LOCUS       sequence-357200-       13551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, 
            Izaurralde E
  TITLE     The interactions of GW182 proteins with PABP and deadenylases are 
            required for both translational repression and degradation of miRNA 
            targets.
  JOURNAL   Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. 
            Epub 2012 Nov 21.
  PUBMED    23172285
REFERENCE   2  (bases 1 to 13551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks1078"; journalName: "Nucleic Acids Res"; date: 
            "2013-01"; volume: "41"; issue: "2"; pages: "978-94"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot1_J
FEATURES             Location/Qualifiers
     source          1..13551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10802..10843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10853..10870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10893..10910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10943..11077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10967..10986)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11021..11040
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11678..12266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11758..11777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12437..13228,13229..13297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13060..13079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13298..13402)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13470..13488
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttaattaa gatgaacgta gagagccaac tgaagacacc gccaacgcac
     3301 attcgaaatc tggtcaagca cgtgaacaag cgaaatttta atgagagcag cgagcaaata
     3361 aatcagttcg ttaaagagca cggcctggaa gcggatcgca gcagcctgcg ccacctgttc
     3421 tccgtgatca acttcagcga tctcgtgcca tcagttacgg tccagctgca ggcgaagctc
     3481 ctgggcatcc agttggagcg tcagcttcac agttcatcgt ttgtgtccaa catctgctac
     3541 gcttttgacc agttcttcgc cagcaaccag aagtccctga agcctgttgc ggtagcggat
     3601 ctcgttggcc aggtcgcccg actgaccggc atcaacaagg tttgtgagtg cgtctttgcc
     3661 ttggccttga cccactcctc gtacacagaa ctcaaacatt ctgccaggaa caacctaaaa
     3721 ggtagcctca gcgaacttat agactcttac ttgggtaata aaggcaccgg cccagccgac
     3781 agcggtctcc gggagatctc cttcgatcta ctgcagtatt tgctgtgctg ccttagcgag
     3841 tacgtacaac cgcaggtgga agcacagttt ttgatcaagc ttcgtgagga gtttccgcgc
     3901 caggcggtac cgctggttct agcgccgttc ctttacggct cgacgacggc gacgattgcg
     3961 ggagcaggtg ccagcgaaac ggatgcggag gccgaagcga cgactaatag caacagctcc
     4021 agtttcgaag cagatgcttt gaacgaggtg ggaatcgagg atatttatga ccatttaagc
     4081 gagataatat tcaccaacca gggcaaaaat aatattatgg acacatcttg gattaatcta
     4141 atccttgaaa tcggttatga atttacatcg agcgttgaag agtgtaagaa tcatttgtgt
     4201 tcccgcgagc gagaacgcgc tgagctccag tccaaagatg tcgccaagat cgtgggtctt
     4261 atgtgccgac ggcactcctc cctgctcgat tgtaatgtga atctaccgac gccggcgaac
     4321 ttctggcctg gtcagggaca gggcggtggc agcaacagca gtggctcttc gcagacacaa
     4381 atcactccgc agcaacagaa cccgggcagc agtaacaaca acgatggcag cgatggcaat
     4441 tcgtccagtg ataagaagga caagaaggag acgacggagg ccacgcaaac ttggaagcca
     4501 gatgtctttg tgcaggcgct caaggaggtg gtgcctcagc tcaactggaa ggacgtgtgc
     4561 atggaacttg atcatcccga gttcgtgctg aaggatcgca tcggtttgga gcttttgctc
     4621 accatccttc ggctggccac tggctctaac atatttccac atccagaatg catttaccgt
