LOCUS       sequence-357038-        1841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG tagged FZD5 swapped with FZD5 CRDlinker domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ko SB, Mihara E, Park Y, Roh K, Kang C, Takagi J, Bang I, Choi HJ
  TITLE     Functional role of the Frizzled linker domain in the Wnt signaling 
            pathway.
  JOURNAL   Commun Biol. 2022 May 5;5(1):421. doi: 10.1038/s42003-022-03370-4.
  PUBMED    35513706
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-05-5"; volume: "5(1):421. doi"; pages: " 
            10.1038/s42003-022-03370-4"
COMMENT     Sequence Label: pcDNA FZD4_5CRDlinker
FEATURES             Location/Qualifiers
     source          1..1841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             127..171
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             172..195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1804..1821
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cccatttggg gagccaatat aaggcagagc tctctggcta actagagaac ccactgctta
       61 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agttaagctt
      121 ggtaccatgt ctgcacttct gatcctagct cttgttggag ctgcagttgc tgactacaaa
      181 gacgatgacg acaagcttgc ggccgcagcg tccaaggccc cggtgtgcca ggaaatcacg
      241 gtgcccatgt gccgcggcat cggctacaac ctgacgcaca tgcccaacca gttcaaccac
      301 gacacgcagg acgaggcggg cctggaggtg caccagttct ggccgctggt ggagatccaa
      361 tgctcgccgg acctgcgctt cttcctatgc tctatgtaca cgcccatctg tctgcccgac
      421 taccacaagc cgctgccgcc ctgccgctcg gtgtgcgagc gcgccaaggc cggctgctcg
      481 ccgctgatgc gccagtacgg cttcgcctgg cccgagcgca tgagctgcga ccgcctcccg
      541 gtgctgggcc gcgacgccga ggtcctctgc atggattaca accgcagcga ggccaccacg
      601 gcgcccccca ggcctttccc agccaagccc acccttccag gcccgccagg ggcgccggcc
      661 tcggggggcg aatgccccgc tgggggcccg ttcgtgtgca agtgtcgcga gcccttcgtg
      721 cccattctga aggagtcaca cccgctctac aacaaggtgc ggacgggcca ggtgcccaac
      781 tgcgcggtac cctgtggcta cgatgctggc ttgtacagcc gctcagctaa ggagttcacg
      841 gatatttgga tggctgtgtg ggccagcctc tgcttcatct ccaccacctt caccgtgctg
      901 accttcctga ttgattcatc caggttttct taccctgagc gccccatcat atttctcagt
      961 atgtgctata atatttatag cattgcttat attgttcggc tgactgtagg ccgggaaagg
     1021 atatcctgtg attttgaaga ggcggcagag cccgttctca tccaagaagg acttaagaac
     1081 acaggatgtg caataatttt cttgctgatg tacttttttg gaatggccag ctccatttgg
     1141 tgggttattc tgacactcac ttggtttttg gcagccggac tcaagtgggg tcatgaagcc
     1201 attgaaatgc acagttctta tttccacatc gcagcctggg ctattcccgc agtgaaaacc
     1261 attgtcatct tgattatgag actagtggat gccgatgaac tgactggctt gtgctatgtt
     1321 gggaaccaaa acctagatgc cctcactggc tttgtggtgg ctcctctctt tacgtatttg
     1381 gtgattggaa cgctgttcat tgcggcgggt ttggtggcct tattcaaaat tcggtccaat
     1441 cttcaaaaag acgggacaaa gacagacaag ttggaaaggc taatggtcaa gatcggggtc
     1501 ttctcagtac tgtacacggt tcctgcaacc tgtgtgattg cctgttattt ctatgaaatc
     1561 tcaaactggg cactctttcg atattctgca gatgactcaa acatggcagt tgaaatgttg
     1621 aaaattttta tgtctttgct cgtgggcatc acttcaggca tgtggatttg gtctgccaaa
     1681 actcttcaca cgtggcaaaa gtgttctaac cgattggtga attctgggaa ggtaaagaga
     1741 gagaagaggg ggaatggttg ggtgaagcca ggaaaaggca acgagactgt ggtatagctc
     1801 gagcatcatc accatcacca tgagttaacc ccctgatccc c
//
