LOCUS       sequence-357046-        1332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein expression and purification. Encodes ataxin-3 
            full length with 3 UIMs and 77Q in the Poly-Q track fused with 
            His-Tag and TEV cleavage sequence (N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Figueiredo F, Lopes-Marques M, Almeida B, Matscheko N, Martins PM, 
            Silva A, Macedo-Ribeiro S
  TITLE     A Robust Assay to Monitor Ataxin-3 Amyloid Fibril Assembly.
  JOURNAL   Cells. 2022 Jun 19;11(12). pii: cells11121969. doi: 
            10.3390/cells11121969.
  PUBMED    35741099
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-06-19"; volume: "11(12). pii: cells11121969. doi"; 
            pages: " 10.3390/cells11121969"
COMMENT     Sequence Label: ATX3-77Q
FEATURES             Location/Qualifiers
     source          1..1332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaaaacc tgtattttca gggcatggag
       61 tccatcttcc acgagaaaca agaaggctca ctttgtgctc aacattgcct gaataactta
      121 ttgcaaggag aatattttag ccctgtggaa ttatcctcaa ttgcacatca gctggatgag
      181 gaggagagga tgagaatggc agaaggagga gttactagtg aagattatcg cacgttttta
      241 cagcagcctt ctggaaatat ggatgacagt ggttttttct ctattcaggt tataagcaat
      301 gccttgaaag tttggggttt agaactaatc ctgttcaaca gtccagagta tcagaggctc
      361 aggatcgatc ctataaatga aagatcattt atatgcaatt ataaggaaca ctggtttaca
      421 gttagaaaat taggaaaaca gtggtttaac ttgaattctc tcttgacggg tccagaatta
      481 atatcagata catatcttgc acttttcttg gctcaattac aacaggaagg ttattctata
      541 tttgtcgtta agggtgatct gccagattgc gaagctgacc aactcctgca gatgattagg
      601 gtccaacaga tgcatcgacc aaaactgatt ggcgaagaac tggctcaact gaaagaacag
      661 cgtgtgcata agaccgacct ggaacgtgtc ctggaagcaa atgacggcag cggcatgctg
      721 gatgaagacg aagaagatct gcagcgtgcc ctggcactgt ctcgtcagga aattgatatg
      781 gaagacgaag aagcagatct gcgtcgcgct attcagctgt caatgcaggg cagctctcgt
      841 aacatctcgc aggacatgac ccagacgagc ggtaccaatc tgacgtctga agaactgcgc
      901 aaacgtcgcg aagcatattt tgaaaaacag caacagaagc aacaacagca gcaacaacaa
      961 caacaacagc agcagcagca gcaacaacaa cagcaacaac aacagcaaca gcagcaacag
     1021 caacagcaac agcagcaaca acagcaacag caacaacagc aacagcaaca acagcaacaa
     1081 cagcaacaac agcaacaaca gcaacaacag caacaacagc aacagcaaca acagcagcag
     1141 caacagcagc aacaacaaca gagagatctg tcaggccaga gttcccatcc gtgtgaacgt
     1201 ccggccacct caagcggtgc actgggtagt gatctgggtg acgccatgtc cgaagaagac
     1261 atgctgcagg cagcagtgac gatgtccctg gaaaccgtgc gtaacgacct gaaaaccgaa
     1321 ggcaaaaaat aa
//