     4681 cactgggcaa acacggaagg tcagctgtcg cttatcgcga caatgctgaa gaacccggat
     4741 ctcttttcct tcgccgactt tgtatttagc cagccagcgt tggatgtgct taagacagct
     4801 ccggatgcgg acaataagga gatctcgtcc tggaagtcgc ttcacctggt ggaggtgctg
     4861 ctctccattg cggacaaggg atactatacg caggtccatg agctattcaa gttcccagcg
     4921 cagaactgtc ccgatgtgct atttttggcc ttattgaaca caagtccgcc tatgacgcca
     4981 ctgcgtcagg atctgtttaa ccaactgata ccaacgttcc ttggcaacca tcccaactcg
     5041 aacgtgatct tggccagcgc ctggagctcg aacagcttcc aacttcgttc caatatcatg
     5101 aatgctatgt ccgagtggta tctccgaggc aatgagttcg atcaggtgaa actgtcgcgc
     5161 atcctggatc ttgcccagga tttaaaagcg ctatcagcgc tgctcaacgc ccgttcattt
     5221 ttattcatta tcgacctggc ttgccttgcc tctcggcgcg agtacctgaa gctggaaaag
     5281 tggcttaccg ataaaattcg cgaacatgga gagcctttta tgcaggctat aatcaaggtt
     5341 ctgcaccgcc gctgcccgca ggttatcaac gccaaagtgc cagaggatca gctgcctccc
     5401 aagcaggcgc agcttttgcc cgagacagtt acaaccatga tcaactgtct gcaaacgtgc
     5461 atcaataact gcatgcagcc ggagatggtc gaagtcatca tgcaaatgac cgcgaacgtg
     5521 gcgattatgg ctaacatggc gcgtgcccaa cagcagcagc agccaggatt ggttccgccg
     5581 cctccaccaa caattctgcg cggccatcgt ggtatggatc tgcctggagg catagtccct
     5641 ccgccaccgc agcaaccgtt ctccggaaac ctcaacgcgc agatgtttgg accaggaatg
     5701 gatccgctaa cgaatatgtc caacaactta gccggcctca acctcagcgg cccgaacgga
     5761 gcatttaatt ttggaaacat gctaacttct ccatcgcgtc tgatgactcc gggagccaat
     5821 ccctatccgc tgaacctcat gcagatgccg caggctccgc caccacccaa cgtaggaaac
     5881 ctaggccgca tgctgccagg gggtccacaa cagcagactc caacgccgac tccaacggcc
     5941 cccaatccaa acaaccctgt gatggccgat ctccagatac ctgtgtcaaa ggaagttgaa
     6001 gacgaggtca actcatactt tcagcgcatc tacaaccacc agccgaatcc aacgctgtcc
     6061 atcgatgaag tgctggacat tttgcaaaga tttaaagagt caagtaaccg gcgcgagcag
     6121 gaagtctttt tgtgcatgct gcgcaatctg ttcgaggaat atcgcttttt ctgtcaatac
     6181 ccagagaaag agctacagat tactgcgcag ctatttggtg gcattattga tcgtaacctg
     6241 gtgcccactt ttgttgcact gggcctttct ctgcgctgtg tcttggatgc cctccgcaag
     6301 cccgatggat ctaagcttta ctatttcggt gtgacggcac tggacagatt cagaactcga
     6361 ttgcacacct acaacaaata ctgcgagcat atccgctcca ttcctcactt ctctgacttc
     6421 ccgccgcacc ttattcagta tgtggaatac ggaatgcacg gccaggagcc gccgccgcag
     6481 aagttgattg gactcagcaa tacgattcct tcggcgatat ctagcggacc aggaacggaa
     6541 ccaatttaca ggaatagctc catgttaggt aatatgcccg ctgccacccc gggatcggga
     6601 ccgaaatcaa acgctgctgt atcgcatgcc acgagaatga agtccatcgc caatgccacc
     6661 aatatcgaca cacttttggt ggccaaccag gaagagaaag tgactgtgcc accagagcct
     6721 gtgcaggaca aaactgcctt tattttcaac aacttgagcc agctgaacat accgcagaag
     6781 tgcgatgaga taaaggagat tatgaccaag gagtattggc cgtggctagc gcaatatctg
     6841 gtcctcaaac gcgcatcaat ggagttcaac ttccacacac tatattacaa ctttttggat
     6901 gcccttaaaa acggcgagat caaccgattc gtaaccaaag agaccctacg caacatcaag
     6961 gtactcttgc gctccgataa gggagttatt aatttctccg atcgaagtct gttgaagaac
     7021 ctcggacact ggttgggcat gatgacctta ggtcgcaatc gccccatcct gcagttggat
     7081 ctggatctta aatccctttt ggctgaggct taccacaagg gccagcagga actgcttttt
     7141 gtggttcctt tcgtagccaa gatccttgag tcatccgcca agtctcgcat cttccgatcg
     7201 cccaatccat ggacaatggg tatcatgtac gtgcttgctg agcttcacca ggagccagac
     7261 cttaagctga acctgaagtt tgaaatcgaa gtactctgca aaacacttaa tctggagttg
     7321 gccaagttgc gacaagtgat ctacctgaag gatcccaatc gaactcaccg gattgaagag
     7381 cagatgtcgc aaccaaaacc gaaacaactt gagccagtgg cttctgcgcc agctctaccg
