LOCUS       sequence-357056-       10940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express Flag-GluN2A from a TRE3G 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..10940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1956..2334
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1964..1982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2000..2018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2072..2090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2243..2267
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2344..2353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2512..2535
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6793..7381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6846..6866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7264..7275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7461..7694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7766..7887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7803..7822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7857..7876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7927..8062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8082..8101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8083..8101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8111..8128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8111..8127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8120..8142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8269..8724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8356..8375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8566..8587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8750..8854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8855..8923,8924..9715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9073..9092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9886..10474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10375..10394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10628..10645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10762..10783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10798..10803,10804..10821,10822..10828)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10836..10852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10841..10863
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10860..10876
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10860..10876
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10895..10915
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10897..10915
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatacg cgtatcacga gactagcctc gagttggctt tactccctat cagtgataga
     1981 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     2041 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     2101 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     2161 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     2221 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     2281 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaagaattc
     2341 gccgccacca tgggcagatt gggctactgg accttgctgg tattgccggc ccttctggtc
     2401 tggcgcgatc cggcgcagaa cgcggcggcg gagaagggtc ctccagcgct gaacattgcg
     2461 gtgctgctgg gtcacagcca cgacgtgaca gaacgcgaac ttcgaaatct ggactacaag
     2521 gacgacgatg acaagtgggg cccagagcag gcaaccggct tgcccctgga tgtgaacgtg
     2581 gtggcgttat tgatgaaccg cactgaccct aagagcctca tcacgcatgt gtgcgacctc
     2641 atgtccgggg cgcgcatcca cggcttggtg tttggagatg acacggacca ggaggctgtg
     2701 gcccagatgc tggattttat ctcctcacag acttttatcc ccatcttggg cattcatggg
     2761 ggtgcatcta tgatcatggc tgacaaggat ccgacatcca cgttcttcca gtttggagcc
     2821 tccatccagc agcaagccac agttatgctg aagatcatgc aggactacga ctggcacgtc
     2881 ttctccctgg tcaccaccat cttccctggc taccgagact tcatcagctt tatcaagaca
     2941 acagtggaca acagctttgt gggctgggat atgcagaacg tgatcacact ggacacctcc
     3001 ttcgaggacg ccaagacgca ggtccagctg aagaagatcc attcttctgt catcctgctc
     3061 tactgctcca aagacgaggc tgtcctcatc ctgagcgagg ctcgctccct cggcctcact
     3121 ggctatgatt tcttctggat tgtccccagt ttggtgtctg ggaacacaga gctcatcccc
     3181 aaagagtttc catcaggtct catttcagtc tcttatgacg actgggacta cagcctggag
     3241 gcaagagtga gagacggtct tgggatctta accactgccg catcctccat gttggagaag
     3301 ttctcctaca ttcctgaggc caaggccagc tgctatgggc aggcagagaa gccagagacc
     3361 ccgctacaca ccctgcacca attcatggtc aatgtgactt gggatggcaa ggacttgtcc
     3421 ttcactgagg aaggttacca ggtgcacccc aggcttgtgg tgatcgtgct gaacaaggac
     3481 cgggagtggg aaaaggtggg caagtgggag aaccagacgc tgagcctgag gcacgctgtg
     3541 tggccaaggt acaagtcctt ttctgactgc gagccagatg acaaccacct cagcattgtc
     3601 accttggagg aagccccctt cgtcatcgta gaggacatag accccctgac tgagacctgt
     3661 gtgaggaaca cggtgccctg tcggaagttc gtcaagatca acaattcaac caacgaaggg
     3721 atgaatgtga agaaatgctg caaggggttc tgcatcgaca tcctcaagaa gctgtccaga
     3781 actgtgaagt tcacctatga cctctacctg gtgaccaatg ggaagcatgg gaaaaaggtt
     3841 aacaatgtgt ggaatggaat gataggtgaa gtggtctatc aacgagcagt catggctgtg
     3901 ggctcactca ccatcaatga ggagcgttcg gaagtggtgg acttctcggt gcccttcgtg
     3961 gagacaggaa tcagcgtcat ggtctccagg agtaatggca ctgtctcccc ttctgctttc
     4021 ctcgaaccct tcagtgcctc cgtctgggtg atgatgtttg tgatgctgct catcgtctca
     4081 gccattgctg tcttcgtttt tgaatacttc agtcctgttg gatacaacag aaacttagcc
     4141 aaagggaaag ctccccacgg gccttctttt actattggaa aagctatatg gctcctctgg
     4201 ggcctggtct tcaacaattc tgtgcctgtc cagaatccta aaggcacaac cagcaagatc
     4261 atggtgtcag tgtgggcctt ctttgctgtc atcttcctgg ccagttacac agccaacctg
     4321 gctgccttca tgatccagga ggagtttgtg gaccaagtga ctggcctcag tgacaagaag
     4381 ttccagagac ctcatgacta ttctccacct ttccgatttg ggacggtacc caatggaagt
     4441 acagagagga atattcgtaa caactacccc tatatgcacc agtacatgac cagattcaac
     4501 cagaggggag tggaggatgc cttggtcagc ttgaaaaccg ggaagttgga cgctttcatc
     4561 tatgacgcag ccgtcttgaa ctacaaggcc gggagggatg aaggctgtaa actggtgacc
     4621 attgggagcg ggtacatctt tgctaccaca ggctatggaa ttgcgctgca gaagggctca
