LOCUS       sequence-357084-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 15
            bp linker lengths in addition to carrier DNA that prevents 
            overassembly of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_15bpLinker
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        386..477
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1413..2000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1901..1920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2041..2062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2077..2082,2083..2100,2101..2107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2115..2131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2120..2142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2139..2155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2139..2155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta aaacgcgggg
       61 gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt gagcggcctc
      121 ggcaccggga ttctccagtt catgatatcg gatccccggg taccgagctc gaattcgggc
      181 ttgccgtcgt tttacagcga cgagactggg aaaatcctgg cgttacccaa cttaatcgcc
      241 ttgccgcaca cccccctttc gccagttggc gtaatagcga agaagcccgc actgaccgcc
      301 cttcccaaca gttgcgtagt ctgaatggcg aatggcgtta aactagtagc ccgcctaatg
      361 agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc aaatagatat
      421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      481 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      541 tttgctcacc cagaaacgct cgtgaaagta aaagacgcag aggaccaatt gggggcacga
      601 gtgggataca tagaactgga cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa
      661 gagcgttttc caatgatgag cactttcaaa gttctgctat gtggagcagt attatcccgt
      721 gtagatgcgg ggcaagagca actcggacga cggatatcct attcgcagaa tgacttggtt
      781 gaatactccc cagtgacaga aaagcacctt acggacggaa tgacggtaag agaattatgt
      841 agtgccgcca taacgatgag tgataacact gcggcgaact tacttctgac aaccatcggt
      901 ggaccgaagg aattaaccgc ttttttgcac aatatgggag accatgtaac tcgccttgac
      961 cgttgggaac cagaactgaa tgaagccata ccaaacgacg agcgagacac cacaatgcct
     1021 gcggcaatgg caacaacatt acgcaaactg atatctggcg aactacttac tctggcttca
     1081 cggcaacaat taatagactg gcttgaagcg gataaagttg caggaccact actgcgttcg
     1141 gcacttcctg ctggctggtt tattgctgat aaatctgggg caggagagcg tggttcacgg
     1201 ggtatcattg ccgcacttgg accagatggt aagccttccc gtatcgtagt tatctacacg
     1261 acgggtagtc aggcaactat ggacgaacga aatagacaga ttgctgaaat aggggcttca
     1321 ctgattaagc attggtaaac cgatatcatt aaaggctcct tttggagcct ttttttttgg
     1381 acggaccgag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tatgtttgcc ggatcaagag
     1501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1561 cttctagtgt agccggatat cggccaccac ttcaagaact ctgtagcacc gcctacatac
     1621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1801 gagctatgag aaagcgccac gttccgatat cgagaaaggc ggacaggtat ccggtaagcg
     1861 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1921 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1981 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctgaattgat
     2041 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc tgccggctcg
     2101 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgactatga
     2161 ttacgcccaa gcttgatatc atgaacagga tgtatatatc tgacacgtgc ctggagacta
     2221 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     2281 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttccatcta
     2341 gatggaacag gatgtatata tctgacacgt gcctggagac tagggagtaa tccccttggc
     2401 ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
     2461 caattgagcg gcctcggcac cgggattctc cagttccagc tagatggaac aggatgtata
     2521 tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa cgcgggggac
     2581 agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag cggcctcggc
     2641 accgggattc tccagttcca gctagatgga acaggatgta tatatctgac acgtgcctgg
     2701 agactaggga gtaatcccct tggcggttaa aacgcggggg acagcgcgta cgtgcgttta
     2761 agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat tctccagttc
     2821 cagctagatg gaacaggatg tatatatctg acacgtgcct ggagactagg gagtaatccc
     2881 cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
     2941 tacgaccaat tgagcggcct cggcaccggg attctccagt tccagctaga tggaacagga
     3001 tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg ttaaaacgcg
     3061 ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca attgagcggc
     3121 ctcggcaccg ggattctcca gttccagcta gatggaacag gatgtatata tctgacacgt
     3181 gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag cgcgtacgtg
     3241 cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggattctc
     3301 cagttccagc tagatggaac aggatgtata tatctgacac gtgcctggag actagggagt
     3361 aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga
     3421 gctgtctacg accaattgag cggcctcggc accgggattc tccagttcca gctagatgga
     3481 acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct tggcggttaa
     3541 aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg
     3601 agcggcctcg gcaccgggat tctccagttc cagctagatg gaacaggatg tatatatctg
     3661 acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg
     3721 tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct cggcaccggg
     3781 attctccagt tccagctaga tggaacagga tgtatatatc tgacacgtgc ctggagacta
     3841 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     3901 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca gttccagcta
     3961 gctggaacag gatgt
//
