LOCUS       sequence-357069-        4095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 25
            bp linker lengths in addition to carrier DNA that prevents 
            overassembly of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_25bpLinker
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8..30
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     27..43
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..43
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2369..2460
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3396..3983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3884..3903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4024..4045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4060..4065,4066..4083,4084..4090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aattgtgagc ggataacaat ttcacacagg aaacagctat gactatgatt acgcccaagc
       61 ttgatatcat gatgggaaca ggatgtatat atctgacacg tgcctggaga ctagggagta
      121 atccccttgg cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag
      181 ctgtctacga ccaattgagc ggcctcggca ccgggattct ccagttacat tccatctaga
      241 tggatgggaa caggatgtat atatctgaca cgtgcctgga gactagggag taatcccctt
      301 ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac
      361 gaccaattga gcggcctcgg caccgggatt ctccagttac attccagcta gatggatggg
      421 aacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
      481 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
      541 gagcggcctc ggcaccggga ttctccagtt acattccagc tagatggatg ggaacaggat
      601 gtatatatct gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg
      661 gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc
      721 tcggcaccgg gattctccag ttacattcca gctagatgga tgggaacagg atgtatatat
      781 ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc gggggacagc
      841 gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
      901 gggattctcc agttacattc cagctagatg gatgggaaca ggatgtatat atctgacacg
      961 tgcctggaga ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt
     1021 gcgtttaagc ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct
     1081 ccagttacat tccagctaga tggatgggaa caggatgtat atatctgaca cgtgcctgga
     1141 gactagggag taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa
     1201 gcggtgctag agctgtctac gaccaattga gcggcctcgg caccgggatt ctccagttac
     1261 attccagcta gatggatggg aacaggatgt atatatctga cacgtgcctg gagactaggg
     1321 agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct
     1381 agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccagtt acattccagc
     1441 tagatggatg ggaacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1501 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1561 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag ttacattcca gctagatgga
     1621 tgggaacagg atgtatatat ctgacacgtg cctggagact agggagtaat ccccttggcg
     1681 gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc
     1741 aattgagcgg cctcggcacc gggattctcc agttacattc cagctagatg gatgggaaca
     1801 ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg cggttaaaac
     1861 gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga ccaattgagc
     1921 ggcctcggca ccgggattct ccagttacat tccagctagc tggatgggaa caggatgtat
     1981 atatctgaca cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga
     2041 cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg
     2101 caccgggatt ctccagttac attcatgata tcggatcccc gggtaccgag ctcgaattcg
     2161 ggcttgccgt cgttttacag cgacgagact gggaaaatcc tggcgttacc caacttaatc
     2221 gccttgccgc acacccccct ttcgccagtt ggcgtaatag cgaagaagcc cgcactgacc
     2281 gcccttccca acagttgcgt agtctgaatg gcgaatggcg ttaaactagt agcccgccta
     2341 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaataga
     2401 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2461 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2521 gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca
     2581 cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct
     2641 gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc
     2701 cgtgtagatg cggggcaaga gcaactcgga cgacggatat cctattcgca gaatgacttg
     2761 gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta
     2821 tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc
     2881 ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt
     2941 gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg
     3001 cctgcggcaa tggcaacaac attacgcaaa ctgatatctg gcgaactact tactctggct
     3061 tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt
     3121 tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca
     3181 cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac
     3241 acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct
     3301 tcactgatta agcattggta aaccgatatc attaaaggct ccttttggag cctttttttt
     3361 tggacggacc gagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtatgttt gccggatcaa
     3481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3541 gttcttctag tgtagccgga tatcggccac cacttcaaga actctgtagc accgcctaca
     3601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3781 cgtgagctat gagaaagcgc cacgttccga tatcgagaaa ggcggacagg tatccggtaa
     3841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctgaatt
     4021 gattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgctgccggc
     4081 tcgtatgttg tgtgg
//
