LOCUS       sequence-357020-        7884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7884)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Vinculin-N-21
FEATURES             Location/Qualifiers
     source          1..7884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3807..4487
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     polyA_signal    4612..4733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4649..4668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4703..4722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4740..5195)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4877..4898)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5089..5108
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5222..5326
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5328..5685
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5328..5519
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5351..5371)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5536..5671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5720..6514
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5720..6514
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5774..5793)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6384..6403
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6702..6721)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6746..6793
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7122..7710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7611..7630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      541 ccaccatgcc agtgtttcat acgcgcacga tcgagagcat cctggagccg gtggcacagc
      601 agatctccca cctggtgata atgcacgagg agggcgaggt ggacggcaaa gccattcctg
      661 acctcaccgc gcccgtggcc gccgtgcagg cggccgtcag caacctcgtc cgggttggaa
      721 aagagactgt tcaaaccact gaggatcaga ttttgaagag agatatgcca ccagcattta
      781 ttaaggttga gaatgcttgc accaagcttg tccaggcagc tcagatgctt cagtcagacc
      841 cttactcagt gcctgctcga gattatctaa ttgatgggtc aaggggcatc ctctctggaa
      901 catcagacct gctccttacc ttcgatgagg ctgaggtccg taaaattatt agagtttgca
      961 aaggaatttt ggaatatctt acagtggcag aggtggtgga gactatggaa gatttggtca
     1021 cttacacaaa gaatcttggg ccaggaatga ctaagatggc caagatgatt gacgagagac
     1081 agcaggagct cactcaccag gagcaccgag tgatgttggt gaactcgatg aacaccgtga
     1141 aagagttgct gccagttctc atttcagcta tgaagatttt tgtaacaact aaaaactcaa
     1201 aaaaccaagg catagaggaa gctttaaaaa atcgcaattt tactctagaa aaaatgagtg
     1261 ctgaaattaa tgagataatt cgtgtgttac aactcacctc ttgggatgaa gatgcctggg
     1321 ccagcaagga cactgaagcc atgaagagag cattggcctc catagactcc aaactgaacc
     1381 aggccaaagg ttggctccgt gaccctagtg cctccccagg ggatgctggt gagcaggcca
     1441 tcagacagat cttagatgaa gctggaaaag ttggtgaact ctgtgcaggc aaagaacgca
     1501 gggagattct gggaacttgc aaaatgctag ggcagatgac tgatcaagtg gctgacctcc
     1561 gtgccagagg acaaggatcc tcaccggtgg ccatgcagaa agctcagcag gtatctcagg
     1621 gtctggatgt gctcacagca aaagtggaaa atgcagctcg caagctggaa gccatgacca
     1681 actcaaagca gagcattgca aagaagatcg atgctgctca gaactggctt gcagatccaa
     1741 atggtggacc ggaaggagaa gagcagattc gaggtgcttt ggctgaagct cggaaaatag
     1801 cagaattatg tgatgatcct aaagaaagag atgacattct acgttccctt ggggaaatat
     1861 ctgctctgac ttctaaatta gcagatctac gaagacaggg gaaaggagat tctccagagg
     1921 ctcgagcctt ggccaaacag gtggccacgg ccctgcagaa cctgcagacc aaaaccaacc
     1981 gggctgtggc caacagcaga ccggccaaag cagctgtaca ccttgagggc aagattgagc
     2041 aagcacagcg gtggattgat aatcccacag tggatgaccg tggagtcggt caggctgcca
     2101 tccgggggct tgtggccgaa gggcatcgtc tggctaatgt tatgatgggg ccttatcggc
     2161 aagatcttct cgccaagtgt gaccgagtgg accagctgac agcccagctg gctgacctgg
     2221 ctgccagagg ggaaggggag agtcctcagg cacgagcact tgcatctcag ctccaagact
     2281 ccttaaagga tctaaaagct cggatgcagg aggccatgac tcaggaagtg tcagatgttt
     2341 tcagcgatac cacaactccc atcaagctgt tggcagtggc agccacggcg cctcctgatg
     2401 cgcctaacag ggaagaggta tttgatgaga gggcagctaa ctttgaaaac cattcaggaa
     2461 agcttggtgc tacggccgag aaggcggctg cggttggtac tgctaataaa tcaacagtgg
     2521 aaggcattca ggcctcagtg aagacggccc gagaactcac accccaggtg gtctcggctg
     2581 ctcgtatctt acttaggaac cctggaaatc aagctgctta tgaacatttt gagaccatga
     2641 agaaccagtg gatcgataat gttgaaaaaa tgacagggct ggtggacgaa gccattgata
     2701 ccaaatctct gttggatgct tcagaagaag caattaaaaa agacctggac aagtgcaagg
     2761 tagctatggc caacattcag cctcagatgc tggttgctgg ggcaaccagt attgctcgtc
     2821 gggccaaccg gatcctgctg gtggctaaga gggaggtgga aaattccgag gatcccaagt
     2881 tccgtgaggc tgtgaaagct gcctctgatg aattgagcaa aaccatctcc ccgatggtga
     2941 tggatgcaaa agctgtggct ggaaacattt ccgaccctgg actgcaaaag agcttcctgg
