LOCUS       sequence-357010-        5850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC24A1_STOP
FEATURES             Location/Qualifiers
     source          1..5850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3407..3506)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3523..3542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3524..3542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3547..3563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3547..3563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3665..3684)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3676..4485
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3743..3762)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4632..5220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5121..5140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5374..5391
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5550..5577)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5669..5755)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5804..5826
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5818..5835
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5819..5835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg caagctgatc
      121 cggatgggcc cccaggagag atggctgctg aggacaaagc gcctgcactg gtccagactg
      181 ctgttcctgc tgggcatgct gatcatcggc tctacctatc agcacctgcg gagacccaga
      241 ggactgagct ccctgtgggc agccgtgtct agccaccagc ctatcaagct ggcctctagg
      301 gacctgtcct ctgaggagat gatgatgatg agctcctctc ctagcaagcc aagctccgag
      361 atgggcggca agatgctggt gcctcaggca agcgtgggat ccgatgaggc caccctgtcc
      421 atgacagtgg agaacatccc ctctatgcct aagaggaccg ccaagatgat ccctaccaca
      481 accaagaaca attactcccc aaccgcagca ggaacagaga ggaggaagga ggacacacca
      541 acctctagcc ggacactgac ctactataca agcacctcct ctagacagat cgtgaagaag
      601 tacacaccca cccctagggg cgagatgaag tcttatagcc ccacccaggt gagggagaag
      661 gtgaagtaca caccatcccc aaggggccgg agagtgggaa catatgtgcc tagcacattc
      721 atgaccatgg agacatccca cgccatcacc ccacgcacaa ccgtgaagga cagcgatatc
      781 acagccacct acaagatcct ggagaccaac tccctgaagc ggatcatgga ggagacaacc
      841 cccacaaccc tgaagggcat gttcgacagc acacctacct ttctgaccca cgaggtggag
      901 gccaatgtgc tgacatctcc aagaagcgtg atggagaaga acaatctgtt cccccctagg
      961 cgcgtggagt ctaacagctc cgcccaccca tggggactgg tgggcaagag caatccaaag
     1021 accccacagg gaacagtgct gctgcacacc cctgccacat ctgagggcca ggtgaccatc
     1081 agcacaatga ccggctctag cccagccgag accaaggcct ttacagccgc ctggtccctg
     1141 aggaaccctt ctccacgcac ctccgtgtct gccatcaaga cagcaccagc aatcgtgtgg
     1201 aggctggcca agaagcctag caccgcacca agcacatcca caacccctac cgtgcgggcc
     1261 aagctgacaa tgcaggtgca ccactgcgtg gtggtgaagc ccacccctgc catgctgaca
     1321 accccatctc ccagcctgac aaccgccctg ctgccagagg agctgtcccc ttctccaagc
     1381 gtgctgccac catccctgcc agacctgcac cctaagggag agtatcctcc agatctgttc
     1441 tctgtggagg agcggagaca gggctgggtg gtgctgcacg tgttcggcat gatgtacgtg
     1501 ttcgtggccc tggccatcgt gtgcgacgag tacttcgtgc ctgccctggg cgtgatcacc
     1561 gacaagctgc agatcagcga ggatgtggca ggagccacct tcatggcagc aggaggctcc
     1621 gccccagagc tgttcacctc tctgatcggc gtgtttatct cccactctaa cgtgggaatc
     1681 ggaaccatcg tgggatccgc cgtgttcaat atcctgtttg tgatcggcac ctgttccctg
     1741 ttttctcgcg agatcctgaa cctgacatgg tggcccctgt tccgggacgt gagcttttat
     1801 atcctggatc tgatcatgct gatcctgttc tttctggata gcctgatcgc ctggtgggag
     1861 tccctgctgc tgctgctggc ctacgccttc tacgtgttca ccatgaaatg gaataagcac
     1921 atcgaagtgt gggtgaagga gcagctgtcc aggcgcccag tggccaaagt gatggccctg
     1981 gaggacctgt ctaagccagg cgatggagca atcgcagtgg acgagctgca ggataacaag
     2041 aagctgaagc tgccctccct gctgaccaga ggctcctcta gcacaagcct gcacaattcc
     2101 accatcaggt ctacaatcta ccagctgatg ctgcacagcc tggaccctct gcgggaggtg
     2161 agactggcca aggagaaaga ggaggagagc ctgaaccagg gagcaagggc acagccacag
     2221 gcaaaggcag agtccaagcc agaggaggag gagccagcaa agctgccagc agtgacagtg
     2281 accccagcac ctgtgccaga catcaagggc gatcagaagg agaatccagg aggacaggag
     2341 gatgtggcag aggcagagtc tacaggagag atgccaggag aggagggaga gaccgcagga
     2401 gagggcgaga cagaggagaa gagcggagga gagacccagc ctgagggaga gggagagaca
     2461 gagacccagg gcaagggaga ggagtgcgag gacgagaacg aggccgaggg caagggcgac
     2521 aatgagggcg aggatgaggg cgagatccac gccgaggatg gcgagatgaa gggcaacgag
     2581 ggagagaccg agagccagga gctgtccgcc gagaaccacg gagaggccaa gaatgacgag
     2641 aagggagtgg aggatggagg aggaagcgac ggaggcgatt ccgaggaaga agaggaggag
     2701 gaggaggagc aggaagagga agaagaagaa gaggagcagg aagaggagga agaagaagag
     2761 gaagaagaag aggaaaaggg caatgaggag ccactgtccc tggactggcc tgagacccgc
     2821 cagaagcagg ccatctatct gttcctgctg cctatcgtgt ttccactgtg gctgacagtg
     2881 cctgatgtgc ggagacagga gagccggaag ttcttcgtgt tcaccttcct gggctctatc
     2941 atgtggatcg ccatgttcag ctacctgatg gtgtggtggg cacaccaagt gggagagaca
     3001 atcggcatca gcgaggagat catgggactg accatcctgg cagcaggcac atctatccca
     3061 gacctgatca ccagcgtgat cgtggcaagg aagggactgg gcgacatggc cgtgtcctct
     3121 agcgtgggca gcaacatctt cgatatcacc gtgggactgc cagtgccttg gctgctgttt
     3181 tccctgatca acggcctgca gccagtgccc gtgtcctcta atggcctgtt ttgcgccatc
     3241 gtgctgctgt tcctgatgct gctgtttgtg atcagctcca tcgcctcctg taagtggaga
     3301 atgaataaga tcctgggctt caccatgttt ctgctgtact tcgtgtttct gatcatctct
     3361 gtgatgctgg aggataggat catctcttgt cccgtgagcg tgtaagaccc agctttcttg
     3421 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     3481 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     3541 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     3601 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     3661 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     3721 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     3781 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     3841 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     3901 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     3961 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     4021 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     4081 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     4141 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     4201 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     4261 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     4321 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     4381 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     4441 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     4501 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     4561 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     4621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5221 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5341 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5401 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5461 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     5521 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     5581 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     5641 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     5701 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     5761 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     5821 aaaacgacgg ccagtcttaa gctcgggccc
//
