LOCUS       sequence-357060-       11161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express Flag-GluN2A from a CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 11161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..11161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1943..2246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2447..2471
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2528..2537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2696..2719
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    7014..7602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7067..7087)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7485..7496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7682..7915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7987..8108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8024..8043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8078..8097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8148..8283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8303..8322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8304..8322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8332..8349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8332..8348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8341..8363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8490..8945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8577..8596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8787..8808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8971..9075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9076..9144,9145..9936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9294..9313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10107..10695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10596..10615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10849..10866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10983..11004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11019..11024,11025..11042,11043..11049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11057..11073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11062..11084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11081..11097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11081..11097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11116..11136
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11118..11136
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatacg cgtggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac agtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     2461 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgga
     2521 attcgccgcc accatgggca gattgggcta ctggaccttg ctggtattgc cggcccttct
     2581 ggtctggcgc gatccggcgc agaacgcggc ggcggagaag ggtcctccag cgctgaacat
     2641 tgcggtgctg ctgggtcaca gccacgacgt gacagaacgc gaacttcgaa atctggacta
     2701 caaggacgac gatgacaagt ggggcccaga gcaggcaacc ggcttgcccc tggatgtgaa
     2761 cgtggtggcg ttattgatga accgcactga ccctaagagc ctcatcacgc atgtgtgcga
     2821 cctcatgtcc ggggcgcgca tccacggctt ggtgtttgga gatgacacgg accaggaggc
     2881 tgtggcccag atgctggatt ttatctcctc acagactttt atccccatct tgggcattca
     2941 tgggggtgca tctatgatca tggctgacaa ggatccgaca tccacgttct tccagtttgg
     3001 agcctccatc cagcagcaag ccacagttat gctgaagatc atgcaggact acgactggca
     3061 cgtcttctcc ctggtcacca ccatcttccc tggctaccga gacttcatca gctttatcaa
     3121 gacaacagtg gacaacagct ttgtgggctg ggatatgcag aacgtgatca cactggacac
     3181 ctccttcgag gacgccaaga cgcaggtcca gctgaagaag atccattctt ctgtcatcct
     3241 gctctactgc tccaaagacg aggctgtcct catcctgagc gaggctcgct ccctcggcct
     3301 cactggctat gatttcttct ggattgtccc cagtttggtg tctgggaaca cagagctcat
     3361 ccccaaagag tttccatcag gtctcatttc agtctcttat gacgactggg actacagcct
     3421 ggaggcaaga gtgagagacg gtcttgggat cttaaccact gccgcatcct ccatgttgga
     3481 gaagttctcc tacattcctg aggccaaggc cagctgctat gggcaggcag agaagccaga
     3541 gaccccgcta cacaccctgc accaattcat ggtcaatgtg acttgggatg gcaaggactt
     3601 gtccttcact gaggaaggtt accaggtgca ccccaggctt gtggtgatcg tgctgaacaa
     3661 ggaccgggag tgggaaaagg tgggcaagtg ggagaaccag acgctgagcc tgaggcacgc
     3721 tgtgtggcca aggtacaagt ccttttctga ctgcgagcca gatgacaacc acctcagcat
     3781 tgtcaccttg gaggaagccc ccttcgtcat cgtagaggac atagaccccc tgactgagac
     3841 ctgtgtgagg aacacggtgc cctgtcggaa gttcgtcaag atcaacaatt caaccaacga
     3901 agggatgaat gtgaagaaat gctgcaaggg gttctgcatc gacatcctca agaagctgtc
     3961 cagaactgtg aagttcacct atgacctcta cctggtgacc aatgggaagc atgggaaaaa
     4021 ggttaacaat gtgtggaatg gaatgatagg tgaagtggtc tatcaacgag cagtcatggc
     4081 tgtgggctca ctcaccatca atgaggagcg ttcggaagtg gtggacttct cggtgccctt
     4141 cgtggagaca ggaatcagcg tcatggtctc caggagtaat ggcactgtct ccccttctgc
     4201 tttcctcgaa cccttcagtg cctccgtctg ggtgatgatg tttgtgatgc tgctcatcgt
     4261 ctcagccatt gctgtcttcg tttttgaata cttcagtcct gttggataca acagaaactt
     4321 agccaaaggg aaagctcccc acgggccttc ttttactatt ggaaaagcta tatggctcct
     4381 ctggggcctg gtcttcaaca attctgtgcc tgtccagaat cctaaaggca caaccagcaa
     4441 gatcatggtg tcagtgtggg ccttctttgc tgtcatcttc ctggccagtt acacagccaa
     4501 cctggctgcc ttcatgatcc aggaggagtt tgtggaccaa gtgactggcc tcagtgacaa
     4561 gaagttccag agacctcatg actattctcc acctttccga tttgggacgg tacccaatgg
     4621 aagtacagag aggaatattc gtaacaacta cccctatatg caccagtaca tgaccagatt
     4681 caaccagagg ggagtggagg atgccttggt cagcttgaaa accgggaagt tggacgcttt
