LOCUS       sequence-356992-         414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds 
            SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity
            purification, allowing gentle pH or temperature release..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, 
            Howarth M
  TITLE     SpySwitch enables pH- or heat-responsive capture and release for 
            plug-and-display nanoassembly.
  JOURNAL   Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8.
  PUBMED    35764623
REFERENCE   2  (bases 1 to 414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-28"; volume: "13(1):3714. doi"; pages: " 
            10.1038/s41467-022-31193-8"
COMMENT     Sequence Label: SpySwitch
FEATURES             Location/Qualifiers
     source          1..414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ggtagcggcg gtagcggcgc catggtaacc
       61 accttatcag gtttatcagg tgagcaaggt ccgtccggtg atatgacaac tgaagaagat
      121 agtgctaccc atattcactt ccataaacat gatgaggacg gccgtgagtt agctggtgca
      181 actatggagt tgcgtgattg ctctggtaaa actattagta catggattac agatggacat
      241 gtgaaggatt tctacctgta tccaggaaaa tatacatttg tcgcgaccgc agcaccagac
      301 ggtcatcacg tagcaactcc aattgaattt acaattaatg aggacggtca ggttactgta
      361 gatggtgaag caactgaagg tgaccctcat actggatcca gtggtagcta ataa
//