     3001 actcaggata tcggatcctg ggagctgtgg ccaaggtcag agaagccttc caacctcagg
     3061 agcctgactt cccgccgcct ccaccagacc ttgaacaact ccgactaaca gatgagcttg
     3121 ctcctcccaa accacctctg cctgaaggtg aggtccctcc acctaggcct ccaccaccag
     3181 aggaaaagga tgaagagttc cctgagcaga aggccgggga ggtgattaac cagccaatga
     3241 tgatggctgc cagacagctc catgatgaag ctcgcaaatg gtccagcaag ggcaatgaca
     3301 tcattgcagc agccaagcgc atggctctgc tgatggctga gatgtctcgg ctggtaagag
     3361 ggggcagtgg taccaagcgg gcactcattc agtgtgccaa ggacatcgcc aaggcctcag
     3421 atgaggtgac tcggttggcc aaggaggttg ccaagcagtg cacagataaa cggattagaa
     3481 ccaacctctt acaggtatgt gagcgaatcc caaccataag cacccagctc aaaatcctgt
     3541 ccacagtgaa ggccaccatg ctgggccgga ccaacatcag tgatgaggag tctgagcagg
     3601 ccacagagat gctggttcac aatgcccaga acctcatgca gtctgtgaag gagactgtgc
     3661 gggaagctga agctgcttca atcaaaattc gaacagatgc tggatttaca ctgcgctggg
     3721 ttagaaagac tccctggtac cagtccggtg gcagcggaat tctgcagtcg acggtaccgc
     3781 gggcccggga tccaccggtc gccaccatga acagcctgat caaagaaaac atgcggatga
     3841 aggtggtgct ggaaggcagc gtgaacggcc accagttcaa gtgcaccggc gagggcgagg
     3901 gcaaccccta catgggcacc cagaccatgc ggatcaaagt gatcgagggc ggacctctgc
     3961 ccttcgcctt cgacatcctg gccacatcct tcatgtacgg cagccggacc ttcatcaagt
     4021 accccaaggg catccccgat ttcttcaagc agagcttccc cgagggcttc acctgggaga
     4081 gagtgaccag atacgaggac ggcggcgtga tcaccgtgat gcaggacacc agcctggaag
     4141 atggctgcct ggtgtaccat gcccaggtca ggggcgtgaa ttttcccagc aacggcgccg
     4201 tgatgcagaa gaaaaccaag ggctgggagc ccaacaccga gatgatgtac cccgctgacg
     4261 gcggactgag aggctacacc cacatggccc tgaaggtgga cggcggaggg cacctgagct
     4321 gcagcttcgt gaccacctac cgatccaaga aaaccgtggg caacatcaag atgcccggca
     4381 tccacgccgt ggaccaccgg ctggaaaggc tggaagagtc cgacaacgag atgttcgtgg
     4441 tgcagcggga gcacgccgtg gccaagttcg ccggcctggg cggagggtaa gcggccgcga
     4501 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     4561 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     4621 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4681 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     4741 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     4801 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     4861 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     4921 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     4981 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5041 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     5101 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     5161 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     5221 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5281 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     5341 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5401 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5461 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5521 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5581 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5641 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     5701 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5761 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5821 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5881 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5941 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6001 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6061 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6121 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6181 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6241 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6301 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6361 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6421 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6481 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6541 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6601 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6661 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     6721 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     6781 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6841 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     6901 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     6961 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7021 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7261 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7501 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7741 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7801 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     7861 tcatagccca tatatggagt tccg
//
