LOCUS       sequence-357077-        9324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse full length LRRC33 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Jiang A, Qin Y, Springer TA
  TITLE     Loss of LRRC33-dependent TGFbeta1 activation enhances anti-tumor 
            immunity and checkpoint blockade therapy.
  JOURNAL   Cancer Immunol Res. 2022 Feb 18. pii: 681626. doi: 
            10.1158/2326-6066.CIR-21-0593.
  PUBMED    35181792
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Immunol Res. 2022 Feb 18. pii: 681626. doi: 
            10.1158/2326-6066.CIR-21-0593."
COMMENT     Sequence Label: pD2529-CAG-HA-mouse LRRC33
FEATURES             Location/Qualifiers
     source          1..9324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..306
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        308..583
                     /label=chicken beta-actin promoter
     intron          584..1600
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1524..1546
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1608..1627
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1785..1811
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5395..5450
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5449..5468)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(6160..6748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6240..6259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6834..7643)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7557..7576
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7662..7709)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(8135..8734)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8218..8238)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8715..8734
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8744..9243)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8792..8811)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9185..9208
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      361 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      421 gggggggcgc gcgccaggcg gggcggggsg gggsgrgggg sggggsgggg sgrggcggrg
      481 aggkgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      541 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      601 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      661 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      721 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      781 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
      841 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
      901 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
      961 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1021 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1081 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1141 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1201 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1261 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1321 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     1381 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     1441 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     1501 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1561 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1621 tattgtgctg tctcatcatt ttggcaaaga attcggcttg atcgaagccg tctcagggga
     1681 gagaccacac ccaagctgtc tagagccgcc accttgtgaa gagcgccgcc accatgcctt
     1741 tgctcctcct gttgcccttg ctctgggctg gcgccctcgc ggcctaccca tacgatgttc
     1801 cagattacgc tctcgaggct tcccaaggag gctgcaaggt ggtcgacgga gtcgctgact
     1861 gccggggtct gaaccttgct tcggtaccca gcagcctccc accccattcc cggatgctca
     1921 ttctggatgc taaccctctc aaggacctgt ggaaccattc cctccaggcc tacccacgcc
     1981 tggagaacct cagcctgcat agctgtcacc tggaccgtat cagccactac gccttccgag
     2041 agcaaggcca cttacgcaac ctggttctgg cagacaaccg cctctcggag aactacaagg
     2101 agtcagccgc agcactccac accctgctgg gacttcggag actggacttg tctggaaact
     2161 ccctgaccga agacatggcg gctctcatgc ttcagaacct ctcctcgttg gaggttgtgt
     2221 ccttggcaag gaataccctc atgaggctcg acgactctat ctttgagggc ctggaacacc
     2281 ttgtagagct agatttgcag agaaactaca tctttgagat cgagggcggg gcgtttgacg
     2341 gcttgactga gctgaggcgt ctcaaccttg cctataacaa cctcccctgc attgtggact
     2401 tcagcctcac ccagttacgg ttcctcaacg tcagttataa catcctagag tggttcctgg
     2461 cagccaggga ggaggtggcc ttcgaattgg agatactgga cctgtctcac aaccagctgc
     2521 tcttcttccc actcttgcct cagtgcggca agctgcacac cctcctgctc caggacaaca
     2581 acatgggctt ctacagggag ctttacaaca cctcctcccc gcaggagatg gtggcccagt
     2641 tccttcttgt ggacggcaat gtgactaaca tcacaactgt caacctctgg gaggagtttt
     2701 cctccagcga cctgtcagct cttcgcttcc tggacatgag ccaaaaccag ttccggcact
     2761 tgccggatgg tttcctgaag aagacacctt ccctttccca cctgaacctc aaccagaact
     2821 gcctgaagat gctccacatc cgggagcacg agccgccggg agcactcacc gagctggatc
     2881 tgagccacaa ccagttggca gagctgcacc tggctccggg gctcacgggc tccctcagga
     2941 acctccgggt gttcaacctg agttccaacc agctcctggg ggtccccact ggccttttcg
     3001 acaatgccag cagcatcact acaatcgaca tgagtcacaa tcagatctca ctttgtcctc
     3061 agatggtgcc cgtagactgg gaggggcctc ccagttgtgt ggatttcaga aacatgggct
     3121 ctttgaggag cctttctctg gacggctgtg ggctgaaggc attacaagac tgcccgttcc
     3181 aggggacctc cctcactcat ttagacctgt ctagcaactg gggggttctg aatgggagca
     3241 tcagccctct ctgggctgtt gccccgacat tacaggtcct gtctctcagg gacgtgggcc
     3301 ttggttctgg cgctgcagag atggacttct ctgcgtttgg gaatctgcgg gcgttggatc
     3361 tgtcgggaaa ctccctgacc agcttcccaa agttcaaggg cagtttggcc cttcggactc
     3421 tcgacctccg cagaaactct ctcacggccc tccctcagag ggttgtgtcc gagcagcctc
     3481 tgaggggtct gcagaccatc tacctcagcc agaaccctta tgactgctgt ggggtggaag
     3541 gatggggggc cctgcagcag cacttcaaga ctgttgcgga cttgtccatg gtcacttgca
     3601 acctctcttc caagatcgtc cgtgtggtgg agctgcccga aggcctgcct cagggctgta
     3661 agtgggaaca ggtggacact ggtctcttct acctcgtgct catcctgccc agctgcctca
