LOCUS       sequence-357058-       11216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express Flag-GluN2B from a CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA
FEATURES             Location/Qualifiers
     source          1..11216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1943..2246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2447..2471
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2699..2722
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7069..7657
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7122..7142)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7540..7551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7737..7970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8042..8163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8079..8098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8133..8152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8203..8338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8358..8377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8359..8377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8387..8404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8387..8403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8396..8418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8545..9000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8632..8651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8842..8863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9026..9130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9131..9199,9200..9991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9349..9368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10162..10750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10651..10670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10904..10921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11038..11059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11074..11079,11080..11097,11098..11104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11112..11128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11117..11139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11136..11152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11136..11152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11171..11191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11173..11191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatacg cgtggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     2461 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgag
     2521 atctggatcc gccgccacca tgaagcccag cgcagagtgc tgttctccca agttctggtt
     2581 ggtgttggcc gtcttggccg tatcgggcag caaagctcgt tcccaaaaga gcgcccccag
     2641 catcggcatc gctgtcatcc tcgtgggcac ttccgacgaa gtggccataa aagatgccga
     2701 ctacaaggac gacgatgaca agcacgagaa agatgacttc catcatctct cagtagttcc
     2761 ccgggtggag ctggtagcca tgaacgagac tgacccaaag agcataatca cccgcatctg
     2821 cgatcttatg tctgaccgga agatccaggg ggtggtgctc gcggatgaca cggaccagga
     2881 agccatcgcc cagatcctcg atttcatttc tgctcagact ctcaccccca tcctgggcat
     2941 ccatgggggc tcatctatga taatggcaga taaggatgag tcctccatgt tcttccagtt
     3001 tggcccatcc attgaacagc aagcttctgt catgctcaac atcatggaag aatacgactg
     3061 gtacatcttc tccatcgtca ccacctactt ccccggctac caggacttcg tgaacaagat
     3121 ccgcagcact attgagaaca gctttgtagg ctgggagctc gaggaagtcc tcctgctaga
     3181 catgtctcta gacgatggcg actctaagat tcagaatcag ctgaagaagc tgcagagccc
     3241 catcattctc ctctactgca caaaggaaga agccacctac atcttcgaag tagctaactc
     3301 agttgggctg actggctacg gctacacatg gatcgtgccg agtctggtgg cgggggatac
     3361 agacacggtg ccttcagagt tccccacggg gctcatctct gtgtcatatg acgaatggga
     3421 ctatggcctt cctgccagag tgagagatgg gattgccatc atcaccactg ctgcctcgga
     3481 catgctgtcc gaacacagtt tcatccctga gcccaagagc agttgctaca acacccacga
     3541 gaagaggatc taccagtcta acatgctgaa taggtatctg atcaacgtca cttttgaagg
     3601 gagaaacctg tccttcagtg aagatggcta ccagatgcat ccgaagctgg tgataatcct
     3661 tctgaacaag gagaggaagt gggagagggt gggaaaatgg aaagacaagt ccctgcagat
     3721 gaaatactac gtgtggcctc gaatgtgtcc agagactgaa gaacaggaag atgaccatct
     3781 gagcatcgtt accttggagg aggcaccgtt tgtcattgtg gaaagtgtgg accctctcag
     3841 tgggacctgc atgcggaata cagtcccgtg ccagaagcgc atcatctctg agaataaaac
     3901 agatgaggaa ccaggctaca tcaaaaaatg ctgcaagggg ttttgtattg atatccttaa
     3961 gaaaatttct aagtctgtga agttcaccta tgacctttac ctggtgacca atggcaagca
     4021 tggaaagaaa atcaacggga cctggaacgg catgattggt gaggtggtca tgaagagggc
     4081 ctacatggca gtgggatcac taactatcaa tgaagaacgg tcagaggtgg ttgacttctc
     4141 tgtgcccttc atagagactg gcatcagtgt catggtatca cgcagcaatg ggactgtgtc
     4201 accttctgcc ttcttagagc cattcagtgc tgacgtgtgg gtgatgatgt ttgtgatgct
     4261 gctcattgtc tctgctgtag ctgtctttgt ctttgaatac ttcagccctg tgggttacaa
     4321 ccggtgccta gctgatggca gagagccagg cggcccatct ttcaccatcg gcaaagcgat
     4381 ttggttactc tggggtctgg tgtttaacaa ctccgtacct gtgcagaacc caaaggggac
     4441 cacctccaag atcatggtgt cagtgtgggc cttctttgct gtcattttcc tggccagcta
     4501 cactgccaac ttagccgcct tcatgatcca agaggagtat gtggaccagg tttccggcct
