LOCUS       sequence-357013-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg
            residues removed, two Lys removed, three Asp added (LCD: 
            low-complexity domain, aa 186-320).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Bremer A, Farag M, Borcherds WM, Peran I, Martin EW, Pappu RV, 
            Mittag T
  TITLE     Deciphering how naturally occurring sequence features impact the 
            phase behaviours of disordered prion-like domains.
  JOURNAL   Nat Chem. 2022 Feb;14(2):196-207. doi: 10.1038/s41557-021-00840-w. 
            Epub 2021 Dec 20.
  PUBMED    34931046
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-021-00840-w"; journalName: "Nat Chem"; date: 
            "2022-02"; volume: "14"; issue: "2"; pages: "196-207"
COMMENT     Sequence Label: A1-LCD-2R-2K+3D
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaga acctgtattt tcagggtagc atggcaagcg caagcagcag ccaggatggt
      121 cgtagcggta gcggtaattt tggtggtggt cgcggtggtg gttttggtgg caatgataat
      181 tttggccgtg gtggtaattt tagcggtcgt ggtggctttg gcggtagccg tggcggtggc
      241 ggttatggcg gtagtggtga tggttataat ggctttggta atgatggtag caactttgga
      301 ggtggtggta gctataatga tttcggcaat tataacaacc agagcagcaa ttttggtccg
      361 atggatggtg gtaacttcgg tggtcgtagt agcggtccgt atggtggcgg aggtcagtat
      421 tttgcagatc cgcgtaatca aggtggctat ggtggttcta gcagcagcag ctcatatggt
      481 tcaggtggtc gtttttaa
//
