LOCUS       sequence-357195-        7869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1710-2505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J
FEATURES             Location/Qualifiers
     source          1..7869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2530..2595
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2620..2646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5120..5161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5171..5188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5211..5228)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5261..5395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5285..5304)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5339..5358
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5996..6584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6076..6095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6755..7546,7547..7615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7378..7397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7616..7720)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7788..7806
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggtt gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggacgcaca aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa
     2581 tggaaagctg caaacggtgg atctcctcca catatggctt acccatacga tgttccagat
     2641 tacgctcctc caagcttaat taagatgtct cagccaaatg tgtttgctaa tgatgatagc
     2701 atggtatacg gggaactggc tagcaaaatg gaggccttca tgaacacggc aatcggagtg
     2761 ccgacactcc agatccagac tagcaagatg cacatgctac tcaacgccct gatagcgacg
     2821 cgtcgactgc gtgaccaaga gtctgccttt aatctgctga cccgcgcggt cgagggtttg
     2881 accgaaggat tggtcaatat gcatgaaaac atggagcaaa tgaagatgta ccaaaacatc
     2941 catctccgta tccttggctt gctgaacaac agtttcggtg ctccaaacac agagcgagcg
     3001 gtaacaaagt gtttcttcga tatccgagag gaggtgcgat ataacgtgga ggcagctcgc
     3061 gctttgatta cgtcgcactt tgtcaatctg aatcagtttg acggaatgct acgcgactgc
     3121 atggacaacg gaaacaatta tgtggccata tcgttcggta tcgcactgct ggagcgcctc
     3181 atcatggacg atcgagtgat taatattgtt tcggacaacg aattcatggc aactgttgag
     3241 ctgctaggta ggctcactca gcatcgccat cggtatccag aatgcattgt gaatgctatt
     3301 gatacgctct ggagcggaaa ctttaactcg agcagcgact acagcccgtt caacggcaat
     3361 gatcgttact tgtcgggagc ctcccattac atccactccg gcatgcatca cgtaaggtca
     3421 tgcgatacag atgatccgcc gggcttacaa gagaagacgg agtttctgct taaggattgg
     3481 gttgcactgt acacgcaaca gaaccagcag tcaacgcgcg atgcacgcaa cttcagtgca
     3541 tttgttcaga agatgaacac atatggtatc ctgaaaacgg acgacttgat cacccgtttt
     3601 ttccgccagg ccacacacat ttgcacggat gtggtgtaca gaatgttcgc cgagccgagt
     3661 ttaccaatca accaggccaa aaacaagata ttccagtgga tcgatgcgtt cgtccatctg
     3721 attgcaatgc tcgtgcgaca ctcaggcgaa gcaggtaatc caaccaccaa gatcaacctg
     3781 ttaaacaagg tgctgggcat tgtgctggga acgctgatta aggaccatga gatgcgtggc
     3841 gttagcttcc agcaggttgg ctaccatcgc ttcttcatga tgctgttcat ggagctgtgc
     3901 acggccgatg tgattctcga gtccctgatg cacagtattg tgtcggcctt cgcctacaca
     3961 tatcacttgt tgaatcccag cgtggctccg ggattctgct ttgcttggtt ggagctcatt
     4021 tcgcatcgcg tcttcctggg ccgcatcctg gtccaaattc ccgggcaaaa gggctggcca
     4081 ctttacgctc aattgctgca ggaccttttt aaatatcttg cgcccttctt acgcaacact
     4141 gagctcggca aaccagtcca gcttttgtac aaggggacgc tacgtgttct gctcgttctg
     4201 cttcacgact tcccggagtt cttgtgcgac tatcattttg ggttctgtga caccatcccg
     4261 cccaattgcg tccagatgcg taacatcatc ctgtcggcgt ttccgcgcaa catgcgcttg
     4321 cccgatccct tcacgcccaa cctaaaagtg gacatgctgt cggacagtag taatgcgcct
     4381 aaggtgctca gtagctacat tatgaacatc cagcccgcga actttaagaa ggatttggac
     4441 tcgtacctca aggcccgggc gccagttact tttctatcgg aattacgtgg tcacttgcag
     4501 gtgacaagcg agccaggaac tcgttacaac atggcgctga tgaatgctct ggtcatgtat
     4561 gtgggaactc aggcgattgc tttgatcaga aacaaaaact ttgtgcccaa cacctcgaac
     4621 atagcacaca gcgcccacat ggatatcttc cagaatcttg ctgttgactt ggatacggag
     4681 ggtcgctatc tgttcttgaa tgcgattgcg aaccagttgc gctatcccaa cagccacacg
     4741 cactacttca gctgtgcggt gctgcatctg tttgccgagg ccaactcgga ggcaattcag
     4801 gagcagatca ctcgcgtact tctggaacga ttgatcgtga accgcccgca tccttgggga
     4861 ctgttgatca cttttattga gctgatcaaa aatcccatct acaagttctg ggaccatgac
     4921 tttgtgcact gtgcgccaga gatcaccaag ctctttgaat cggtggcccg ttcctgtctg
     4981 gccaagtcga acgtcaccca gcagttgaac atgcccgtcg tcgatggcga gggccaggag
     5041 gtagccacca tcaactgatc tagacaaagg gcgaattcca gcacactggc ggccgttact
     5101 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5161 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     5221 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     5281 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5341 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5401 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     5461 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga
     5521 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     5581 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     5641 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     5701 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     5761 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     5821 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     5881 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     5941 agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6961 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7021 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     7081 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7141 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7201 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7261 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7321 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     7381 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7441 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7501 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7561 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7681 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7741 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7801 cgaggccctt tcgtcttcaa gaattaattc tatattctaa aaacacaaat gatacttcta
     7861 aaaaaaaat
//