     4561 gagtgacaag aagttccaga gacctaatga cttctcaccc cctttccgct ttgggactgt
     4621 gcccaatggc agcacagaga ggaatatccg taataactat gcagaaatgc atgcctacat
     4681 gggaaagttc aaccaaaggg gtgtagatga tgccttgctc tccctgaaaa cagggaaact
     4741 tgatgcattc atctacgatg cagccgtgct caactacatg gctggaagag acgaaggctg
     4801 caagctggtg accattggca gtggcaaggt ctttgcttct acgggctatg gcattgctat
     4861 ccaaaaagac tctggttgga aacgccaggt ggaccttgct atcctgcagc tgtttggaga
     4921 tggggagatg gaagaactgg aagctctctg gctcactggc atttgccaca atgagaagaa
     4981 tgaggttatg agcagccagc tggacattga caacatggcg ggcgtcttct atatgttggg
     5041 ggcagccatg gctctcagcc tcatcacctt catctgtgaa catctcttct attggcagtt
     5101 ccgacattgc ttcatgggtg tctgttctgg caagcctggc atggtcttct ccatcagcag
     5161 aggtatctac agctgtatcc acggagtagc tatagaggag cgccaatccg tgatgaactc
     5221 ccccactgcc accatgaaca acacacactc caatatccta cgcttgctcc gaacggccaa
     5281 aaacatggcc aacctgtctg gagtcaacgg ctccccccag agtgccctgg acttcatccg
     5341 ccgtgagtcc tctgtctatg acatctctga gcatcgccgc agcttcacgc attcagactg
     5401 caagtcgtac aataacccac cctgtgagga aaacctgttc agtgactaca ttagtgaggt
     5461 agagagaaca tttggcaacc tgcagctgaa ggacagcaat gtgtaccaag accactatca
     5521 ccatcaccac cggccccaca gcatcggcag caccagctcc attgatgggc tctatgactg
     5581 tgacaaccca ccctttacca cccagcccag gtcaatcagc aagaaacccc tggacattgg
     5641 cctgccctcc tccaaacaca gccagctcag cgacctgtac ggcaagttct ctttcaagag
     5701 tgaccgctac agtggccatg atgacttgat tcgatcggat gtctcagaca tctccacgca
     5761 tactgtcacc tatggcaaca tcgagggcaa cgcagccaag aggaggaagc agcaatataa
     5821 ggacagtcta aagaagcggc cagcctcggc caaatctagg agggagtttg atgaaatcga
     5881 gctggcctac cgtcgccgac caccccgctc cccagaccac aagcgctact tcagggacaa
     5941 agaagggctc cgagacttct acctggacca gttccgaaca aaggagaact cgcctcactg
     6001 ggagcacgtg gacttaactg acatttacaa agaacgtagt gatgacttca agcgagattc
     6061 ggtcagtgga ggcgggccct gtaccaacag gtctcacctt aaacacggaa caggcgataa
     6121 gcacggagtg gtaggcgggg tgcctgctcc ttgggagaag aacctgacca atgtggattg
     6181 ggaggatagg tctgggggca acttctgccg cagctgtccc tccaagctgc acaattactc
     6241 ctctacggtg gcagggcaaa actcgggccg gcaggcctgc atcaggtgtg aggcctgcaa
     6301 gaaggctggc aacctgtatg acatcagcga ggacaactcc ctgcaggaac tggaccagcc
     6361 ggctgcccct gtggctgtgt catccaacgc ctccaccacc aagtaccctc aaagcccgac
     6421 taattccaag gcccagaaga agaatcggaa caaactgcgc cggcagcact cctacgacac
     6481 cttcgtggac ctgcagaagg aggaggccgc cttggcccca cgcagcgtga gcctgaaaga
     6541 caagggccga ttcatggatg ggagccccta cgcccatatg tttgagatgc cagctggtga
     6601 gagctccttt gccaacaagt cctcagtgac cactgccgga caccatcaca acaatcccgg
     6661 cagcggctac atgctcagca agtcgctcta ccctgaccgg gtcacgcaaa accctttcat
     6721 ccccactttt ggggatgatc agtgcttgct tcacggcagc aaatcctact tcttcaggca
     6781 gcccacggtg gcaggggcgt cgaaaacaag gccggacttc cgggcccttg tcaccaataa
     6841 gccagtggtg tcggcccttc atggggctgt gccaggtcgt ttccagaagg acatttgtat
     6901 agggaaccag tccaacccct gtgtgcctaa caacaaaaac cccagggctt tcaatggctc
     6961 cagcaatgga catgtttatg agaaactttc tagtattgag tctgatgtct gagtgagggg
     7021 aattcagatc tggatccggt acccgcgtac cggttagtaa tgatcgacaa tcaacctctg
     7081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7201 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7261 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7321 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7441 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7501 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7561 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7621 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gcgatggtac ctttaagacc
     7681 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7741 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7921 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7981 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8041 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8101 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8161 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8221 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8281 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8341 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8401 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8461 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8521 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8581 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8641 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8701 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8761 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8821 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8881 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8941 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9001 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9061 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    11041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    11101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    11161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagctt
//
