc gctaccgggg ccgctctagc
      841 gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      901 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      961 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1021 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1081 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1141 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1201 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1261 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1321 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1381 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1441 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1501 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1561 cgagctgtac aagtccggac tcagatccat ggtgaaacca gtaacgttat acgatgtcgc
     1621 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     1681 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     1741 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     1801 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     1861 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     1921 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
     1981 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
     2041 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
     2101 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
     2161 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
     2221 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
     2281 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
     2341 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
     2401 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     2461 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     2521 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     2581 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2641 acgacaggtt tcccgaagca gcctgagggg gcgtcgactg ggtacccctc ctaagaagaa
     2701 gaggaaggtt gcccgggatc taggatccac tgtgtctagc gcctcacatg tgcaagtttt
     2761 ctcccctcac acccttcaat caagtgcctt ctgtagtgtg aagaaactaa aagtagagcc
     2821 aagttccaac tgggacatga ctgggtacgg ctcccacagc aaagtgtaca gccagagcaa
     2881 gaacatacca ccttctcagc cagcctccac aaccgtcagc acctccttgc cggtcccaaa
     2941 cccaagccta ccttacgagc agaccatcgt cttcccagga agcaccgggc acatcgtggt
     3001 cacctcagca agcagcactt ctgtcaccgg gcaagtcctc ggcggaccac acaacctaat
     3061 gcgtcgaagc actgtgagcc tccttgatac ctaccaaaaa tgtggactca agcgtaagag
     3121 cgaggagatc gagaacacaa gcagcgtgca gatcatcgag gagcatccac ccatgattca
     3181 gaataatgca agcggggcca ctgtcgccac tgccaccacg tctactgcca cctccaaaaa
     3241 cagcggctcc aacagcgagg gcgactatca gctggtgcag catgaggtgc tgtgctccat
     3301 gaccaacacc tacgaggtct tagagttctt gggccgaggg acgtttggac aagtggtcaa
     3361 gtgctggaaa cggggcacca atgagatcgt agccatcaag atcctgaaga accgcccatc
     3421 ctatgcccga caaggtcaga ttgaagtgag catcctggcc cggttgagca cggagagtgc
     3481 cgatgactat aacttcgtcc gggcctacga atgcttccag cacaagaacc acacgtgctt
     3541 ggtcttcgag atgttggagc agaacctcta tgactttctg aagcaaaaca agtttagccc
     3601 cttgcccctc aaatacattc gcccagttct ccagcaggta gccacagccc tgatgaaact
     3661 caaaagccta ggtcttatcc acgctgacct caaaccagaa aacatcatgc tggtggatcc
     3721 atctagacaa ccatacagag tcaaggtcat cgactttggt tcagccagcc acgtctccaa
     3781 ggctgtgtgc tccacctact tgcagtccag atattacagg gcccctgaga tcatccttgg
     3841 tttaccattt tgtgaggcaa ttgacatgtg gtccctgggc tgtgttattg cagaattgtt
     3901 cctgggttgg ccgttatatc caggagcttc ggagtatgat cagattcggt atatttcaca
     3961 aacacagggt ttgcctgctg aatatttatt aagcgccggg acaaagacaa ctaggttttt
     4021 caaccgtgac acggactcac catatccttt gtggagactg aagacaccag atgaccatga
     4081 agcagagaca gggattaagt caaaagaagc aagaaagtac attttcaact gtttagatga
     4141 tatggcccag gtgaacatga cgacagattt ggaagggagc gacatgttgg tagaaaaggc
     4201 tgaccggcgg gagttcattg acctgttgaa gaagatgctg accattgatg ctgacaagag
     4261 aatcactcca atcgaaaccc tgaaccatcc ctttgtcacc atgacacact tactcgattt
     4321 tccccacagc acacacgtca aatcatgttt ccagaacatg gagatctgca agcgtcgggt
     4381 gaatatgtat gacacggtga accagagcaa aacccctttc atcacgcacg tggcccccag
     4441 cacgtccacc aacctgacca tgacctttaa caaccagctg accactgtcc acaaccaggc
     4501 tccctcctct accagtgcca ctatttcctt agccaatccc gaagtctcca tactaaacta
     4561 cccatctaca ctctaccagc cctcagcggc atccatggct gcagtggccc agcggagcat
     4621 gcccctgcag acaggaacag cccagatttg tgcccggcct gacccgttcc agcaagctct
     4681 catcgtgtgt ccccccggct tccaaggctt gcaggcctct ccctctaagc acgctggcta
     4741 ctcggtgcga atggaaaatg cagttcccat cgtcactcaa gccccaggag ctcagcctct
     4801 tcagatccaa ccaggtctgc ttgcccagca ggcttggcca agtgggaccc agcagatcct
     4861 gcttccccca gcatggcagc aactgactgg agtggccacc cacacatcag tgcagcatgc
     4921 caccgtgatt cccgagacca tggcaggcac ccagcagctg gcggactgga gaaatacgca
     4981 tgctcacgga agccattata atcccatcat gcagcagcct gcactattga ccggtcatgt
     5041 gacccttcca gcagcacagc ccttaaatgt gggtgtggcc cacgtgatgc ggcagcagcc
     5101 aaccagcacc acctcctccc ggaagagtaa gcagcaccag tcatctgtga gaaatgtctc
     5161 cacctgtgag gtgtcctcct ctcaggccat cagctcccca cagcgatcca agcgtgtcaa
     5221 ggagaacaca cctccccgct gtgccatggt gcacagtagc ccggcctgca gcacctcggt
     5281 cacctgtggg tggggcgacg tggcctccag caccacccgg gaacggcagc ggcagacaat
     5341 tgtcattccc gacactccca gccccacggt cagcgtcatc accatcagca gtgacacgga
     5401 cgaggaggag gaacagaaac acgcccccac cagcactgtc tccaagcaaa gaaaaaacgt
     5461 catcagctgt gtcacagtcc acgactcccc ctactccgac tcctccagca acaccagccc
