LOCUS       sequence-357083-        4718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 
            central TetO in addition to carrier DNA that prevents overassembly 
            of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_193 NRL + TetO
FEATURES             Location/Qualifiers
     source          1..4718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    802..820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2371..2462
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3398..3985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3886..3905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4026..4047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4062..4067,4068..4085,4086..4092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4100..4116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4105..4127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4124..4140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4124..4140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4275..4297
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4289..4306
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4290..4306
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4313..4332
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4313..4331
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccagatcttc gaacatttga attccggccg tttcaggtag cttgctaaga acaggatgta
       61 tatatctgac acgtgcctgg agactaggga gtaatcccct tggcggttaa aacgcggggg
      121 acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg
      181 gcaccgggat tctccagaaa tcaactttga ggaattcgac gtcaaagtct ttactatatg
      241 caaacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc ccttggcggt
      301 taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa
      361 ttgagcggcc tcggcaccgg gattctccag ttctcgaaac accggaattc ggtaccatat
      421 ccaaaaccgc tccttcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
      481 tccccttggc ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc
      541 tgtctacgac caattgagcg gcctcggcac cgggattctc cagagcattg aaatctcgaa
      601 ttccccgggc tcttttggac aaccacccaa caggatgtat atatctgaca cgtgcctgga
      661 gactagggag taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa
      721 gcggtgctag agctgtctac gaccaattga gcggcctcgg caccgggatt ctccagacat
      781 gtgaattcga gctctgtgtg atctctatca ctgataggga acacaacagg atgtatatat
      841 ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc gggggacagc
      901 gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
      961 gggattctcc agatgatggc cgaattcctg cagtgtgtta atttaacatc accacaggat
     1021 gtatatatct gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg
     1081 gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc
     1141 tcggcaccgg gattctccag atcaatataa taaggaattc ccatggtggg aaactccaaa
     1201 cccaatacag gatgtatata tctgacacgt gcctggagac tagggagtaa tccccttggc
     1261 ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
     1321 caattgagcg gcctcggcac cgggattctc cagtcaaact ttttgttgaa ttcaagcttt
     1381 ttatcacctt catcgcgcta caggatgtat atatctgaca cgtgcctgga gactagggag
     1441 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
     1501 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccagcata caaatgctct
     1561 gaattcatcg atttttcttc aagatattcc aaacaggatg tatatatctg acacgtgcct
     1621 ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt
     1681 taagcggtgc tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccaga
     1741 agaagtcgag tgggaattcg gatcccaagg cccttaattt tccaaacagg atgtatatat
     1801 ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc gggggacagc
     1861 gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg cctcggcacc
     1921 gggattctcc agtaacctag tataggggac atgatatctg cagaattcag gcctcaagag
     1981 gaattatacg aagcctgtga aaaggttgtt ttggaactta gaaactacac tgagcattct
     2041 acaccatttc tgaataaagt gagcaaaaga gaagccccca attatcatca aatcatcaaa
     2101 aagtccatgg acctgaatac tgttttaaaa aaactgaaaa gctttcaata tgactccaaa
     2161 caagaatttg tagacgatat tatgctaata tggaaaaatt gtttgaccta taattcagat
     2221 ccttcacatt ttttgagagg gcatgctatt gctatgcaga agaaatctct tcagttgatt
     2281 cgcatgattc caaataggcc tgaattccag cacactggcg gccgttacta gtagcccgcc
     2341 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata
     2401 gatatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2521 ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg
     2581 cacgagtggg atacatagaa ctggacttga atagcggtaa aatccttgag agttttcgcc
     2641 ctgaagagcg ttttccaatg atgagcactt tcaaagttct gctatgtgga gcagtattat
     2701 cccgtgtaga tgcggggcaa gagcaactcg gacgacggat atcctattcg cagaatgact
     2761 tggttgaata ctccccagtg acagaaaagc accttacgga cggaatgacg gtaagagaat
     2821 tatgtagtgc cgccataacg atgagtgata acactgcggc gaacttactt ctgacaacca
     2881 tcggtggacc gaaggaatta accgcttttt tgcacaatat gggagaccat gtaactcgcc
     2941 ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga gacaccacaa
     3001 tgcctgcggc aatggcaaca acattacgca aactgatatc tggcgaacta cttactctgg
     3061 cttcacggca acaattaata gactggcttg aagcggataa agttgcagga ccactactgc
     3121 gttcggcact tcctgctggc tggtttattg ctgataaatc tggggcagga gagcgtggtt
     3181 cacggggtat cattgccgca cttggaccag atggtaagcc ttcccgtatc gtagttatct
     3241 acacgacggg tagtcaggca actatggacg aacgaaatag acagattgct gaaatagggg
     3301 cttcactgat taagcattgg taaaccgata tcattaaagg ctccttttgg agcctttttt
     3361 tttggacgga ccgagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtatgt ttgccggatc
     3481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3541 ctgttcttct agtgtagccg gatatcggcc accacttcaa gaactctgta gcaccgccta
     3601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3781 agcgtgagct atgagaaagc gccacgttcc gatatcgaga aaggcggaca ggtatccggt
     3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctgaa
     4021 ttgattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgctgccg
     4081 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4141 tatgattacg cccaagctgt ataggctgcg caactgttgg gaagggcgat cggtgcgggc
     4201 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     4261 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     4321 ctcactatag ggcgaattgg gccctctaga gatatcctcg agcaacatga agtcaggatg
     4381 ggacaggatg tatatatctg acacgtgcct ggagactagg gagtaatccc cttggcggtt
     4441 aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat
     4501 tgagcggcct cggcaccggg attctccagt tacatgatca ccagaattct ctagatatgt
     4561 tttatgacat aaacgacagg atgtatatat ctgacacgtg cctggagact agggagtaat
     4621 ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg tgctagagct
     4681 gtctacgacc aattgagcgg cctcggcacc gggattct
//
