LOCUS       sequence-357207-        8947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a 
            pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Berlew EE, Yamada K, Kuznetsov IA, Rand EA, Ochs CC, Jaber Z, 
            Gardner KH, Chow BY
  TITLE     Designing Single-Component Optogenetic Membrane Recruitment Systems:
            The Rho-Family GTPase Signaling Toolbox.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):515-521. doi: 
            10.1021/acssynbio.1c00604. Epub 2022 Jan 3.
  PUBMED    34978789
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00604"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "515-521"
COMMENT     Sequence Label: opto-Itsn1
FEATURES             Location/Qualifiers
     source          1..8947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             140..934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(194..213)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     804..823
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1108..1229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1145..1164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1199..1218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1278..1294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1278..1294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1291..1313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1302..1318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1326..1332,1333..1350,1351..1356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1371..1392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1509..1526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1680..2268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1760..1779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2439..3230,3231..3299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFHVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3062..3081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3300..3404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3479..3498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3670..4049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4050..4253
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4204..4224
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4298..4317
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4298..4316
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7228..7938
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7373..7391)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7641..7660
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7973..7990)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7979..8203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8249..8677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8336..8355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8546..8567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8686..8706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8691..8947,1..73)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8871..8947,1..59)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     join(8933..8947,1..5)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
       61 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
      121 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
      181 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
      241 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
      301 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
      361 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
      421 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
      481 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
      541 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
      601 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
      661 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
      721 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
      781 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
      841 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
      901 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
      961 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     1021 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     1081 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     1141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1201 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     1261 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1321 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1381 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1441 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1561 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1621 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1681 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1741 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1801 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1861 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1921 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1981 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2041 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2101 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2161 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2221 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2281 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2341 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2401 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2461 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2521 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2581 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2641 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2701 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2761 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2821 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2881 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2941 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3001 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3061 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3121 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3181 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3241 ggaaggcaaa atgccgcaaa aaagggaata agggcgacat ggaaatgttg aatactcata
