LOCUS       sequence-357063-        4215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 35
            bp linker lengths in addition to carrier DNA that prevents 
            overassembly of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_35bpLinker
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      793..1380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1281..1300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1421..1442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1457..1462,1463..1480,1481..1487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1495..1511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1500..1522
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1519..1535
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1519..1535
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3981..4072
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc caatgatgag
       61 cactttcaaa gttctgctat gtggagcagt attatcccgt gtagatgcgg ggcaagagca
      121 actcggacga cggatatcct attcgcagaa tgacttggtt gaatactccc cagtgacaga
      181 aaagcacctt acggacggaa tgacggtaag agaattatgt agtgccgcca taacgatgag
      241 tgataacact gcggcgaact tacttctgac aaccatcggt ggaccgaagg aattaaccgc
      301 ttttttgcac aatatgggag accatgtaac tcgccttgac cgttgggaac cagaactgaa
      361 tgaagccata ccaaacgacg agcgagacac cacaatgcct gcggcaatgg caacaacatt
      421 acgcaaactg atatctggcg aactacttac tctggcttca cggcaacaat taatagactg
      481 gcttgaagcg gataaagttg caggaccact actgcgttcg gcacttcctg ctggctggtt
      541 tattgctgat aaatctgggg caggagagcg tggttcacgg ggtatcattg ccgcacttgg
      601 accagatggt aagccttccc gtatcgtagt tatctacacg acgggtagtc aggcaactat
      661 ggacgaacga aatagacaga ttgctgaaat aggggcttca ctgattaagc attggtaaac
      721 cgatatcatt aaaggctcct tttggagcct ttttttttgg acggaccgag tagaaaagat
      781 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      841 accaccgcta ccagcggtgg tatgtttgcc ggatcaagag ctaccaactc tttttccgaa
      901 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccggatat
      961 cggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1021 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1081 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1141 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1201 gttccgatat cgagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1261 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1321 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1381 aacgccagca acgcggcctt tttacggttc ctgaattgat taatgtgagt tagctcactc
     1441 attaggcacc ccaggcttta cactttatgc tgccggctcg tatgttgtgt ggaattgtga
     1501 gcggataaca atttcacaca ggaaacagct atgactatga ttacgcccaa gcttgatatc
     1561 atgacaggat gggaacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc
     1621 cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg
     1681 tctacgacca attgagcggc ctcggcaccg ggattctcca gttacatgat catccatcta
     1741 gatggacagg atgggaacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1801 tccccttggc ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc
     1861 tgtctacgac caattgagcg gcctcggcac cgggattctc cagttacatg atcatccagc
     1921 tagatggaca ggatgggaac aggatgtata tatctgacac gtgcctggag actagggagt
     1981 aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga
     2041 gctgtctacg accaattgag cggcctcggc accgggattc tccagttaca tgatcatcca
     2101 gctagatgga caggatggga acaggatgta tatatctgac acgtgcctgg agactaggga
     2161 gtaatcccct tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta
     2221 gagctgtcta cgaccaattg agcggcctcg gcaccgggat tctccagtta catgatcatc
     2281 cagctagatg gacaggatgg gaacaggatg tatatatctg acacgtgcct ggagactagg
     2341 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
     2401 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagt tacatgatca
     2461 tccagctaga tggacaggat gggaacagga tgtatatatc tgacacgtgc ctggagacta
     2521 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     2581 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttacatgat
     2641 catccagcta gatggacagg atgggaacag gatgtatata tctgacacgt gcctggagac
     2701 tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg
     2761 gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc cagttacatg
     2821 atcatccagc tagatggaca ggatgggaac aggatgtata tatctgacac gtgcctggag
     2881 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
     2941 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccagttaca
     3001 tgatcatcca gctagatgga caggatggga acaggatgta tatatctgac acgtgcctgg
     3061 agactaggga gtaatcccct tggcggttaa aacgcggggg acagcgcgta cgtgcgttta
     3121 agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat tctccagtta
     3181 catgatcatc cagctagatg gacaggatgg gaacaggatg tatatatctg acacgtgcct
     3241 ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt
     3301 taagcggtgc tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagt
     3361 tacatgatca tccagctaga tggacaggat gggaacagga tgtatatatc tgacacgtgc
     3421 ctggagacta gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg
     3481 tttaagcggt gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca
     3541 gttacatgat catccagcta gctggacagg atgggaacag gatgtatata tctgacacgt
     3601 gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg
     3661 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc
     3721 cagttacatg atcatcatga tatcggatcc ccgggtaccg agctcgaatt cgggcttgcc
     3781 gtcgttttac agcgacgaga ctgggaaaat cctggcgtta cccaacttaa tcgccttgcc
     3841 gcacaccccc ctttcgccag ttggcgtaat agcgaagaag cccgcactga ccgcccttcc
     3901 caacagttgc gtagtctgaa tggcgaatgg cgttaaacta gtagcccgcc taatgagcgg
     3961 gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata gatatccgct
     4021 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4081 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4141 tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg cacgagtggg
     4201 atacatagaa ctgga
//