     4681 ccctggaaga ggcagattga cctcgctctg ctccagtttg ttggtgatgg tgagatggag
     4741 gagctggaga cactgtggct tacgggcatc tgccacaacg agaagaatga ggtgatgagt
     4801 agccagctgg acatcgataa catggcgggc gtgttctaca tgctggctgc agccatggcc
     4861 ctcagcctca tcaccttcat ctgggagcac ctcttctact ggaagctgcg cttctgcttc
     4921 acaggcgtgt gctctgaccg gcccgggctg ctcttctcca tcagcagggg catctatagt
     4981 tgcatccatg gggtacacat tgaagaaaag aagaaatctc cagatttcaa tctgactgga
     5041 tcacagagca acatgctaaa gcttcttcgg tcagctaaaa acatctccaa tatgtccaac
     5101 atgaactcct caagaatgga ctcacccaaa agagctactg acttcattca aagagggtca
     5161 cttattgtgg acatggtttc agacaaggga aatttgatat attcagacaa cagatccttt
     5221 caagggaagg acagtatatt tggagacaac atgaatgaac tccaaacatt tgtggccaac
     5281 aggcacaagg ataatctcag taactatgtg tttcaaggac agcatcctct cactctcaat
     5341 gagtccaacc ctaacacagt agaggtggct gtcagcactg aatccaaagg gaactcccga
     5401 ccccggcagc tttggaagaa atccatggag tctctacgcc aggattctct aaaccagaac
     5461 ccagtctccc agagggatga gaagactgca gagaatcgga cccactcgct aaagagtcct
     5521 aggtatcttc cagaagaggt agcccactct gacatttcag aaacctcaag ccgggccaca
     5581 tgccacaggg agccagataa caataagaac cacaagacca aggataactt caaacggtca
     5641 atggcctcta agtatcccaa ggactgtagc gatgttgacc gcacctacat gaaaaccaaa
     5701 gcaagttctc ccagggataa gatctatacc attgatggtg agaaggagcc cagcttccac
     5761 ttagatcctc ctcagtttgt tgagaatata accctgcctg agaatgtggg cttcccagat
     5821 acctaccaag atcacaatga gaacttccgc aagggggact ccacactgcc catgaacagg
     5881 aacccattac ataatgaaga cgggcttccc aacaatgacc aatataaact ctatgccaag
     5941 cactttacct tgaaagacaa gggttcccca cacagtgagg gcagtgatcg ataccggcag
     6001 aactccacac attgcagaag ctgcctttcg aatctgccca cctactcagg ccactttacc
     6061 atgaggtctc ctttcaagtg tgatgcctgt ctgcggatgg ggaatctcta tgacattgat
     6121 gaagaccaga tgcttcagga gacaggtaac ccagctactc gggaggaggt ctaccagcag
     6181 gactggtcac agaacaacgc cctccagttc cagaagaaca agctaaggat taaccgacag
     6241 cactcctatg ataacattct ggacaaaccc agagagatag accttagcag gccctcccgg
     6301 agcataagcc tcaaggacag ggaacggcta ctggagggca acttgtatgg gagcctgttc
     6361 agtgtcccct caagcaaact cttggggaac aaaagctccc ttttccccca aggtctggag
     6421 gacagcaaga ggagcaagtc tctcttgcca gaccacgcct ctgataatcc tttcctccac
     6481 acgtatgggg atgaccaacg cttagttatc gggagatgtc cctcggaccc ttacaaacac
     6541 tcattgccat cacaggcggt aaatgacagc tatcttcggt catccttgag gtcaacagca
     6601 tcatattgct ccagggacag tcggggccac agtgatgtgt atatttcaga gcatgttatg
     6661 ccttatgctg caaataagaa taccatgtac tctaccccca gggttttaaa ttcctgcagc
     6721 aatagacgag tgtacaagaa aatgcctagt atcgaatctg atgtttaact cgaccggtta
     6781 gtaatgatcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7201 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7381 cctggcgatg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7441 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7681 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7741 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7861 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7921 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7981 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8041 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     8101 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8161 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8221 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     8281 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     8341 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     8401 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     8461 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     8521 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8581 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8641 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8701 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8821 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8881 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8941 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9001 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9061 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     9121 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9181 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9241 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9301 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9361 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9421 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9481 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9541 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9601 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9661 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9721 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9781 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9841 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9901 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9961 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10021 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    10081 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    10141 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10201 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10261 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10321 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10381 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10441 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10501 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10561 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10621 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    10681 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    10741 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    10801 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    10861 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
    10921 aaagctggag ctgcaagctt
//
