LOCUS       sequence-357066-        4335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 45
            bp linker lengths in addition to carrier DNA that prevents 
            overassembly of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_45bpLinker
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..27
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     24..40
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..40
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2606..2697
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3633..4220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4121..4140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4261..4282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4297..4302,4303..4320,4321..4327)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(4335,1..16)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgtgagcgga taacaatttc acacaggaaa cagctatgac tatgattacg cccaagcttg
       61 atatcatgag aagtcaggat gggaacagga tgtatatatc tgacacgtgc ctggagacta
      121 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
      181 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttacatgat
      241 caccagatcc atctagatgg agaagtcagg atgggaacag gatgtatata tctgacacgt
      301 gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg
      361 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc
      421 cagttacatg atcaccagat ccagctagat ggagaagtca ggatgggaac aggatgtata
      481 tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa cgcgggggac
      541 agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag cggcctcggc
      601 accgggattc tccagttaca tgatcaccag atccagctag atggagaagt caggatggga
      661 acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct tggcggttaa
      721 aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg
      781 agcggcctcg gcaccgggat tctccagtta catgatcacc agatccagct agatggagaa
      841 gtcaggatgg gaacaggatg tatatatctg acacgtgcct ggagactagg gagtaatccc
      901 cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
      961 tacgaccaat tgagcggcct cggcaccggg attctccagt tacatgatca ccagatccag
     1021 ctagatggag aagtcaggat gggaacagga tgtatatatc tgacacgtgc ctggagacta
     1081 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     1141 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttacatgat
     1201 caccagatcc agctagatgg agaagtcagg atgggaacag gatgtatata tctgacacgt
     1261 gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg
     1321 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc
     1381 cagttacatg atcaccagat ccagctagat ggagaagtca ggatgggaac aggatgtata
     1441 tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa cgcgggggac
     1501 agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag cggcctcggc
     1561 accgggattc tccagttaca tgatcaccag atccagctag atggagaagt caggatggga
     1621 acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct tggcggttaa
     1681 aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg
     1741 agcggcctcg gcaccgggat tctccagtta catgatcacc agatccagct agatggagaa
     1801 gtcaggatgg gaacaggatg tatatatctg acacgtgcct ggagactagg gagtaatccc
     1861 cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
     1921 tacgaccaat tgagcggcct cggcaccggg attctccagt tacatgatca ccagatccag
     1981 ctagatggag aagtcaggat gggaacagga tgtatatatc tgacacgtgc ctggagacta
     2041 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     2101 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttacatgat
     2161 caccagatcc agctagctgg agaagtcagg atgggaacag gatgtatata tctgacacgt
     2221 gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg
     2281 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc
     2341 cagttacatg atcaccagat catgatatcg gatccccggg taccgagctc gaattcgggc
     2401 ttgccgtcgt tttacagcga cgagactggg aaaatcctgg cgttacccaa cttaatcgcc
     2461 ttgccgcaca cccccctttc gccagttggc gtaatagcga agaagcccgc actgaccgcc
     2521 cttcccaaca gttgcgtagt ctgaatggcg aatggcgtta aactagtagc ccgcctaatg
     2581 agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc aaatagatat
     2641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2761 tttgctcacc cagaaacgct cgtgaaagta aaagacgcag aggaccaatt gggggcacga
     2821 gtgggataca tagaactgga cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa
     2881 gagcgttttc caatgatgag cactttcaaa gttctgctat gtggagcagt attatcccgt
     2941 gtagatgcgg ggcaagagca actcggacga cggatatcct attcgcagaa tgacttggtt
     3001 gaatactccc cagtgacaga aaagcacctt acggacggaa tgacggtaag agaattatgt
     3061 agtgccgcca taacgatgag tgataacact gcggcgaact tacttctgac aaccatcggt
     3121 ggaccgaagg aattaaccgc ttttttgcac aatatgggag accatgtaac tcgccttgac
     3181 cgttgggaac cagaactgaa tgaagccata ccaaacgacg agcgagacac cacaatgcct
     3241 gcggcaatgg caacaacatt acgcaaactg atatctggcg aactacttac tctggcttca
     3301 cggcaacaat taatagactg gcttgaagcg gataaagttg caggaccact actgcgttcg
     3361 gcacttcctg ctggctggtt tattgctgat aaatctgggg caggagagcg tggttcacgg
     3421 ggtatcattg ccgcacttgg accagatggt aagccttccc gtatcgtagt tatctacacg
     3481 acgggtagtc aggcaactat ggacgaacga aatagacaga ttgctgaaat aggggcttca
     3541 ctgattaagc attggtaaac cgatatcatt aaaggctcct tttggagcct ttttttttgg
     3601 acggaccgag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tatgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3781 cttctagtgt agccggatat cggccaccac ttcaagaact ctgtagcacc gcctacatac
     3841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4021 gagctatgag aaagcgccac gttccgatat cgagaaaggc ggacaggtat ccggtaagcg
     4081 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4141 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4201 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctgaattgat
     4261 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc tgccggctcg
     4321 tatgttgtgt ggaat
//
