LOCUS       sequence-356961-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Dominant negative control of the assay to rescue Rac expression in
            neutrophil specific knockout"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-Rac-DN(guide
            resistant)-2a-mcherry-U...
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3520..3544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3546..3562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3572..3591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3572..3590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3620..3629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3626..4333
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3771..3789)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4039..4058
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4343..4399
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(5009..5027)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(5056..5080)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5093..5109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5125..5259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6503..6523)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6531..6547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6563..6697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7136..7323,7324..7335)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7369..7385)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7369..7385)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7422..7440)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7461..7477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7474..7496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7485..7501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7509..7515,7516..7533,7534..7539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7554..7575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7692..7709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7863..8451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7943..7962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8622..9413,9414..9482))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9245..9264
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9483..9587)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt
     2161 atgtgaacat ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa
     2221 accacttgtg actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa
     2281 gtactcttga atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta
     2341 taatttgtaa caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc
     2401 tgtaaatatt taggttattt atttattaat acaatataga aagtaacaaa acacaatgta
     2461 aaaaatcaat aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat
     2521 tagcaacaaa gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct
     2581 gtattaaata atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt
     2641 actggttgtt aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat
     2701 aaaggccatg tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga
     2761 gattttacaa tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa
     2821 ttacccaact ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata
     2881 ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta
     2941 ccagtatcta tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat
     3001 ataatggatt gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt
     3061 aacaaaaaat aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg
     3121 ctttatgtac actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt
     3181 ttctgaattc tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca
     3241 tcatgagctc gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa
     3301 tcactttctc ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt
     3361 cagttattgc ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa
     3421 cacaaacctt tctctggact tataaataaa ggcattcagc agctccacca tacacctcca
     3481 ctggtcttga taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca
     3541 ggcttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     3601 taccgggccc cccctcgagg ccaccatggt gagcaagggc gaggaggata acatggccat
     3661 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3721 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3781 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3841 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3901 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3961 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     4021 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     4081 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     4141 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     4201 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     4261 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     4321 cgagctgtac aagggatctg gagccacgaa cttctctctg ttaaagcaag caggagacgt
     4381 ggaagaaaac cccggtccta tgcaagcaat aaagtgtgtg gtggtcggag atggagctgt
     4441 gggaaagacc tgtcttctca tcagctacac taccaatgcg ttccccgggg agtacattcc
     4501 cacagtgttt gataactact ctgcaaatgt aatggtggat agcaaaccgg taaatctagg
     4561 cctatggaac accgcaggcc aagaagatta tgacagactg cggccactct cctacccgca
     4621 gacggatgtg tttcttatct gtttctcttt ggtgagccca gcatcattcg aaaatgtcag
     4681 agccaagtgg tacccagagg tgaggcatca ctgcccttcc actccaatta tcctggttgg
     4741 caccaagctt gacttgagag atgagaagga gaccatcgag aagctgaagg agaagaaact
     4801 ggcaccgatc acttatccgc aaggacttgc cttagcaaag gaaatagatg cagtaaaata
     4861 cctggagtgt tcggccctca ctcagagagg gctaaaaaca gtgtttgatg aggcgattcg
     4921 cgctgtgctc tgcccacagc ccaccaaggt caagaagaag ggctgcgtga tgctctaagc
     4981 ggccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5041 aatcatggtc atagcaccca gctttcttgt acaaagttga gatctataat tcactggccg
     5101 tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga tgagtttgga
     5161 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     5221 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     5281 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     5341 aaatgtggta tggctgatta tgatcctcta gatcccttgg gtagcagcga agtcgaggca
     5401 tttctgtcct ggctggtcta gaggtctctc aggtttaaaa aaagcaccga ctcggtgcca
     5461 ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgtt tccagcatag
     5521 ctcttaaaca cagggtctcg cattggcccg aactaggagc ctggagaact gctatataaa
     5581 cccgggactt ctgggtatgt ttctgccatg tggtaagtgg ataaacctac ttattcaact
     5641 catttacctc aaacatgaca gtaaacctct tcggggcaaa cctgcaaacg cttaaaacct
     5701 tcaaagtttg aaaccagcag ttttgtagag gtttaccgta gtatttttga actgtaaagc
     5761 atatgcagag gaagctggtt ttaggcttgt gtgattggat gagttcttct gtgacgtaac
     5821 tgatcttttg tcctcgtgtc cttcgccttc ccccacaggc atgcgcggac cttcccccgc
     5881 cgcagaccag aggacaacat gctcagacgc cagcaaacgg cccatcgaaa cattcacaga
     5941 ctctcggatt ggagagaccg ggcccgaggt ctctgactaa aaaagcaccg actcggtgcc
     6001 actttttcaa gttgataacg gactagcctt atttaaactt gctatgctgt ttccagcata
     6061 gctcttaaac gggactctgg gatacagccc gaaccaagag ctggagggag agctatatat
     6121 accagggact tctgggtatg tttttgggag gtggtgagtg actaaaccac ttattcagct
     6181 cccttagatc aagtctgacc ctatatcatg gtgacataaa cctgcaaact gataaaacct
     6241 gaaggatctc aaatccagag tttgtgtgag ggattaccgt ggtaattttc aaactgtaaa
     6301 gcatatgcaa aattatctgg tcttggcttg agtgattggg tgtctcggtg tgatgcaggg
     6361 acgttttcag tgacgtgtcc ttctccctcc cccacaggca tgcgcagaac atttcccccc
     6421 tccttgaaga ccagaacaaa agacgccgag agcaggaaac tcgtcttact gaatgaccga
     6481 ggctggagaa atcgacccta ggcaacttta ttatacatag ttgataattc actggccgtc
     6541 gttttacggt accatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     6601 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6661 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6721 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6781 atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc
     6841 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
     6901 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
     6961 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
     7021 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
     7081 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
     7141 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
     7201 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     7261 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     7321 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     7381 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     7441 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7501 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7561 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7621 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7681 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7741 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8281 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8401 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8461 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg
     8521 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8581 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8641 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8701 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8761 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8821 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8881 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8941 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9001 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9061 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9121 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9181 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9241 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9301 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9361 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9421 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9481 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9541 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9601 aaagtg
//