     3721 ccctgctggt ggcctgtact gtcgtcttcc tcacttttaa gaagcctttg cttcaggtca
     3781 tcaagagccg ctgccactgg tcctccatat actgataagc tagcggttga ggtctctaaa
     3841 agcgtcttcc tgttctcatc acatcatatc aaggttatat accatcaata ttgccacaga
     3901 tgttacttag ccttttaata tttctctaat ttagtgtata tgcaatgata gttctctgat
     3961 ttctgagatt gagtttctca tgtgtaatga ttatttagag tttctctttc atctgttcaa
     4021 atttttgtct agttttattt tttactgatt tgtaagactt ctttttataa tctgcatatt
     4081 acaattctct ttactggggt gttgcaaata ttttctgtca ttctatggcc tgacttttct
     4141 taatggtttt ttaattttaa aaataagtct taatattcat gcaatctaat taacaatctt
     4201 ttctttgtgg ttaggacttt gagtcataag aaatttttct ctacactgaa gtcatgatgg
     4261 catgcttcta tattattttc taaaagattt aaagttttgc cttctccatt tagacttata
     4321 attcactgga atttttttgt gtgtatggta tgacatatgg gttccctttt attttttaca
     4381 tataaatata tttccctgtt tttctaaaaa agaaaaagat catcattttc ccattgtaaa
     4441 atgccatatt tttttcatag gtcacttaca tatatcaatg ggtctgtttc tgagctctac
     4501 tctattttat cagcctcact gtctatcccc acacatctca tgctttgctc taaatcttga
     4561 tatttagtgg aacattcttt cccattttgt tctacaagaa tatttttgtt attgtctttg
     4621 ggctttctat atacattttg aaatgaggtt gacaagttaa taacaggcct attgattgga
     4681 aagtttgtca acgaattgtg ggtcttttgg ggtttgctgc cccttttacg caatgtggat
     4741 atcctgcttt aatgccttta tatgcatgta tacaagcaaa acaggctttt actttctcgc
     4801 caacttacaa ggcctttctc agtaaacagt atatgaccct ttaccccgtt gctcggcaac
     4861 ggcctggtct gtgccaagtg tttgctgacg caacccccac tggttggggc ttggccatag
     4921 gccatcagcg catgcgtgga acctttgtgt ctcctctgcc gatccatact gcggaactcc
     4981 tagccgcttg ttttgctcgc agcaggtctg gagcaaacct catcgggacc gacaattctg
     5041 tcgtactctc ccgcaagtat acatcgtttc catggctgct aggctgtgct gccaactgga
     5101 tcctgcgcgg gacgtccttt gtttacgtcc cgtcggcgct gaatcccgcg gacgacccct
     5161 cccggggccg cttggggctc taccgcccgc ttctccgtct gccgtaccgt ccgaccacgg
     5221 ggcgcacctc tctttacgcg gactccccgt ctgtgccttc tcatctgccg gaccgtgtgc
     5281 acttcgcttc acctctgcac gtcgcatgga ggccaccgtg aacgcccacc ggaacctgcc
     5341 caaggtcttg cataagagga ctcttggact ttcagcaatg tcatctcagt ggctaataaa
     5401 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     5461 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     5521 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     5581 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     5641 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     5701 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     5761 ttctcttatg gagatctttg catttttaga catttagaag cctatatctt gttacagaat
     5821 tggaattaca caaaaattct accatatttt gaaagcttag gttgttctga aaaaaacaat
     5881 atattgtttt cctgggtaaa ctaaaagtcc cctcgaggaa aggcccctaa agtgaaacag
     5941 tgcaaaacgt tcaaaaactg tctggcaata caagttccac tttgaccaaa acggctggca
     6001 gtaaaagggt taagaagact gtcagccttg agcggtatca gctcactcaa aggcggtaat
     6061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6541 gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag
     6601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6781 cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagttagaaa
     6841 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     6901 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     6961 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     7021 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     7081 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     7141 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     7201 gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgagtgcaac
     7261 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     7321 aatacctgga acgctgtttt tccggggatc gcagtggtga gtaaccatgc atcatcagga
     7381 gtacggataa aatgcttgat ggtcggaagt ggcataaatt ccgtcagcca gtttagtctg
     7441 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     7501 ggcgcatcgg gcttcccata caagcgatag attgtcgcac ctgattgccc gacattatcg
     7561 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgac
     7621 gtttcccgtt ggatatggct cattttttac ttcctcacct tgtcgtatta tactatgccg
     7681 atatactatg ccgatgatta attgtcgaca ctgcgggggc tctgtgtggt aagcaggtct
     7741 taaccttttt actgccaatg acgcatggga tacgtcgtgg cagtaaaagg gcttaaatgc
     7801 caacgacgcg tcccatacgt tgttggcatt ttaattcttc tctctgcagc ggcagcatgt
     7861 gccgccgctg cagagagttt ctagcgatga cagcccctct gggcaacgag ccgggggggc
     7921 tgtctttctt tatgttttaa atgcactgac ctcccacatt ccctttttag taaaatattc
     7981 agaaataatt taaatacatc attgcaatga aaataaatgt tttttattag gcagaatcca
     8041 gatgctcaag gcccttcata atatccccca gtttagtagt tggacttagg gaacaaagga
     8101 acctttaata gaaattggac agcaagaaag cgagtcaggc accgggcttg cgggtcatgc
     8161 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
     8221 cgtagaaggg gaggttgcgg ggcgcggatg tctccaggaa ggcgggcacc ccggcgcgct
     8281 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
     8341 gcgacacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
     8401 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
     8461 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg
     8521 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
     8581 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
     8641 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
     8701 tggcgaggcg caccgtgggc ttgtactcgg tcatggtggc ggacgaaagg cccggagatg
     8761 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc
     8821 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
     8881 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
     8941 cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga
     9001 gtcgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact
     9061 tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt
     9121 ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag
     9181 tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta
     9241 cccggtagag aaacttgatc tgtcgccgca attcaaactt cgtgaggctc cggtgcccgt
     9301 cagtgacctg ctatactctg gaga
//
