LOCUS       sequence-357061-       12016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express GFP tagged GluN2A from a 
            CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 12016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..12016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2596..2605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2683..2685,2686..3396)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2683..2685,2686..3396)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2725..2746)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2986..3005)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3333..3354
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7727..7751)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7804..7845
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7869..8457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7922..7942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8340..8351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8537..8770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8842..8963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8879..8898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8933..8952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9003..9138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9158..9177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9159..9177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9187..9204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9187..9203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9196..9218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9345..9800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9432..9451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9642..9663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9826..9930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9931..9999,10000..10791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10149..10168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10962..11550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11451..11470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11704..11721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11838..11859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11874..11879,11880..11897,11898..11904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11912..11928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11917..11939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11936..11952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11936..11952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11971..11991
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11973..11991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg ctgccgccac catgggcaga ttgggctact ggaccttgct ggtattgccg
     2641 gcccttctgg tctggcgcga tccggcgcag aacgcggcta gggtgagcaa gggcgaggag
     2701 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2761 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2821 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2881 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2941 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3001 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3061 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3121 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3181 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3241 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3301 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3361 gccgggatca ctctcggcat ggacgagctg tacaaggcta gcgcggcgga gaagggtcct
     3421 ccagcgctga acattgcggt gctgctgggt cacagccacg acgtgacaga acgcgaactt
     3481 cgaaatctgt ggggcccaga gcaggcaacc ggcttgcccc tggatgtgaa cgtggtggcg
     3541 ttattgatga accgcactga ccctaagagc ctcatcacgc atgtgtgcga cctcatgtcc
     3601 ggggcgcgca tccacggctt ggtgtttgga gatgacacgg accaggaggc tgtggcccag
     3661 atgctggatt ttatctcctc acagactttt atccccatct tgggcattca tgggggtgca
     3721 tctatgatca tggctgacaa ggatccgaca tccacgttct tccagtttgg agcctccatc
     3781 cagcagcaag ccacagttat gctgaagatc atgcaggact acgactggca cgtcttctcc
     3841 ctggtcacca ccatcttccc tggctaccga gacttcatca gctttatcaa gacaacagtg
     3901 gacaacagct ttgtgggctg ggatatgcag aacgtgatca cactggacac ctccttcgag
     3961 gacgccaaga cgcaggtcca gctgaagaag atccattctt ctgtcatcct gctctactgc
     4021 tccaaagacg aggctgtcct catcctgagc gaggctcgct ccttcggcct cactggctat
     4081 gatttcttct ggattgtccc cagtttggtg tctgggaaca cagagctcat ccccaaagag
     4141 tttccatcag gtctcatttc agtctcttat gacgactggg actacagcct ggaggcaaga
     4201 gtgagagacg gtcttgggat cttaaccact gccgcatcct ccatgttgga gaagttctcc
     4261 tacattcctg aggccaaggc cagctgctat gggcaggcag agaagccaga gaccccgcta
     4321 cacaccctgc accaattcat ggtcaatgtg acttgggatg gcaaggactt gtccttcact
     4381 gaggaaggtt accaggtgca ccccaggctt gtggtgatcg tgctgaacaa ggaccgggag
     4441 tgggaaaagg tgggcaagtg ggagaaccag acgctgagcc tgaggcacgc tgtgtggcca
     4501 aggtacaagt ccttttctga ctgcgagcca gatgacaacc acctcagcat tgtcaccttg
     4561 gaggaagccc ccttcgtcat cgtagaggac atagaccccc tgactgagac ctgtgtgagg
     4621 aacacggtgc cctgtcggaa gttcgtcaag atcaacaatt caaccaacga agggatgaat
     4681 gtgaagaaat gctgcaaggg gttctgcatc gacatcctca agaagctgtc cagaactgtg
     4741 aagttcacct atgacctcta cctggtgacc aatgggaagc atgggaaaaa ggttaacaat
     4801 gtgtggaatg gaatgatagg tgaagtggtc tatcaacgag cagtcatggc tgtgggctca
     4861 ctcaccatca atgaggagcg ttcggaagtg gtggacttct cggtgccctt tgtggagaca
