LOCUS       sequence-356958-       11811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express GFP-GluN2A from a TRE3G 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..11811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1950..2328
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1958..1976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1994..2012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2030..2048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2066..2084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2102..2120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2138..2156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2174..2192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2237..2261
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2363..2387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2391..2400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2478..2480,2481..3191)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2478..2480,2481..3191)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2520..2541)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2781..2800)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3128..3149
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7522..7546)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7599..7640
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7664..8252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7717..7737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8135..8146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8332..8565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8637..8758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8674..8693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8728..8747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8798..8933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8953..8972)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8954..8972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8982..8999)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8982..8998)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8991..9013)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9140..9595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9227..9246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9437..9458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9621..9725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9726..9794,9795..10586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9944..9963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10757..11345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11246..11265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11499..11516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11633..11654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11669..11674,11675..11692,11693..11699)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11707..11723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11712..11734
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11731..11747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11731..11747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11766..11786
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11768..11786
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatatc acgagactag cctcgagttg gctttactcc ctatcagtga tagagaacgt
     1981 atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt
     2041 gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta
     2101 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
     2161 atatccagtt tactccctat cagtgataga gaacgtataa gctttaggcg tgtacggtgg
     2221 gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa
     2281 cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaaac tagtccagtg
     2341 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctgcc gccaccatgg
     2401 gcagattggg ctactggacc ttgctggtat tgccggccct tctggtctgg cgcgatccgg
     2461 cgcagaacgc ggctagggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2521 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2581 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2641 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     2701 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2761 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2821 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2881 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2941 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     3001 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3061 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     3121 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     3181 agctgtacaa ggctagcgcg gcggagaagg gtcctccagc gctgaacatt gcggtgctgc
     3241 tgggtcacag ccacgacgtg acagaacgcg aacttcgaaa tctgtggggc ccagagcagg
     3301 caaccggctt gcccctggat gtgaacgtgg tggcgttatt gatgaaccgc actgacccta
     3361 agagcctcat cacgcatgtg tgcgacctca tgtccggggc gcgcatccac ggcttggtgt
     3421 ttggagatga cacggaccag gaggctgtgg cccagatgct ggattttatc tcctcacaga
     3481 cttttatccc catcttgggc attcatgggg gtgcatctat gatcatggct gacaaggatc
     3541 cgacatccac gttcttccag tttggagcct ccatccagca gcaagccaca gttatgctga
     3601 agatcatgca ggactacgac tggcacgtct tctccctggt caccaccatc ttccctggct
     3661 accgagactt catcagcttt atcaagacaa cagtggacaa cagctttgtg ggctgggata
     3721 tgcagaacgt gatcacactg gacacctcct tcgaggacgc caagacgcag gtccagctga
     3781 agaagatcca ttcttctgtc atcctgctct actgctccaa agacgaggct gtcctcatcc
     3841 tgagcgaggc tcgctccttc ggcctcactg gctatgattt cttctggatt gtccccagtt
     3901 tggtgtctgg gaacacagag ctcatcccca aagagtttcc atcaggtctc atttcagtct
     3961 cttatgacga ctgggactac agcctggagg caagagtgag agacggtctt gggatcttaa
     4021 ccactgccgc atcctccatg ttggagaagt tctcctacat tcctgaggcc aaggccagct
     4081 gctatgggca ggcagagaag ccagagaccc cgctacacac cctgcaccaa ttcatggtca
     4141 atgtgacttg ggatggcaag gacttgtcct tcactgagga aggttaccag gtgcacccca