     7441 cgtgaacagc aatccccggc acaacctccg ccaccaccac aacagcagca accaccgcag
     7501 cagcaggtac ctccaccgcc atcctctgcg gacgtggacg cccaaaatgc ggccgctatg
     7561 atgatggcag cgggcggagc taatagtact cctggatcgg tatcttcgcc caatctaccc
     7621 accgattcca gccaggtggc actaccgcca ccagagccgc gctattcata cgtggacgta
     7681 aacgtgagca acttccagct tattggtcag cagttggtac tgccacccaa tactccgttc
     7741 ctgcacgcca atcccggaat caaacacatg gtcgtcaacg ccgtggagcg cacgataacc
     7801 gactggttgc agcctattgt ggaccgaagc atccgcattg cctgtgccac caccgagcag
     7861 atcatccgca aggacttcgc cctggatgct gacgagaacc gaatgcgcac tgccgctcat
     7921 cagatggtac gaaaccttgc cgccggcatg gccatgatta ccggtaagga tgagatagcg
     7981 cgtgctatta gccagaatct gcacaaggcc ttgctgtcgg gtctaaatgg gatgcccagc
     8041 atggctgaga tccaagctgc tgctatgcag ttagccagcg aaaatgttga gctagtttgc
     8101 gctttcattc agaaaacatc tgctgaaaag gcagccgcag agatcgatag gcgtctaagc
     8161 accgatttcg agaccagaaa aattgctcgt gaagagggca accgctttgt ggacgcccag
     8221 atcctcacct atcaacagga gcgtcttcca gaagcagtgc gcatcaaggt gggcgcggct
     8281 ccagctacac tttatgctgt gtactcggag ttcgctagaa gcatacctgg cttccagcag
     8341 atgagcgatc gcgatattgc cctgtttgtg cccaagccga cggatttgtc tcagccaaat
     8401 gtgtttgcta atgatgatag catggtatac ggggaactgg ctagcaaaat ggaggccttc
     8461 atgaacacgg caatcggagt gccgacactc cagatccagg ctagcaagat gcacatgcta
     8521 ctcaacgccc tgatagcgac gcgtcgactg cgtgaccaag agtctgcctt taatctgctg
     8581 acccgcgcgg tcgagggttt gaccgaagga ttggtcaata tgcatgaaaa catggagcaa
     8641 atgaagatgt accaaaacat ccatctccgt atccttggct tgctgaacaa cagtttcggt
     8701 gctccaaaca cagagcgagc ggtaacaaag tgtttcttcg atatccgaga ggaggtgcga
     8761 tataacgtgg aggcagctcg cgctttgatt acgtcgcact ttgtcaatct gaatcagttt
     8821 gacggaatgc tacgcgactg catggacaac ggaaacaatt atgtggctat atcgttcggt
     8881 atcgcactgc tggagcgcct catcatggac gatcgagtga ttaatattgt ttcggacaac
     8941 gaattcatgg caactgttga gctgctaggt aggctcactc agcatcgcca tcggtatcca
     9001 gaatgcattg tgaatgctat tgatacgctc tggagcggaa actttaactc gagcagcgac
     9061 tacagcccgt tcaacggcaa tgatcgttac ttgtcgggag cctcccatta catccactcc
     9121 ggcatgcatc acgtaaggtc atgcgataca gatgatccgc cgggcttaca agagaagacg
     9181 gagtttctgc ttaaggattg ggttgcactg tacacgcaac agaaccagca gtcaacgcgc
     9241 gatgcacgca acttcagtgc atttgttcag aagatgaaca catatggtat cctgaaaacg
     9301 gacgacttga tcacccgttt tttccgccag gccacacaca tttgcacgga tgtggtgtac
     9361 agaatgttcg ccgagccgag tttaccaatc aaccaggcca aaaacaagat attccagtgg
     9421 atcgatgcgt tcgtccatct gattgcaatg ctcgtgcgac actcaggcga ggcaggtaat
     9481 ccaaccacca agatcaacct gttaaacaag gtgctgggca ttgtgctggg aacgctgatt
     9541 aaggaccatg agatgcgtgg cgttagcttc cagcaggttg gctaccatcg cttcttcatg
     9601 atgctgttca tggagctgtg cacggccgat gtgattctcg agtccctgat gcacagtatt
     9661 gtgtcggcct tcgcctacac atatcacttg ttgaatccca gcgtggctcc gggattctgc
     9721 tttgcttggt tggagctcat ttcgcatcgc gtcttcctgg gccgcatcct ggtccaaatt
     9781 cccgggcaaa agggctggcc actttacgct caattgctgc aggacctttt taaatatctt
     9841 gcgcccttct tacgcaacac tgagctcggc aaaccagtcc agcttttgta caaggggacg
     9901 ctacgtgttc tgctcgttct gcttcacgac ttcccggagt tcttgtgcga ctatcatttt
     9961 gggttctgtg acaccatccc gcccaattgc gtccagatgc gtaacatcat cctgtcggcg
    10021 tttccgcgca acatgcgctt gcccgatccc ttcacgccca acctaaaagt ggacatgctg
    10081 tcggacagta gtaatgcgcc taaggtgctc agtagctaca ttatgaacat ccagcccgcg