     4921 ggaatcagcg tcatggtctc caggagtaat ggcactgtct ccccttctgc tttcctcgaa
     4981 cccttcagtg cctccgtctg ggtgatgatg tttgtgatgc tgctcatcgt ctcagccatt
     5041 gctgtcttcg tttttgaata cttcagtcct gttggataca acagaaactt agccaaaggg
     5101 aaagctcccc acgggccttc ttttactatt ggaaaagcta tatggctcct ctggggcctg
     5161 gtcttcaaca attctgtgcc tgtccagaat cctaaaggca caaccagcaa gatcatggtg
     5221 tcagtgtggg ccttctttgc tgtcatcttc ctggccagtt acacagccaa cctggctgcc
     5281 ttcatgatcc aggaggagtt tgtggaccaa gtgactggcc tcagtgacaa gaagttccag
     5341 agacctcatg actattctcc acctttccga tttgggacgg tacccaatgg aagtacagag
     5401 aggaatattc gtaacaacta cccgtatatg caccagtaca tgaccagatt caaccagagg
     5461 ggagtggagg atgccttggt cagcttgaaa accgggaagt tggacgcttt catctatgac
     5521 gcagccgtct tgaactacaa ggccgggagg gatgaaggct gtaaactggt gaccattggg
     5581 agcgggtaca tctttgctac cacaggctat ggaattgcgc tgcagaaggg ctcaccctgg
     5641 aagaggcaga ttgacctcgc tctgctccag tttgttggtg atggtgagat ggaggagctg
     5701 gagacactgt ggcttacggg catctgccac aacgagaaga atgaggtgat gagtagccag
     5761 ctggacatcg ataacatggc gggcgtgttc tacatgctgg ctgcagccat ggccctcagc
     5821 ctcatcacct tcatctggga gcacctcttc tactggaagc tgcgcttctg cttcacaggc
     5881 gtgtgctctg accggcccgg gctgctcttc tccatcagca ggggcatcta tagttgcatc
     5941 catggggtac acattgaaga aaagaagaaa tctccagatt tcaatctgac tggatcacag
     6001 agcaacatgc taaagcttct tcggtcagct aaaaacatct ccaatatgtc caacatgaac
     6061 tcctcaagaa tggactcacc taaaagagct actgacttca ttcaaagagg gtcacttatt
     6121 gtggacatgg tttcagacaa gggaaatttg atatattcag acaacagatc ctttcaaggg
     6181 aaggacagta tatttggaga caacatgaat gaactccaaa catttgtggc caacaggcac
     6241 aaggataatc tcagtaacta tgtgtttcaa ggacagcatc ctctcactct caatgagtcc
     6301 aaccctaaca cagtagaggt ggctgtcagc actgaatcca aagggaactc ccgaccccgg
     6361 cagctttgga agaaatccat ggagtctcta cgccaggatt ctctaaacca gaacccagtc
     6421 tcccagaggg atgagaagac tgcagagaat cggacccact cgctaaagag tcctaggtat
     6481 cttccagaag aggtagccca ctctgacatt tcagaaacct caagccgggc cacatgccac
     6541 agggagccag ataacaataa gaaccacaag accaaggata acttcaaacg gtcaatggcc
     6601 tctaagtatc ccaaggactg tagcgatgtt gaccgcacct acatgaaaac caaagcaagt
     6661 tctcccaggg ataagatcta taccattgat ggtgagaagg agcccagctt ccacttagat
     6721 cctcctcagt ttgttgagaa tataaccctg cctgagaatg tgggcttccc agatacctac
     6781 caagatcaca atgagaactt ccgcaagggg gactccacac tgcccatgaa caggaaccca
     6841 ttacataatg aagacgggct tcccaacaat gaccaatata aactctatgc caagcacttt
     6901 accttgaaag acaagggttc cccacacagt gagggcagtg atcgataccg gcagaactcc
     6961 acacattgca gaagctgcct ttcgaatctg cccacctact caggccactt taccatgagg
     7021 tctcctttca agtgtgatgc ctgtctgcgg atggggaatc tctatgacat tgatgaagac
     7081 cagatgcttc aggagacagg taacccagct actcgggagg aggtctacca gcaggactgg
     7141 tcacagaaca acgccctcca gttccagaag aacaagctaa ggattaaccg acagcactcc
     7201 tatgataaca ttctggacaa acccagagag atagacctta gcaggccctc ccggagcata
     7261 agcctcaagg acagggaacg gctactggag ggcaacttgt atgggagcct gttcagtgtc
     7321 ccctcaagca aactcttggg gaacaaaagc tcccttttcc cccaaggtct ggaggacagc
     7381 aagaggagca agtctctctt gccagaccac gcctccgata atcctttcct ccacacgtat
     7441 ggggatgacc aacgcttagt tatcgggaga tgtccctcgg acccttacaa acactcattg
     7501 ccatcacagg cggtaaatga cagctatctt cggtcatcct tgaggtcaac agcatcatat
     7561 tgctccaggg acagtcgggg ccacagtgat gtgtatattt cagagcatgt tatgccttat
     7621 gctgcaaata agaataccat gtactctacc cccagggttt taaattcctg cagcaataga
     7681 cgagtgtaca agaaaatgcc tagtatcgaa tctgatgttt aagatcaccc agctttcttg
     7741 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     7801 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
     7861 tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     8281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     8461 gcgatggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8521 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     8581 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8761 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     8821 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     8881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8941 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     9001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9061 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9121 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     9181 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     9241 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     9301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     9361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     9781 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10141 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
    10201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    10261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    10321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    10501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    10561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    11581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    11641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    11701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    11761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    11821 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    11881 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    11941 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
    12001 ctggagctgc aagctt
//
