LOCUS       sequence-356960-       10574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""label active Rac; control for neutrophil-specific knockout"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-RacFRET-U6ac-ctrl guides
FEATURES             Location/Qualifiers
     source          1..10574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3520..3544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3546..3562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3572..3591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3572..3590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3629..3631,3632..3634,3635..4147)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             join(5135..5137,5138..5140,5141..5653)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(5951..5969)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(5998..6022)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6035..6051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6067..6201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7471..7491)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7499..7515)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7531..7665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8104..8291,8292..8303)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8337..8353)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8337..8353)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8390..8408)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8429..8445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8442..8464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8453..8469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8477..8483,8484..8501,8502..8507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8522..8543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8660..8677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8831..9419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8911..8930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9590..10381,10382..10450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10213..10232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10451..10555)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt
     2161 atgtgaacat ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa
     2221 accacttgtg actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa
     2281 gtactcttga atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta
     2341 taatttgtaa caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc
     2401 tgtaaatatt taggttattt atttattaat acaatataga aagtaacaaa acacaatgta
     2461 aaaaatcaat aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat
     2521 tagcaacaaa gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct
     2581 gtattaaata atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt
     2641 actggttgtt aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat
     2701 aaaggccatg tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga
     2761 gattttacaa tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa
     2821 ttacccaact ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata
     2881 ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta
     2941 ccagtatcta tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat
     3001 ataatggatt gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt
     3061 aacaaaaaat aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg
     3121 ctttatgtac actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt
     3181 ttctgaattc tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca
     3241 tcatgagctc gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa
     3301 tcactttctc ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt
     3361 cagttattgc ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa
     3421 cacaaacctt tctctggact tataaataaa ggcattcagc agctccacca tacacctcca
     3481 ctggtcttga taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca
     3541 ggcttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     3601 taccgggccc cccctcgagg aattcggcat ggtgagcaag ggcgaggagc tgttcaccgg
     3661 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3721 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3781 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg
     3841 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3901 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3961 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4021 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4081 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     4141 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4201 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     4261 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccctcgagaa
     4321 agagaaagag cggccagaga tttctctccc ttcagatttt gaacacacaa ttcatgtcgg
     4381 ttttgatgct gtcacagggg agtttacggg aatgccagag cagtgggccc gcttgcttca
     4441 gacatcaaat atcactaagt cggagcagaa gaaaaacccg caggctgttc tggatgtgtt
     4501 ggagttttac aactcgaaga agacatccaa cagccagaaa tacatgagct ttacagataa
     4561 gtcagcttcc ggaggtggaa ccggtggtgg aggtaccatg caggccatca agtgtgtggt
     4621 ggtgggagac ggagctgtag gtaaaacttg cctactgatc agttacacaa ccaatgcatt
     4681 tcctggagaa tatatcccta ctgtctttga caattattct gccaatgtta tggtagatgg
     4741 aaaaccggtg aatctgggct tatgggatac agctggacaa gaagattatg acagattacg
     4801 ccccctatcc tatccgcaaa cagatgtgtt cttaatttgc ttttcccttg tgagtcctgc
     4861 atcatttgaa aatgtccgtg caaagtggta tcctgaggtg cggcaccact gtcccaacac
     4921 tcccatcatc ctagtgggaa ctaaacttga tcttagggat gataaagaca cgatcgagaa
     4981 actgaaggag aagaagctga ctcccatcac ctatccgcag ggtctagcca tggctaagga
     5041 gattggtgct gtaaaatacc tggagtgctc ggcgctcaca cagcgaggcc tcaagacagt
     5101 gtttgacgaa gcgatccgag cagtccgcgg ccgcatggtg agcaagggcg aggagctgtt
     5161 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     5221 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     5281 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
     5341 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5401 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5461 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5521 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca
     5581 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     5641 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5701 cggcgacggc cccgtgctgc tgcccgacaa ccactacttg agcacccagt ccgccctgag
     5761 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5821 gatcactctc ggcatggacg agctgggccg ctctagaaag atgagcaaag atggtaaaaa
     5881 gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa gcggccgcgg tggagctcca
     5941 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagcacc
     6001 cagctttctt gtacaaagtt gagatctata attcactggc cgtcgtttta cggtaccatc
     6061 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6121 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6181 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6241 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     6301 tatgatcctc tagatccctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
     6361 tagaggtctc tcaggtttaa aaaaagcacc gactcggtgc cactttttca agttgataac
     6421 ggactagcct tatttaaact tgctatgctg tttccagcat agctcttaaa cagagacggt
     6481 cgacagtctg cagtgtcgtc tctcgaacta ggagcctgga gaactgctat ataaacccgg
     6541 gacttctggg tatgtttctg ccatgtggta agtggataaa cctacttatt caactcattt
     6601 acctcaaaca tgacagtaaa cctcttcggg gcaaacctgc aaacgcttaa aaccttcaaa
     6661 gtttgaaacc agcagttttg tagaggttta ccgtagtatt tttgaactgt aaagcatatg
     6721 cagaggaagc tggttttagg cttgtgtgat tggatgagtt cttctgtgac gtaactgatc
     6781 ttttgtcctc gtgtccttcg ccttccccca caggcatgcg cggaccttcc cccgccgcag
     6841 accagaggac aacatgctca gacgccagca aacggcccat cgaaacattc acagactctc
     6901 ggattggaga gaccgggccc gaggtctctg actaaaaaag caccgactcg gtgccacttt
     6961 ttcaagttga taacggacta gccttattta aacttgctat gctgtttcca gcatagctct
     7021 taaacagaga cggtcgacag tctgcagtgt cgtctctcga accaagagct ggagggagag
     7081 ctatatatac cagggacttc tgggtatgtt tttgggaggt ggtgagtgac taaaccactt
     7141 attcagctcc cttagatcaa gtctgaccct atatcatggt gacataaacc tgcaaactga
     7201 taaaacctga aggatctcaa atccagagtt tgtgtgaggg attaccgtgg taattttcaa
     7261 actgtaaagc atatgcaaaa ttatctggtc ttggcttgag tgattgggtg tctcggtgtg
     7321 atgcagggac gttttcagtg acgtgtcctt ctccctcccc cacaggcatg cgcagaacat
     7381 ttcccccctc cttgaagacc agaacaaaag acgccgagag caggaaactc gtcttactga
     7441 atgaccgagg ctggagaaat cgaccctagg caactttatt atacatagtt gataattcac
     7501 tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata cattgatgag
     7561 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     7621 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     7681 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     7741 ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga agatacggcc
     7801 acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat
     7861 cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt
     7921 caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga
     7981 acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat
     8041 ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga
     8101 atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa
     8161 aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt
     8221 gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat
     8281 tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc
     8341 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     8401 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8461 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8521 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8581 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8641 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9241 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9481 gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9541 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10561 ttccccgaaa agtg
//