    10141 aactttaaga aggatttgga ctcgtacctc aaggcccggg cgccagttac ttttctatcg
    10201 gaattacgtg gtcacttgca ggtgacaagc gagccaggaa ctcgttacaa catggcgctg
    10261 atgaatgctc tggtcatgta tgtgggaact caggcgattg ctttgatcag aaacaaaaac
    10321 tttgtgccca acacctcgaa catagcacac agcgcccaca tggatatctt ccagaatctt
    10381 gctgttgact tggatacgga gggtcgctat ctgttcttga atgcgattgc gaaccagttg
    10441 cgctatccca acagccacac gcactacttc agctgtgcgg tgctgcatct gtttgccgag
    10501 gccaactcgg aggcaattca ggagcagatc actcgcgtac ttctggaacg attgatcgtg
    10561 aaccgcccgc atccttgggg actgttgatc acttttattg agctgatcaa aaatcccatc
    10621 tacaagttct gggaccatga ctttgtgcac tgtgcgccag agatcaccaa gctctttgaa
    10681 tcggtggccc gttcctgtct ggccaagtcg aacgtcaccc agcagttgaa catgcccgtc
    10741 gtcgatggcg agggccagga ggtagccacc atcaactgat ctagagggcc cgcggttcga
    10801 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
    10861 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc
    10921 tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca
    10981 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
    11041 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
    11101 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
    11161 atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca
    11221 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
    11281 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
    11341 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
    11401 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
    11461 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
    11521 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
    11581 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa
    11641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    11701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    11761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    12001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    12061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    12121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    12181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    12241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    12301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    12361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    12541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    12601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    12661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    12721 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
    12781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    12841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    12901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    12961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    13021 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    13081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    13141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    13201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    13261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    13321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    13381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    13441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    13501 aagaattaat tctatattct aaaaacacaa atgatacttc taaaaaaaaa t
//