     4741 catctatgac gcagccgtct tgaactacaa ggccgggagg gatgaaggct gtaaactggt
     4801 gaccattggg agcgggtaca tctttgctac cacaggctat ggaattgcgc tgcagaaggg
     4861 ctcaccctgg aagaggcaga ttgacctcgc tctgctccag tttgttggtg atggtgagat
     4921 ggaggagctg gagacactgt ggcttacggg catctgccac aacgagaaga atgaggtgat
     4981 gagtagccag ctggacatcg ataacatggc gggcgtgttc tacatgctgg ctgcagccat
     5041 ggccctcagc ctcatcacct tcatctggga gcacctcttc tactggaagc tgcgcttctg
     5101 cttcacaggc gtgtgctctg accggcccgg gctgctcttc tccatcagca ggggcatcta
     5161 tagttgcatc catggggtac acattgaaga aaagaagaaa tctccagatt tcaatctgac
     5221 tggatcacag agcaacatgc taaagcttct tcggtcagct aaaaacatct ccaatatgtc
     5281 caacatgaac tcctcaagaa tggactcacc caaaagagct actgacttca ttcaaagagg
     5341 gtcacttatt gtggacatgg tttcagacaa gggaaatttg atatattcag acaacagatc
     5401 ctttcaaggg aaggacagta tatttggaga caacatgaat gaactccaaa catttgtggc
     5461 caacaggcac aaggataatc tcagtaacta tgtgtttcaa ggacagcatc ctctcactct
     5521 caatgagtcc aaccctaaca cagtagaggt ggctgtcagc actgaatcca aagggaactc
     5581 ccgaccccgg cagctttgga agaaatccat ggagtctcta cgccaggatt ctctaaacca
     5641 gaacccagtc tcccagaggg atgagaagac tgcagagaat cggacccact cgctaaagag
     5701 tcctaggtat cttccagaag aggtagccca ctctgacatt tcagaaacct caagccgggc
     5761 cacatgccac agggagccag ataacaataa gaaccacaag accaaggata acttcaaacg
     5821 gtcaatggcc tctaagtatc ccaaggactg tagcgatgtt gaccgcacct acatgaaaac
     5881 caaagcaagt tctcccaggg ataagatcta taccattgat ggtgagaagg agcccagctt
     5941 ccacttagat cctcctcagt ttgttgagaa tataaccctg cctgagaatg tgggcttccc
     6001 agatacctac caagatcaca atgagaactt ccgcaagggg gactccacac tgcccatgaa
     6061 caggaaccca ttacataatg aagacgggct tcccaacaat gaccaatata aactctatgc
     6121 caagcacttt accttgaaag acaagggttc cccacacagt gagggcagtg atcgataccg
     6181 gcagaactcc acacattgca gaagctgcct ttcgaatctg cccacctact caggccactt
     6241 taccatgagg tctcctttca agtgtgatgc ctgtctgcgg atggggaatc tctatgacat
     6301 tgatgaagac cagatgcttc aggagacagg taacccagct actcgggagg aggtctacca
     6361 gcaggactgg tcacagaaca acgccctcca gttccagaag aacaagctaa ggattaaccg
     6421 acagcactcc tatgataaca ttctggacaa acccagagag atagacctta gcaggccctc
     6481 ccggagcata agcctcaagg acagggaacg gctactggag ggcaacttgt atgggagcct
     6541 gttcagtgtc ccctcaagca aactcttggg gaacaaaagc tcccttttcc cccaaggtct
     6601 ggaggacagc aagaggagca agtctctctt gccagaccac gcctctgata atcctttcct
     6661 ccacacgtat ggggatgacc aacgcttagt tatcgggaga tgtccctcgg acccttacaa
     6721 acactcattg ccatcacagg cggtaaatga cagctatctt cggtcatcct tgaggtcaac
     6781 agcatcatat tgctccaggg acagtcgggg ccacagtgat gtgtatattt cagagcatgt
     6841 tatgccttat gctgcaaata agaataccat gtactctacc cccagggttt taaattcctg
     6901 cagcaataga cgagtgtaca agaaaatgcc tagtatcgaa tctgatgttt aactcgagaa
     6961 gcttgaattc agatctggat ccggtacccg cgtaccggtt agtaatgatc gacaatcaac
     7021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7381 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggcgat ggtaccttta
     7621 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7681 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7741 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7801 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7861 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7921 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7981 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8041 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8101 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     8161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8281 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8461 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8521 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8581 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8641 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8701 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8761 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8821 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8881 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8941 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9061 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9121 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9181 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9241 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9301 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9361 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9421 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9481 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9541 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9601 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9661 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9721 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9781 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9841 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9901 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9961 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10021 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10801 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10861 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10921 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10981 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    11041 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    11101 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
    11161 t
//
