LOCUS       sequence-357029-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression and purification. Encodes ataxin-3 
            Josephin domain fused with His-Tag (N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Figueiredo F, Lopes-Marques M, Almeida B, Matscheko N, Martins PM, 
            Silva A, Macedo-Ribeiro S
  TITLE     A Robust Assay to Monitor Ataxin-3 Amyloid Fibril Assembly.
  JOURNAL   Cells. 2022 Jun 19;11(12). pii: cells11121969. doi: 
            10.3390/cells11121969.
  PUBMED    35741099
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-06-19"; volume: "11(12). pii: cells11121969. doi"; 
            pages: " 10.3390/cells11121969"
COMMENT     Sequence Label: ATX3-JD
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggctggatgg agtccatctt ccacgagaaa caagaaggct cactttgtgc tcaacattgc
      121 ctgaataact tattgcaagg agaatatttt agccctgtgg aattatcctc aattgcacat
      181 cagctggatg aggaggagag gatgagaatg gcagaaggag gagttactag tgaagattat
      241 cgcacgtttt tacagcagcc ttctggaaat atggatgaca gtggtttttt ctctattcag
      301 gttataagca atgccttgaa agtttggggt ttagaactaa tcctgttcaa cagtccagag
      361 tatcagaggc tcaggatcga tcctataaat gaaagatcat ttatatgcaa ttataaggaa
      421 cactggttta cagttagaaa attaggaaaa cagtggttta acttgaattc tctcttgacg
      481 ggtccagaat taatatcaga tacatatctt gcacttttct tggctcaatt acaacaggaa
      541 ggttattcta tatttgtcgt taagggtgat ctgccagatt gcgaagctga ccaactccta
      601 cagatgatta ggtga
//
