LOCUS       sequence-357006-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag 
            enables covalent reaction with SpyCatcher..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, 
            Howarth M
  TITLE     SpySwitch enables pH- or heat-responsive capture and release for 
            plug-and-display nanoassembly.
  JOURNAL   Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8.
  PUBMED    35764623
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-28"; volume: "13(1):3714. doi"; pages: " 
            10.1038/s41467-022-31193-8"
COMMENT     Sequence Label: SpyTag-sfGFP
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..42
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             82..792
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             811..828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctcata tcgtcatggt tgacgcgtat aaaccgacca aaggatcaga aaacctgtat
       61 tttcagggag gtggttcagg ccgtaaaggc gaagagctgt tcactggtgt cgtccctatt
      121 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
      181 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
      241 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
      301 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
      361 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
      421 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
      481 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
      541 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
      601 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
      661 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
      721 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
      781 gaactgtaca aaggctccgg ctccggctcc caccatcacc atcaccattg ataa
//