     3301 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3361 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3421 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     3481 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     3541 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     3601 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     3661 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     3721 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     3781 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     3841 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     3901 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     3961 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     4021 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4081 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4141 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4201 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     4261 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     4321 cccaagctgg ctagcgatat gttgacccca actgaaagaa agcgacaagg atacatccac
     4381 gagctcattg tcaccgagga gaactatgtg aatgacctgc agctggtcac agagattttt
     4441 caaaaacccc tgatggagtc tgagctgctg acagaaaaag aggttgctat gatttttgtg
     4501 aactggaagg agctgattat gtgtaatatc aaactactaa aagcgctgag agtccgcaag
     4561 aagatgtccg gggagaagat gcctgtgaag atgattggag acatcctgag cgcacagctg
     4621 ccgcacatgc agccctacat ccgcttctgc agccgccagc tcaacggggc tgccctgatc
     4681 cagcagaaga cggacgaggc cccagacttc aaggagttcg tcaaaagatt ggcaatggat
     4741 cctcggtgta aagggatgcc actctctagt tttatactga agcctatgca acgggtaaca
     4801 agatacccac tgatcattaa aaatatcctg gaaaacaccc ctgaaaacca cccggaccac
     4861 agccacttga agcacgccct ggagaaggcg gaagagctct gttcccaggt gaacgaaggg
     4921 gtgcgggaga aggagaactc tgaccggctg gagtggatcc aggcccacgt gcagtgtgaa
     4981 ggcctgtctg agcaacttgt gttcaattca gtgaccaatt gcttggggcc gcgcaaattt
     5041 ctgcacagtg ggaagctcta caaggccaag agcaacaagg agctgtatgg cttccttttc
     5101 aacgacttcc tcctgctgac tcagatcacg aagcctttgg ggtcttctgg caccgacaaa
     5161 gtcttcagcc ccaaatcaaa cctgcagtat aaaatgtata aaacacctat tttcctaaat
     5221 gaggttctag taaaattacc caccgaccct tctggagacg agcccatctt ccacatctcc
     5281 cacattgacc gcgtctatac tctccgagca gaaagcataa atgaaaggac tgcctgggtg
     5341 cagaaaatca aagctgcttc tgaactctac atagagactg agaaaaagaa gggtggtgga
     5401 tcaggtggag gatccatggc cacagacgca atcgaccatt actaccagaa cagagacggc
     5461 cccccttcca ggagctttag cgacgaatac gactcataca ataagacagc ccctctgcca
     5521 cctctgagcg cctgcccaga gcgcgcccag acccctaaca gcaagagaat ccccatcaca
     5581 cccaattccg ccaagagcac ctccgagtct cctcggagaa tcacattctc tcgcgacgca
     5641 ggcagctcca ccaagggcag cacccagaca agcccatcca accagggctc cgtgtctagc
     5701 cggaatggct ctaacggaaa tggcctgggc ctgtcctctg ccggcaactt cgccgatttc
     5761 tttagctccg aggtgtttta tatcgtgctg cacaatccca ccacagccca cagattcctg
     5821 aagttttgcc agagcagggc ctgttccgag aacctggagt ttctgcagaa gatcgacgag
     5881 tacaataggc tgatcgatga ggccacacgc ctgctgtcta ccatctacag cacatacgtg
     5941 agcagcgagg cccctaagca gatcaacatc tctaatagcc acgccaggcg cctgagccac
     6001 gacatcaagg gagcaaccca caacgtgctg cccggcctgg aggatatctt caatggctcc
     6061 caggagcaga tcgagaagct gctggcctct gacctgtatc ctagatttgt gaagcaccag
     6121 gtgaccgcat ccgccacaat ggccctggca aacgacaagg agaggtaccc aggcctgggc
     6181 gattgcttct gtctgacaga cccacggaga gccgataatc ccatcgtgtt cgcaagcgac
     6241 ggattcgtga gcgtgaccgg ctattcccgc tctgatatca tcccaaggaa ctgccgcttc
     6301 ctgcagggca gctttaccga ccggcaggcc acaaagaggc tgcgcacctc catcgagaat
     6361 cgcgaggaga cagtggagct gctgctgaac tatcggaaga atggcgatcc cttttggaac
     6421 ctgctgtacg tgtcccctct gctggacggc aatggcgatg tgagattctt tctgggcggc
     6481 cagatcaact gttctaccac aatccacagc cggaccgacg tgctgagaat cctgagcctg
     6541 aacgacgagg atacaggctc cttcatcgag ggctctaata agacccccag cgtgcggtcc
     6601 aaggagtcta acaatgcctc caactatggc aagacatcct tctttaagtc ttttaagaag
     6661 tacaaggcca ataccgtgga gatcagagac gaggccggca tggagggcga gctgatcgat
     6721 aggatcggca agctgcagtt caagacacag gtggaggagt tttacaccgc ctattctaag
     6781 tacctggtgc tgagctatca gccacagtcc cagaacctga tcgtgaagta cttcagcccc
     6841 ggcatcgtgg atatgctgaa cctgaatatg cctaatggcc tggtggcccc aatcgtggac
     6901 aaggatatct tcaaggtgct gacagagcac tccccttcct ctgtgccacg gacctttaag
     6961 aacgtggtga gggaaggcat caaagcaggc agggccgtgt ctgtggagac aggcctgctg
     7021 acaggcatcg aggagatcaa gaagtctcag aacatcttca gcggcaccac agccaatcgg
     7081 gagagaggct ggaagaaggc cgaggagaag tacgtgtgcc actttacccc ctgtaaggat
     7141 gagatgggca gggtcggctg ggtggtgctg actgtcgctc ctaaactgga aagatttgaa
     7201 ttcggtggtg gatcaggtgg aggatctatg gtgagcaagg gcgaggagga taacatggcc
     7261 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7321 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7381 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7441 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     7501 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7561 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7621 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     7681 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     7741 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     7801 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     7861 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     7921 gacgagctgt acaagtaatg atctagaggg cccgtttaaa cccgctgatc agcctcgact
     7981 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8041 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8101 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8161 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8221 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8281 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8341 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8401 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8461 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8521 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8581 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8641 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     8701 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8761 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8821 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8881 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8941 cagaggc
//