     5521 ctactccgtg cagcagcgtg ctgggcacaa caatgccaat gcctttgaca ccaaggggag
     5581 cctggagaat cactgcacgg ggaacccccg aaccatcatc gtgccacccc tgaaaaccca
     5641 ggccagcgaa gtattggtgg agtgtgatag cctggtgcca gtcaacacca gtcaccactc
     5701 gtcctcctac aagtccaagt cctccagcaa cgtgacctcc accagcggtc actcttcagg
     5761 gagctcatct ggagccatca cctaccggca gcagcggccg ggcccccact tccagcagca
     5821 gcagccactc aatctcagcc aggctcagca gcacatcacc acggaccgca ctgggagcca
     5881 ccgaaggcag caggcctaca tcactcccac catggcccag gctccgtact ccttcccgca
     5941 caacagcccc agccacggca ctgtgcaccc gcatctggct gcagccgctg ccgctgccca
     6001 cctccccacc cagccccacc tctacaccta cactgcgccg gcggccctgg gctccaccgg
     6061 caccgtggcc cacctggtgg cctcgcaagg ctctgcgcgc cacaccgtgc agcacactgc
     6121 ctacccagcc agcatcgtcc accaggtccc cgtgagcatg ggcccccggg tcctgccctc
     6181 gcccaccatc cacccgagtc agtatccagc ccaatttgcc caccagacct acatcagcgc
     6241 ctcgccagcc tccaccgtct acactggata cccactgagc cccgccaagg tcaaccagta
     6301 cccttacata taagtcgacg aattctcgag gatcgattaa gtagctaact agagggcccg
     6361 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6421 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6481 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6541 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6601 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     6661 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6721 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6781 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6841 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6901 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6961 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7021 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7081 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7141 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7201 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7261 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7321 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7381 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7441 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     7501 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     7561 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     7621 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     7681 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     7741 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     7801 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     7861 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     7921 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     7981 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     8041 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     8101 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     8161 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     8221 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     8281 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     8341 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8401 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8461 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8521 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8581 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8641 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8701 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8761 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8821 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8881 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8941 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9001 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9061 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9121 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9181 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9241 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9301 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9361 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9421 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9481 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9541 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9601 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9661 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9721 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9781 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9841 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9901 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9961 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10021 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10081 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10141 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10201 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10261 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10321 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10381 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10441 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10501 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10561 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10621 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10681 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10741 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    10801 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    10861 gccagtat
//