     4201 ggcttgtggt gatcgtgctg aacaaggacc gggagtggga aaaggtgggc aagtgggaga
     4261 accagacgct gagcctgagg cacgctgtgt ggccaaggta caagtccttt tctgactgcg
     4321 agccagatga caaccacctc agcattgtca ccttggagga agcccccttc gtcatcgtag
     4381 aggacataga ccccctgact gagacctgtg tgaggaacac ggtgccctgt cggaagttcg
     4441 tcaagatcaa caattcaacc aacgaaggga tgaatgtgaa gaaatgctgc aaggggttct
     4501 gcatcgacat cctcaagaag ctgtccagaa ctgtgaagtt cacctatgac ctctacctgg
     4561 tgaccaatgg gaagcatggg aaaaaggtta acaatgtgtg gaatggaatg ataggtgaag
     4621 tggtctatca acgagcagtc atggctgtgg gctcactcac catcaatgag gagcgttcgg
     4681 aagtggtgga cttctcggtg ccctttgtgg agacaggaat cagcgtcatg gtctccagga
     4741 gtaatggcac tgtctcccct tctgctttcc tcgaaccctt cagtgcctcc gtctgggtga
     4801 tgatgtttgt gatgctgctc atcgtctcag ccattgctgt cttcgttttt gaatacttca
     4861 gtcctgttgg atacaacaga aacttagcca aagggaaagc tccccacggg ccttctttta
     4921 ctattggaaa agctatatgg ctcctctggg gcctggtctt caacaattct gtgcctgtcc
     4981 agaatcctaa aggcacaacc agcaagatca tggtgtcagt gtgggccttc tttgctgtca
     5041 tcttcctggc cagttacaca gccaacctgg ctgccttcat gatccaggag gagtttgtgg
     5101 accaagtgac tggcctcagt gacaagaagt tccagagacc tcatgactat tctccacctt
     5161 tccgatttgg gacggtaccc aatggaagta cagagaggaa tattcgtaac aactacccgt
     5221 atatgcacca gtacatgacc agattcaacc agaggggagt ggaggatgcc ttggtcagct
     5281 tgaaaaccgg gaagttggac gctttcatct atgacgcagc cgtcttgaac tacaaggccg
     5341 ggagggatga aggctgtaaa ctggtgacca ttgggagcgg gtacatcttt gctaccacag
     5401 gctatggaat tgcgctgcag aagggctcac cctggaagag gcagattgac ctcgctctgc
     5461 tccagtttgt tggtgatggt gagatggagg agctggagac actgtggctt acgggcatct
     5521 gccacaacga gaagaatgag gtgatgagta gccagctgga catcgataac atggcgggcg
     5581 tgttctacat gctggctgca gccatggccc tcagcctcat caccttcatc tgggagcacc
     5641 tcttctactg gaagctgcgc ttctgcttca caggcgtgtg ctctgaccgg cccgggctgc
     5701 tcttctccat cagcaggggc atctatagtt gcatccatgg ggtacacatt gaagaaaaga
     5761 agaaatctcc agatttcaat ctgactggat cacagagcaa catgctaaag cttcttcggt
     5821 cagctaaaaa catctccaat atgtccaaca tgaactcctc aagaatggac tcacctaaaa
     5881 gagctactga cttcattcaa agagggtcac ttattgtgga catggtttca gacaagggaa
     5941 atttgatata ttcagacaac agatcctttc aagggaagga cagtatattt ggagacaaca
     6001 tgaatgaact ccaaacattt gtggccaaca ggcacaagga taatctcagt aactatgtgt
     6061 ttcaaggaca gcatcctctc actctcaatg agtccaaccc taacacagta gaggtggctg
     6121 tcagcactga atccaaaggg aactcccgac cccggcagct ttggaagaaa tccatggagt
     6181 ctctacgcca ggattctcta aaccagaacc cagtctccca gagggatgag aagactgcag
     6241 agaatcggac ccactcgcta aagagtccta ggtatcttcc agaagaggta gcccactctg
     6301 acatttcaga aacctcaagc cgggccacat gccacaggga gccagataac aataagaacc
     6361 acaagaccaa ggataacttc aaacggtcaa tggcctctaa gtatcccaag gactgtagcg
     6421 atgttgaccg cacctacatg aaaaccaaag caagttctcc cagggataag atctatacca
     6481 ttgatggtga gaaggagccc agcttccact tagatcctcc tcagtttgtt gagaatataa
     6541 ccctgcctga gaatgtgggc ttcccagata cctaccaaga tcacaatgag aacttccgca
     6601 agggggactc cacactgccc atgaacagga acccattaca taatgaagac gggcttccca
     6661 acaatgacca atataaactc tatgccaagc actttacctt gaaagacaag ggttccccac
     6721 acagtgaggg cagtgatcga taccggcaga actccacaca ttgcagaagc tgcctttcga
     6781 atctgcccac ctactcaggc cactttacca tgaggtctcc tttcaagtgt gatgcctgtc
     6841 tgcggatggg gaatctctat gacattgatg aagaccagat gcttcaggag acaggtaacc
     6901 cagctactcg ggaggaggtc taccagcagg actggtcaca gaacaacgcc ctccagttcc
     6961 agaagaacaa gctaaggatt aaccgacagc actcctatga taacattctg gacaaaccca
     7021 gagagataga ccttagcagg ccctcccgga gcataagcct caaggacagg gaacggctac
     7081 tggagggcaa cttgtatggg agcctgttca gtgtcccctc aagcaaactc ttggggaaca
     7141 aaagctccct tttcccccaa ggtctggagg acagcaagag gagcaagtct ctcttgccag
     7201 accacgcctc cgataatcct ttcctccaca cgtatgggga tgaccaacgc ttagttatcg
     7261 ggagatgtcc ctcggaccct tacaaacact cattgccatc acaggcggta aatgacagct
     7321 atcttcggtc atccttgagg tcaacagcat catattgctc cagggacagt cggggccaca
     7381 gtgatgtgta tatttcagag catgttatgc cttatgctgc aaataagaat accatgtact
     7441 ctacccccag ggttttaaat tcctgcagca atagacgagt gtacaagaaa atgcctagta
     7501 tcgaatctga tgtttaagat cacccagctt tcttgtacaa agtggttgat atccagcaca
     7561 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     7621 tcctcggtct cgattctacg cgtaccggtt agtaatgatc gacaatcaac ctctggatta
     7681 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7741 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7801 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7861 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7921 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7981 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8041 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     8101 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8161 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8221 gagtcggatc tccctttggg ccgcctcccc gcctggcgat ggtaccttta agaccaatga
     8281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8341 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8581 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8641 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8701 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8761 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8821 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8881 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8941 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9001 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9061 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9121 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9541 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11521 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11581 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11641 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11701 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11761 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
