LOCUS       sequence-357022-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged A1-LCD with seven 
            Arg residues added, twelve Glu residues added (LCD: low-complexity 
            domain, aa 186-320).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Bremer A, Farag M, Borcherds WM, Peran I, Martin EW, Pappu RV, 
            Mittag T
  TITLE     Deciphering how naturally occurring sequence features impact the 
            phase behaviours of disordered prion-like domains.
  JOURNAL   Nat Chem. 2022 Feb;14(2):196-207. doi: 10.1038/s41557-021-00840-w. 
            Epub 2021 Dec 20.
  PUBMED    34931046
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-021-00840-w"; journalName: "Nat Chem"; date: 
            "2022-02"; volume: "14"; issue: "2"; pages: "196-207"
COMMENT     Sequence Label: A1-LCD+7R+12D
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..54
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    232..256
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctggaagtgc tgtttcaggg cccggatacc
       61 tataaactgg tgattgtgct gaacggcacc acctttacct ataccaccga agcggtggat
      121 gcggcgaccg cggaaaaagt gtttaaacag tatgcgaacg atgcgggcgt ggatggcgaa
      181 tggacctatg atgcggcgac caaaaccttt accgtgaccg aactcgaatc aacaagtttg
      241 tacaaaaaag caggcttcga gaacctgtat tttcagggta gcatggcaag cgcagatagc
      301 agccagcgtg atcgtgatga tcgtggtaat tttggtgatg gtcgcggtgg tggttttggt
      361 ggtaatgata attttggccg tggtggcaat tttagcgatc gcggtggctt tggcggtagc
      421 cgtggtgatg gccgttatgg cggtgatggt gatcgttata atggttttgg caatgacggt
      481 cgcaactttg gtggtggtgg cagctataac gattttggca actataataa ccagagcagc
      541 aacttcgatc cgatgaaagg cggtaacttt cgtgatcgta gcagcggtcc gtatgaccgt
      601 ggtggtcagt attttgccaa accgcgtaat caaggtggct atggtggtag tagcagcagc
      661 cgtagctatg gtagcgatcg tcgtttttaa
//
